Recombinant Anti-C1q antibody [4.8] (ab182451)
![Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-C1q antibody [4.8] (ab182451)](https://www.abcam.com/ps/products/182/ab182451/Images/ab182451-390839-anti-c1q-antibody-48-immunohistochemistry-frozen-ko-brain-mouse.jpg "Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-C1q antibody [4.8] (ab182451)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [4.8] to C1q
- Suitable for: IHC-FoFr
- Knockout validated
- Reacts with: Mouse
Overview
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Product name
Anti-C1q antibody [4.8]
See all C1q primary antibodies -
Description
Rabbit monoclonal [4.8] to C1q -
Host species
Rabbit -
Tested applications
Suitable for: IHC-FoFrmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Full length native protein (purified) corresponding to Mouse C1q.
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Positive control
- IHC: Mouse brain tissue (adult) .
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General notes
This antibody was developed as part of a collaboration between Abcam and the lab of Ben A. Barres at Stanford University:
Alexander H. Stephan et al. , A Dramatic Increase of C1q Protein in the CNS during Normal Aging ,The Journal of Neuroscience.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
4.8 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab182451 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-FoFr | (1) |
Use a concentration of 1 µg/ml.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
IHC-FoFr
Use a concentration of 1 µg/ml. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes. -
Involvement in disease
Defects in C1QA are a cause of complement component C1q deficiency (C1QD) [MIM:613652]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis. -
Sequence similarities
Contains 1 C1q domain.
Contains 1 collagen-like domain. -
Post-translational
modificationsO-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 12259 Mouse
- Entrez Gene: 12260 Mouse
- Entrez Gene: 12262 Mouse
- SwissProt: P14106 Mouse
- SwissProt: P98086 Mouse
- SwissProt: Q02105 Mouse
- Unigene: 439957 Mouse
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Alternative names
- C1qa antibody
- C1QA_HUMAN antibody
- C1QB antibody
see all
Images
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-C1q antibody [4.8] (ab182451)This image is kindly provided by Dr. Daniel Wilton from Boston Children’s Hospital.
ab182451 (1/500) staining C1q in 15µm coronal brain sections from postnatal day 90 C1qA KO mice and WT litter-mates (Botto et al., 1998) by immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde and permeabilized with 0.3% Triton. An Alexa Flour-conjugated Goat anti-Mouse IgG polyclonal (1/500) was used as the secondary antibody. Positive staining on wild type (WT) mouse cerebral cortex, and no staining on knockout (KO) mouse cerebral cortex.
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![Anti-C1q antibody [4.8] (ab182451)](https://www.abcam.com/ps/products/182/ab182451/Images/ab182451-4-benefits-of-recombinant-antibodies.png)
Anti-C1q antibody [4.8] (ab182451)
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (34)
ab182451 has been referenced in 34 publications.
- Fairley LH et al. Neuroprotective effect of mitochondrial translocator protein ligand in a mouse model of tauopathy. J Neuroinflammation 18:76 (2021). PubMed: 33740987
- Salvany S et al. Microglial recruitment and mechanisms involved in the disruption of afferent synaptic terminals on spinal cord motor neurons after acute peripheral nerve injury. Glia 69:1216-1240 (2021). PubMed: 33386754
- Frew J & Nygaard HB Neuropathological and behavioral characterization of aged Grn R493X progranulin-deficient frontotemporal dementia knockin mice. Acta Neuropathol Commun 9:57 (2021). PubMed: 33795008
- Clayton K et al. Plaque associated microglia hyper-secrete extracellular vesicles and accelerate tau propagation in a humanized APP mouse model. Mol Neurodegener 16:18 (2021). PubMed: 33752701
- Xiong Z et al. Platelet-Derived Growth Factor-D Activates Complement System to Propagate Macrophage Polarization and Neovascularization. Front Cell Dev Biol 9:686886 (2021). PubMed: 34150781
