Recombinant Anti-c-Jun antibody [3/Jun] (ab280089)
![Western blot - Anti-c-Jun antibody [3/Jun] (ab280089)](https://www.abcam.com/ps/products/280/ab280089/Images/ab280089-455105-anti-cjun-antibody-3jun-western-blot-hek293.jpg "Western blot - Anti-c-Jun antibody [3/Jun] (ab280089)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [3/Jun] to c-Jun
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-c-Jun antibody [3/Jun]
See all c-Jun primary antibodies -
Description
Mouse monoclonal [3/Jun] to c-Jun -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293, HeLa, NIH/3T3. PC-12 and MDA-MB-231 whole cell lysate. IHC-P: Human esophagus and colon tissue. Mouse and rat hippocampus tissue. Mouse and rat cerebrum tissue. ICC/IF: MDA-MB-231 cells.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
3/Jun -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry reagents
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab280089 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
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| WB |
1/1000. Predicted molecular weight: 35 kDa.
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| ICC/IF |
1/50.
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| Notes |
|---|
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IHC-P
1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
1/1000. Predicted molecular weight: 35 kDa. |
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ICC/IF
1/50. |
Target
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Function
Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306). -
Sequence similarities
Belongs to the bZIP family. Jun subfamily.
Contains 1 bZIP (basic-leucine zipper) domain. -
Post-translational
modificationsUbiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7.
Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity.
Acetylated at Lys-271 by EP300. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3725 Human
- Entrez Gene: 16476 Mouse
- Entrez Gene: 24516 Rat
- Omim: 165160 Human
- SwissProt: P05412 Human
- SwissProt: P05627 Mouse
- SwissProt: P17325 Rat
- Unigene: 696684 Human
see all -
Alternative names
- Activator protein 1 antibody
- AP 1 antibody
- AP-1 antibody
see all
Images
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Western blot - Anti-c-Jun antibody [3/Jun] (ab280089)All lanes : Anti-c-Jun antibody [3/Jun] (ab280089) at 1/1000 dilution
Lane 1 : HEK-293 (human embryonic kidney epithelial cell), whole cell lysate
Lane 2 : T-47D (human ductal breast epithelial tumor epithelial cell), whole cell lysate
Lane 3 : MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 35 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 20976196).
Negative control: T-47D (PMID: 24830720).
Exposure time: lane 1: 3 minutes
lane 2, 3:15 seconds
lane 4: 37 seconds -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody [3/Jun] (ab280089)](https://www.abcam.com/ps/products/280/ab280089/Images/ab280089-1-anti-cjun-antibody-3jun-immunohistochemistry-human-esophagus.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody [3/Jun] (ab280089)Immunohistochemical analysis of paraffin-embedded human esophagus tissue labeling c-Jun with ab280089 at 1/5000 (0.196 µg/ml) dilution followed by a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on human esophagus.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).
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![Immunocytochemistry/ Immunofluorescence - Anti-c-Jun antibody [3/Jun] (ab280089)](https://www.abcam.com/ps/products/280/ab280089/Images/ab280089-7-anti-cjun-antibody-3jun-immunocytochemistry-mdamb231.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-c-Jun antibody [3/Jun] (ab280089)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MDA-MB-231 cells labelling c-Jun with ab280089 at 1/50 (19.56 µg/ml) dilution, followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green).
Confocal image showing nuclear staining in MDA-MB-231 cells.
Negative control: MCF7 (PMID: 24830720).
ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Negative control 1: ab280089 at a 1/50 dilution followed by ab150080 at a 1/500 dilution.
Negative control 2: ab179513 at a 1/200 dilution followed by ab150113 at a 1/1000 dilution.
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![Western blot - Anti-c-Jun antibody [3/Jun] (ab280089)](https://www.abcam.com/ps/products/280/ab280089/Images/ab280089-455106-anti-cjun-antibody-3jun-western-blot-hela.jpg)
Western blot - Anti-c-Jun antibody [3/Jun] (ab280089)All lanes : Anti-c-Jun antibody [3/Jun] (ab280089) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell ), whole cell lysate
Lane 2 : HEK-293 (human embryonic kidney epithelial cell), whole cell lysate
Lane 3 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lane 4 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution
Predicted band size: 35 kDaLysates were made freshly and used in WB immediately to minimize protein degradation.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: lane 1: 3 mins;
lane 2, 3: 15 secs;
lane 4: 37 secs.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody [3/Jun] (ab280089)](https://www.abcam.com/ps/products/280/ab280089/Images/ab280089-2-anti-cjun-antibody-3jun-immunohistochemistry-human-colon.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody [3/Jun] (ab280089)Immunohistochemical analysis of paraffin-embedded human colon tissue labeling c-Jun with ab280089 at 1/5000 (0.196 µg/ml) dilution followed by a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Scattered nuclear staining on human colon.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody [3/Jun] (ab280089)](https://www.abcam.com/ps/products/280/ab280089/Images/ab280089-3-anti-cjun-antibody-3jun-immunohistochemistry-mouse-hippocampus.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody [3/Jun] (ab280089)Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling c-Jun with ab280089 at 1/5000 (0.196 µg/ml) dilution followed by a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on the dentate gyrus of mouse hippocampus.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody [3/Jun] (ab280089)](https://www.abcam.com/ps/products/280/ab280089/Images/ab280089-4-anti-cjun-antibody-3jun-immunohistochemistry-mouse-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody [3/Jun] (ab280089)Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling c-Jun with ab280089 at 1/5000 (0.196 µg/ml) dilution followed by a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on mouse cerebrum.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody [3/Jun] (ab280089)](https://www.abcam.com/ps/products/280/ab280089/Images/ab280089-5-anti-cjun-antibody-3jun-immunohistochemistry-rat-hippocampus.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody [3/Jun] (ab280089)Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling c-Jun with ab280089 at 1/5000 (0.196 µg/ml) dilution followed by a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on the dentate gyrus of rat hippocampus.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody [3/Jun] (ab280089)](https://www.abcam.com/ps/products/280/ab280089/Images/ab280089-6-anti-cjun-antibody-3jun-immunohistochemistry-rat-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Jun antibody [3/Jun] (ab280089)Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling c-Jun with ab280089 at 1/5000 (0.196 µg/ml) dilution followed by a ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Nuclear staining on rat cerebrum.
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).
Heat mediated antigen retrieval using ab93678 (citrate buffer, pH 6.0).
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![Anti-c-Jun antibody [3/Jun] (ab280089)](https://www.abcam.com/ps/products/280/ab280089/Images/ab280089-455156-benefitsofrecombinantantibodies.jpg)
Anti-c-Jun antibody [3/Jun] (ab280089)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab280089 has been referenced in 1 publication.
- Zhang YL et al. Progesterone suppresses the progression of colonic carcinoma by increasing the activity of the GADD45a/JNK/c-Jun signalling pathway. Oncol Rep 45:N/A (2021). PubMed: 33846816