Recombinant Anti-C2 antibody [EPR17979] (ab209900)
![Western blot - Anti-C2 antibody [EPR17979] (ab209900)](https://www.abcam.com/ps/products/209/ab209900/Images/ab209900-257656-anti-c2-antibody-epr17979-western-blot.jpg "Western blot - Anti-C2 antibody [EPR17979] (ab209900)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17979] to C2
- Suitable for: WB
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-C2 antibody [EPR17979]
See all C2 primary antibodies -
Description
Rabbit monoclonal [EPR17979] to C2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human serum, plasma and fetal kidney lysate; Mouse serum.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17979 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab209900 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 83 kDa).
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| Notes |
|---|
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WB
1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 83 kDa). |
Target
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Function
Component C2 which is part of the classical pathway of the complement system is cleaved by activated factor C1 into two fragments: C2b and C2a. C2a, a serine protease, then combines with complement factor 4b to generate the C3 or C5 convertase. -
Involvement in disease
Defects in C2 are the cause of complement component 2 deficiency (C2D) [MIM:217000]. A deficiency of the complement classical pathway associated with the development of autoimmune disorders, mainly systemic lupus erythematosus. Skin and joint manifestations are common and renal disease is relatively rare. Patients with complement component 2 deficiency are also reported to have recurrent or invasive infections. -
Sequence similarities
Belongs to the peptidase S1 family.
Contains 1 peptidase S1 domain.
Contains 3 Sushi (CCP/SCR) domains.
Contains 1 VWFA domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 717 Human
- Entrez Gene: 12263 Mouse
- Omim: 217000 Human
- SwissProt: P06681 Human
- SwissProt: P21180 Mouse
- Unigene: 408903 Human
- Unigene: 283217 Mouse
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Alternative names
- ARMD14 antibody
- C2 antibody
- C3/C5 convertase antibody
see all
Images
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Western blot - Anti-C2 antibody [EPR17979] (ab209900)All lanes : Anti-C2 antibody [EPR17979] (ab209900) at 1/1000 dilution
Lane 1 : Human serum
Lane 2 : Human plasma
Lane 3 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lanes 1-2 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Lane 3 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 83 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1/3: 3 minutes; Lane 2: 30 seconds.
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![Western blot - Anti-C2 antibody [EPR17979] (ab209900)](https://www.abcam.com/ps/products/209/ab209900/Images/ab209900-257655-anti-c2-antibody-epr17979-western-blot.jpg)
Western blot - Anti-C2 antibody [EPR17979] (ab209900)Anti-C2 antibody [EPR17979] (ab209900) at 1/1000 dilution + Mouse serum at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 83 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocking/Dilution buffer: 5% NFDM/TBST.
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![Anti-C2 antibody [EPR17979] (ab209900)](https://www.abcam.com/ps/products/209/ab209900/Images/ab209900-3-benefits-of-recombinant-antibodies.png)
Anti-C2 antibody [EPR17979] (ab209900)
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab209900 has been referenced in 1 publication.
- Pei J et al. Differential proteomic analysis demonstrates follicle fluid participate immune reaction and protein translation in yak. BMC Vet Res 18:34 (2022). PubMed: 35031034