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    products/primary-antibodies/btk-antibody-y440-ab32555.pdf

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Signal Transduction Protein Phosphorylation Tyrosine Kinases Other
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RecombinantRabMAb

Recombinant Anti-BTK antibody [Y440] (ab32555)

  • Datasheet
  • SDS
Reviews (1)Q&A (1)References (1)

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Western blot - Anti-BTK antibody [Y440] (ab32555)
  • Immunoprecipitation - Anti-BTK antibody [Y440] (ab32555)
  • Anti-BTK antibody [Y440] (ab32555)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [Y440] to BTK
  • Suitable for: WB, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
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Recombinant human BTK protein (ab205800)
Primary
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Anti-SMG6 antibody (ab87539)

View more associated products

Overview

  • Product name

    Anti-BTK antibody [Y440]
    See all BTK primary antibodies
  • Description

    Rabbit monoclonal [Y440] to BTK
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human BTK aa 600-700 (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: Daudi cell lysate, K-562 cell lysate, U-937 cell lysate.
  • General notes

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    Y440
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Other
    • Immunology
    • Innate Immunity
    • TLR Signaling

Associated products

  • Alternative Versions

    • Anti-BTK antibody [Y440] - BSA and Azide free (ab247268)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Daudi whole cell lysate (ab3951)
  • Recombinant Protein

    • Recombinant human BTK protein (ab205800)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab32555 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000. Predicted molecular weight: 76 kDa.
IP
1/30.
Notes
WB
1/1000. Predicted molecular weight: 76 kDa.
IP
1/30.
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IHC.

Target

  • Function

    Plays a crucial role in B-cell ontogeny. Transiently phosphorylates GTF2I on tyrosine residues in response to B-cell receptor cross-linking. Required for the formation of functional ARID3A DNA-binding complexes.
  • Involvement in disease

    Defects in BTK are the cause of X-linked agammaglobulinemia (XLA) [MIM:300755]; also known as X-linked agammaglobulinemia type 1 (AGMX1) or immunodeficiency type 1 (IMD1). XLA is a humoral immunodeficiency disease which results in developmental defects in the maturation pathway of B-cells. Affected boys have normal levels of pre-B-cells in their bone marrow but virtually no circulating mature B-lymphocytes. This results in a lack of immunoglobulins of all classes and leads to recurrent bacterial infections like otitis, conjunctivitis, dermatitis, sinusitis in the first few years of life, or even some patients present overwhelming sepsis or meningitis, resulting in death in a few hours. Treatment in most cases is by infusion of intravenous immunoglobulin.
    Defects in BTK may be the cause of X-linked hypogammaglobulinemia and isolated growth hormone deficiency (XLA-IGHD) [MIM:307200]; also known as agammaglobulinemia and isolated growth hormone deficiency or Fleisher syndrome or isolated growth hormone deficiency type 3 (IGHD3). In rare cases XLA is inherited together with isolated growth hormone deficiency (IGHD).
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
    Contains 1 Btk-type zinc finger.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • Post-translational
    modifications

    Autophosphorylated on Tyr-223 and Tyr-551. Phosphorylation of Tyr-223 may create a docking site for a SH2 containing protein.
  • Cellular localization

    Cytoplasm. Membrane. Nucleus.
  • Target information above from: UniProt accession Q06187 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 695 Human
    • Omim: 300300 Human
    • SwissProt: Q06187 Human
    • Unigene: 159494 Human
    • Alternative names

      • Agammaglobulinaemia tyrosine kinase antibody
      • AGMX 1 antibody
      • AGMX1 antibody
      • AT antibody
      • ATK antibody
      • B cell progenitor kinase antibody
      • B-cell progenitor kinase antibody
      • BPK antibody
      • Bruton agammaglobulinemia tyrosine kinase antibody
      • Bruton tyrosine kinase antibody
      • Bruton’s Tyrosine Kinase antibody
      • Btk antibody
      • BTK_HUMAN antibody
      • dominant-negative kinase-deficient Brutons tyrosine kinase antibody
      • IMD 1 antibody
      • IMD1 antibody
      • MGC126261 antibody
      • MGC126262 antibody
      • OTTHUMP00000063593 antibody
      • PSCTK 1 antibody
      • PSCTK1 antibody
      • truncated Bruton agammaglobulinemia tyrosine kinase antibody
      • Tyrosine protein kinase BTK antibody
      • Tyrosine-protein kinase BTK antibody
      • tyrosine-protein kinase BTK isoform (lacking exon 14 antibody
      • XLA antibody
      see all

    Images

    • Western blot - Anti-BTK antibody [Y440] (ab32555)
      Western blot - Anti-BTK antibody [Y440] (ab32555)
      All lanes : Anti-BTK antibody [Y440] (ab32555) at 1/1000 dilution (Purified)

      Lane 1 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate
      Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate
      Lane 3 : U-937 (Human histiocytic lymphoma monocyte) whole cell lysate

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 76 kDa
      Observed band size: 75 kDa why is the actual band size different from the predicted?

    • Immunoprecipitation - Anti-BTK antibody [Y440] (ab32555)
      Immunoprecipitation - Anti-BTK antibody [Y440] (ab32555)

      BTK was immunoprecipitated from 0.35 mg Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate 10 µg with ab32555 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate 10 µg

      Lane 2: ab32555 IP in Daudi whole cell lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab32555 in Daudi whole cell lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

       

    • Anti-BTK antibody [Y440] (ab32555)
      Anti-BTK antibody [Y440] (ab32555)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab32555? Please let us know so that we can cite the reference in this datasheet.

    ab32555 has been referenced in 1 publication.

    • Alflen A  et al. Idelalisib impairs TREM-1 mediated neutrophil inflammatory responses. Sci Rep 8:5558 (2018). PubMed: 29615799

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-BTK antibody [Y440]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (B lymphoma cells)
    Loading amount
    100 µg
    Specification
    B lymphoma cells
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 29 2010

    Question

    Hello,

    I am interested in Anti-BTK antibody (ab32555) for immunoprecipitation. I was wondering if you have data which shows that this antibody works for IP, or not. Thank you for your help,

    Read More

    Abcam community

    Verified customer

    Asked on Jul 25 2012

    Answer

    Thank you very much for contacting us with your questions, and for your patience while I have been in touch with the lab regarding your enquiry.
    I can confirm that ab32555 works in IP, but unfortunately we do not have any data to forward to you. The antibody was used at a 1:20 dilution and found to work. We fully guarantee the antibody in IP with human samples, so if you did have any trouble with it we would be happy to send a replacement or issue a credit or refund.


    I hope that this information will be useful, but please let me know if you have any further questions and we'll be happy to help.

    Read More

    Abcam Scientific Support

    Answered on Jul 25 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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