Recombinant Anti-C10 antibody [EPR14614] (ab191400)
![Western blot - Anti-C10 antibody [EPR14614] (ab191400)](https://www.abcam.com/ps/products/191/ab191400/Images/ab191400-228658-anti-c10-antibody-epr14614-western-blot.jpg "Western blot - Anti-C10 antibody [EPR14614] (ab191400)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14614] to C10
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-C10 antibody [EPR14614]
See all C10 primary antibodies -
Description
Rabbit monoclonal [EPR14614] to C10 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Recombinant mouse C10 protein, mouse and rat spleen tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14614 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab191400 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).
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| IHC-P |
1/1000 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa). |
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IHC-P
1/1000 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Relevance
CCL6 (C10) is a small cytokine belonging to the CC chemokine family that has only been identified in rodents. It is a potent chemoattractant of macrophages, but it can also attract B cells, CD4+ lymphocytes and eosinophils. In mice, CCL6 is expressed in cells from neutrophil and macrophage lineages, and can be induced under conditions suitable for myeloid cell differentiation. CCL6 (C10) was originally identified as a transcript that is induced in bone marrow cells upon stimulation of GMCSF. The cell surface receptor for CCL6 is believed to be the chemokine receptor CCR1. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 20305 Mouse
- Entrez Gene: 287910 Rat
- SwissProt: P27784 Mouse
- SwissProt: Q68FP3 Rat
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Alternative names
- C10 antibody
- CCL 6 antibody
- chemokine (C-C motif) ligand 6 antibody
see all
Images
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Western blot - Anti-C10 antibody [EPR14614] (ab191400)Anti-C10 antibody [EPR14614] (ab191400) at 1/10000 dilution + Recombinant mouse C10 protein at 20 µg
Secondary
goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 13 kDa
Observed band size: 13 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10 antibody [EPR14614] (ab191400)](https://www.abcam.com/ps/products/191/ab191400/Images/ab191400-228660-anti-c10-antibody-epr14614-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10 antibody [EPR14614] (ab191400)Immunohistochemical analysis of paraffin-embedded, mouse spleen tissue labeling C10 with ab191400 at a 1/2000 dilution (0.6μg/ml). Counter stained with hematoxylin. In the negative control PBS was used instead of primary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10 antibody [EPR14614] (ab191400)](https://www.abcam.com/ps/products/191/ab191400/Images/ab191400-228659-anti-c10-antibody-epr14614-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C10 antibody [EPR14614] (ab191400)Immunohistochemical analysis of paraffin-embedded, rat spleen tissue labeling C10 with ab191400 at a 1/2000 dilution (0.6μg/ml). Counter stained with hematoxylin. In the negative control PBS was used instead of primary antibody.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Anti-C10 antibody [EPR14614] (ab191400)](https://www.abcam.com/ps/products/191/ab191400/Images/ab191400-4-benefits-of-recombinant-antibodies.png)
Anti-C10 antibody [EPR14614] (ab191400)
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (1)
ab191400 has been referenced in 1 publication.
- Du X et al. Eosinophil-derived chemokine (hCCL15/23, mCCL6) interacts with CCR1 to promote eosinophilic airway inflammation. Signal Transduct Target Ther 6:91 (2021). PubMed: 33640900
