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Immunology Cell Type Markers CD Cytokines
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RecombinantRabMAb

Recombinant Anti-c-Kit antibody [EPR24708-25] (ab273119)

  • Datasheet
  • SDS
  • Certificate of Compliance
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Immunocytochemistry/ Immunofluorescence - Anti-c-Kit antibody [EPR24708-25] (ab273119)
  • Flow Cytometry - Anti-c-Kit antibody (ab273119)
  • Flow Cytometry - Anti-c-Kit antibody (ab273119)
  • Anti-c-Kit antibody (ab273119)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR24708-25] to c-Kit
  • Suitable for: Flow Cyt, ICC/IF
  • Reacts with: Mouse

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free

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Overview

  • Product name

    Anti-c-Kit antibody [EPR24708-25]
    See all c-Kit primary antibodies
  • Description

    Rabbit monoclonal [EPR24708-25] to c-Kit
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P,IP or WB
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: P815 cells. Flow Cyt: Mouse bone marrow cells.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR24708-25
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Cell Type Markers
    • CD
    • Cytokines
    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Signal Transduction
    • Protein Phosphorylation
    • Tyrosine Kinases
    • Receptor Tyrosine Kinases
    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Tyrosine Kinase Receptors
    • Stem Cells
    • Germline Stem Cells
    • Embryonic Germ Cells
    • Stem Cells
    • Hematopoietic Progenitors
    • Surface Molecules
    • Cancer
    • Signal transduction
    • Protein phosphorylation
    • Tyrosine kinases
    • Receptor tyrosine kinases
    • Cancer
    • Oncoproteins/suppressors
    • Oncoproteins
    • Growth factor receptors
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Mast Cell Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • HSC markers
    • Developmental Biology
    • Reproduction
    • Germ cell markers
    • Developmental Biology
    • Organogenesis
    • Hematopoietic system development
    • Neuroscience
    • Development

Associated products

  • Alternative Versions

    • Anti-c-Kit antibody [EPR24708-25] - BSA and Azide free (ab281843)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab273119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
1/500.
ICC/IF
1/50.
Notes
Flow Cyt
1/500.
ICC/IF
1/50.
Application notes
Is unsuitable for IHC-P,IP or WB.

Target

  • Function

    Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.
  • Tissue specificity

    Isoform 1 and isoform 2 are detected in spermatogonia and Leydig cells. Isoform 3 is detected in round spermatids, elongating spermatids and spermatozoa (at protein level). Widely expressed. Detected in the hematopoietic system, the gastrointestinal system, in melanocytes and in germ cells.
  • Involvement in disease

    Piebald trait
    Gastrointestinal stromal tumor
    Testicular germ cell tumor
    Leukemia, acute myelogenous
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
    Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications

    Ubiquitinated by SOCS6. KIT is rapidly ubiquitinated after autophosphorylation induced by KITLG/SCF binding, leading to internalization and degradation.
    Autophosphorylated on tyrosine residues. KITLG/SCF binding enhances autophosphorylation. Isoform 1 shows low levels of tyrosine phosphorylation in the absence of added KITLG/SCF (in vitro). Kinase activity is down-regulated by phosphorylation on serine residues by protein kinase C family members. Phosphorylation at Tyr-568 is required for interaction with PTPN11/SHP-2, CRK (isoform Crk-II) and members of the SRC tyrosine-protein kinase family. Phosphorylation at Tyr-570 is required for interaction with PTPN6/SHP-1. Phosphorylation at Tyr-703, Tyr-823 and Tyr-936 is important for interaction with GRB2. Phosphorylation at Tyr-721 is important for interaction with PIK3R1. Phosphorylation at Tyr-823 and Tyr-936 is important for interaction with GRB7.
  • Cellular localization

    Cell membrane and Cytoplasm. Detected in the cytoplasm of spermatozoa, especially in the equatorial and subacrosomal region of the sperm head.
  • Target information above from: UniProt accession P10721 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 16590 Mouse
    • SwissProt: P05532 Mouse
    • Unigene: 247073 Mouse
    • Alternative names

      • C Kit antibody
      • c-Kit antibody
      • c-Kit Ligand antibody
      • CD117 antibody
      • Kit antibody
      • Kit Ligand antibody
      • KIT oncogene antibody
      • KIT proto oncogene receptor tyrosine kinase antibody
      • KIT_HUMAN antibody
      • Mast cell growth factor receptor antibody
      • Mast/stem cell growth factor receptor Kit antibody
      • MGF antibody
      • p145 c-kit antibody
      • PBT antibody
      • Piebald trait protein antibody
      • Proto oncogene c Kit antibody
      • Proto oncogene tyrosine protein kinase Kit antibody
      • Proto-oncogene c-Kit antibody
      • SCF Receptor antibody
      • SCFR antibody
      • soluble KIT variant 1 antibody
      • Steel Factor Receptor antibody
      • Stem cell factor receptor antibody
      • tyrosine protein kinase Kit antibody
      • Tyrosine-protein kinase Kit antibody
      • v kit Hardy Zuckerman 4 feline sarcoma viral oncogene homolog antibody
      • v kit Hardy Zuckerman 4 feline sarcoma viral oncogene like protein antibody
      • v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-c-Kit antibody [EPR24708-25] (ab273119)
      Immunocytochemistry/ Immunofluorescence - Anti-c-Kit antibody [EPR24708-25] (ab273119)

      Immunofluorescent analysis of fixed, 0.1% TritonX-100 permeabilized P815 cells labelling c-Kit with ab273119 at 1/50 (10.28 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green) Confocal image showing membranous staining in P815 cell line. Negative control: NIH/3T3 (PMID: 10523834) is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

    • Flow Cytometry - Anti-c-Kit antibody (ab273119)
      Flow Cytometry - Anti-c-Kit antibody (ab273119)

      Flow cytometric analysis of Mouse bone marrow cell cells labelling c-Kit with ab273119 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control. Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody. Cells were stained with anti-Lineage Cocktail (CD3+Gr-1+CD11b+CD45R+Ter-119) conjugated to FITC. Gated on viable cells.

    • Flow Cytometry - Anti-c-Kit antibody (ab273119)
      Flow Cytometry - Anti-c-Kit antibody (ab273119)

      Flow cytometric analysis of Mouse bone marrow cell cells labelling c-Kit with ab273119 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control. Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody. Cells were stained with anti-CD34 conjugated to BV421. Gated on viable cells.

    • Anti-c-Kit antibody (ab273119)
      Anti-c-Kit antibody (ab273119)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab273119? Please let us know so that we can cite the reference in this datasheet.

    ab273119 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunocytochemistry/ Immunofluorescence abreview for Anti-c-Kit antibody [EPR24708-25]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Testis)
    Permeabilization
    Yes - 0.25% Triton X-100 for 5 minutes
    Specification
    Testis
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Brad Hansen

    Verified customer

    Submitted Oct 10 2022

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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