Anti-C13orf31 antibody (ab254846)
Key features and details
- Rabbit polyclonal to C13orf31
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-C13orf31 antibody -
Description
Rabbit polyclonal to C13orf31 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human C13orf31 aa 1-150.
Database link: Q8IV20 -
Positive control
- IHC-P: Human testis tissue. WB: C13orf31 overexpression HEK-293T cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab254846 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 43 kDa.
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| IHC-P |
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
|
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 43 kDa. |
|
IHC-P
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Sequence similarities
Belongs to the UPF0124 family. - Information by UniProt
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Database links
- Entrez Gene: 144811 Human
- SwissProt: Q8IV20 Human
- Unigene: 210586 Human
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Alternative names
- C13orf31 antibody
- Chromosome 13 open reading frame 31 antibody
- CM031_HUMAN antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C13orf31 antibody (ab254846)Paraffin-embedded human testis tissue stained for C13orf31 using ab254846 at 1/20 dilution in immunohistochemical analysis.
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Western blot - Anti-C13orf31 antibody (ab254846)All lanes : Anti-C13orf31 antibody (ab254846) at 0.4 µg/ml
Lane 1 : Vector only transfected HEK-293T cell lysate
Lane 2 : C13orf31 overexpressed HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Predicted band size: 43 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab254846 has not yet been referenced specifically in any publications.