Recombinant Anti-BTK antibody [Y440] (ab32555)
![Western blot - Anti-BTK antibody [Y440] (ab32555)](https://www.abcam.com/ps/products/32/ab32555/Images/ab32555-478313-anti-btk-antibody-y440-westernblot-human.jpg "Western blot - Anti-BTK antibody [Y440] (ab32555)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [Y440] to BTK
- Suitable for: WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-BTK antibody [Y440]
See all BTK primary antibodies -
Description
Rabbit monoclonal [Y440] to BTK -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human BTK aa 600-700 (C terminal). The exact sequence is proprietary.
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Positive control
- WB: Daudi cell lysate, K-562 cell lysate, U-937 cell lysate.
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General notes
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Y440 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab32555 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000. Predicted molecular weight: 76 kDa.
|
| IP |
1/30.
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| Notes |
|---|
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WB
1/1000. Predicted molecular weight: 76 kDa. |
|
IP
1/30. |
Target
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Function
Plays a crucial role in B-cell ontogeny. Transiently phosphorylates GTF2I on tyrosine residues in response to B-cell receptor cross-linking. Required for the formation of functional ARID3A DNA-binding complexes. -
Involvement in disease
Defects in BTK are the cause of X-linked agammaglobulinemia (XLA) [MIM:300755]; also known as X-linked agammaglobulinemia type 1 (AGMX1) or immunodeficiency type 1 (IMD1). XLA is a humoral immunodeficiency disease which results in developmental defects in the maturation pathway of B-cells. Affected boys have normal levels of pre-B-cells in their bone marrow but virtually no circulating mature B-lymphocytes. This results in a lack of immunoglobulins of all classes and leads to recurrent bacterial infections like otitis, conjunctivitis, dermatitis, sinusitis in the first few years of life, or even some patients present overwhelming sepsis or meningitis, resulting in death in a few hours. Treatment in most cases is by infusion of intravenous immunoglobulin.
Defects in BTK may be the cause of X-linked hypogammaglobulinemia and isolated growth hormone deficiency (XLA-IGHD) [MIM:307200]; also known as agammaglobulinemia and isolated growth hormone deficiency or Fleisher syndrome or isolated growth hormone deficiency type 3 (IGHD3). In rare cases XLA is inherited together with isolated growth hormone deficiency (IGHD). -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
Contains 1 Btk-type zinc finger.
Contains 1 PH domain.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
Post-translational
modificationsAutophosphorylated on Tyr-223 and Tyr-551. Phosphorylation of Tyr-223 may create a docking site for a SH2 containing protein. -
Cellular localization
Cytoplasm. Membrane. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 695 Human
- Omim: 300300 Human
- SwissProt: Q06187 Human
- Unigene: 159494 Human
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Alternative names
- Agammaglobulinaemia tyrosine kinase antibody
- AGMX 1 antibody
- AGMX1 antibody
see all
Images
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Western blot - Anti-BTK antibody [Y440] (ab32555)All lanes : Anti-BTK antibody [Y440] (ab32555) at 1/1000 dilution (Purified)
Lane 1 : Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate
Lane 2 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate
Lane 3 : U-937 (Human histiocytic lymphoma monocyte) whole cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 76 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted? -
![Immunoprecipitation - Anti-BTK antibody [Y440] (ab32555)](https://www.abcam.com/ps/products/32/ab32555/Images/ab32555-3-anti-btk-antibody-y440-immunoprecipitation-daudi-human.jpg)
Immunoprecipitation - Anti-BTK antibody [Y440] (ab32555)BTK was immunoprecipitated from 0.35 mg Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate 10 µg with ab32555 at 1/50 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Daudi (Human Burkitt's lymphoma lymphoblast) whole cell lysate 10 µg
Lane 2: ab32555 IP in Daudi whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab32555 in Daudi whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
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![Anti-BTK antibody [Y440] (ab32555)](https://www.abcam.com/ps/products/32/ab32555/Images/ab32555-2-benefits-of-recombinant-antibodies.png)
Anti-BTK antibody [Y440] (ab32555)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab32555 has been referenced in 1 publication.
- Alflen A et al. Idelalisib impairs TREM-1 mediated neutrophil inflammatory responses. Sci Rep 8:5558 (2018). PubMed: 29615799