Recombinant Anti-c-Jun (phospho T93) antibody [EPR2237] (ab81319)
![Western blot - Anti-c-Jun (phospho T93) antibody [EPR2237] (ab81319)](https://www.abcam.com/ps/products/81/ab81319/Images/ab81319-96534-anti-c-jun-phospho-t93-antibody-epr2237-western-blot.jpg "Western blot - Anti-c-Jun (phospho T93) antibody [EPR2237] (ab81319)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2237] to c-Jun (phospho T93)
- Suitable for: WB
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-c-Jun (phospho T93) antibody [EPR2237]
See all c-Jun primary antibodies -
Description
Rabbit monoclonal [EPR2237] to c-Jun (phospho T93) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse
Predicted to work with: Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- NIH/3T3 cell lysates treated with Anisomycin
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2237 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab81319 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
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| Notes |
|---|
|
WB
1/1000 - 1/5000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa). |
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
Target
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Function
Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306). -
Sequence similarities
Belongs to the bZIP family. Jun subfamily.
Contains 1 bZIP (basic-leucine zipper) domain. -
Post-translational
modificationsUbiquitinated by the SCF(FBXW7), leading to its degradation. Ubiquitination takes place following phosphorylation, that promotes interaction with FBXW7.
Phosphorylated by CaMK4 and PRKDC; phosphorylation enhances the transcriptional activity. Phosphorylated by HIPK3. Phosphorylated by DYRK2 at Ser-243; this primes the protein for subsequent phosphorylation by GSK3B at Thr-239. Phosphorylated at Thr-239, Ser-243 and Ser-249 by GSK3B; phosphorylation reduces its ability to bind DNA. Phosphorylated by PAK2 at Thr-2, Thr-8, Thr-89, Thr-93 and Thr-286 thereby promoting JUN-mediated cell proliferation and transformation. Phosphorylated by PLK3 following hypoxia or UV irradiation, leading to increase DNA-binding activity.
Acetylated at Lys-271 by EP300. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3725 Human
- Entrez Gene: 16476 Mouse
- Omim: 165160 Human
- SwissProt: P05412 Human
- SwissProt: P05627 Mouse
- Unigene: 696684 Human
- Unigene: 275071 Mouse
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Alternative names
- Activator protein 1 antibody
- AP 1 antibody
- AP-1 antibody
see all
Images
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Western blot - Anti-c-Jun (phospho T93) antibody [EPR2237] (ab81319)All lanes : Anti-c-Jun (phospho T93) antibody [EPR2237] (ab81319) at 1/5000 dilution
Lane 1 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate, untreated
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate, treated with Anisomycin
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP goat anti rabbit at 1/2000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa -
![Anti-c-Jun (phospho T93) antibody [EPR2237] (ab81319)](https://www.abcam.com/ps/products/81/ab81319/Images/ab81319-2-benefits-of-recombinant-antibodies.png)
Anti-c-Jun (phospho T93) antibody [EPR2237] (ab81319)
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab81319 has been referenced in 3 publications.
- Brandolini L et al. Paclitaxel binds and activates C5aR1: A new potential therapeutic target for the prevention of chemotherapy-induced peripheral neuropathy and hypersensitivity reactions. Cell Death Dis 13:500 (2022). PubMed: 35614037
- Sun T et al. Knockdown of Long Non-Coding RNA RP11-445H22.4 Alleviates LPS-Induced Injuries by Regulation of MiR-301a in Osteoarthritis. Cell Physiol Biochem 45:832-843 (2018). PubMed: 29414810
- Jiang R et al. MicroRNA-126 Inhibits Proliferation, Migration, Invasion, and EMT in Osteosarcoma by Targeting ZEB1. J Cell Biochem 118:3765-3774 (2017). PubMed: 28379605