Name: Anti-C3 antibody [EPR19394]
Brand: RabMAb
SKU: ab200999
Rating: 4 (9 reviews)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

Loading size & price…

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Western blot - Anti-C3 antibody [EPR19394] (ab200999)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR19394] to C3
Suitable for: IHC-P, WB
Reacts with: Mouse, Rat, Human

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free Carrier Free

Product name

Anti-C3 antibody [EPR19394]
See all C3/C3b primary antibodies
Description

Rabbit monoclonal [EPR19394] to C3
Host species

Rabbit
Tested applications

Suitable for: IHC-P, WBmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human fetal liver, fetal heart and fetal kidney lysates; Mouse brain, kidney, spleen and heart lysates; Rat brain, spleen, heart and kidney lysates; Human serum, plasma and milk; HepG2 whole cell lysate; Mouse and rat plasma. IHC-P: Mouse liver, cerebral cortex and cardiac muscle tissues; Rat kidney, liver and lung tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR19394
Isotype

IgG
Research areas

Alternative Versions
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human C3 protein (ab157985)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab200999 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IHC-P	(4)	1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB	(2)	1/2000. Detects a band of approximately 187, 115 ,68 ,43 kDa (predicted molecular weight: 187 kDa).

Notes
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/2000. Detects a band of approximately 187, 115 ,68 ,43 kDa (predicted molecular weight: 187 kDa).

Function

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
Tissue specificity

Plasma.
Involvement in disease

Defects in C3 are the cause of complement component 3 deficiency (C3D) [MIM:120700]. A rare defect of the complement classical pathway. Patients develop recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Some patients may also develop autoimmune disorders, such as arthralgia and vasculitic rashes, lupus-like syndrome and membranoproliferative glomerulonephritis.
Genetic variation in C3 is associated with susceptibility to age-related macular degeneration type 9 (ARMD9) [MIM:611378]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
Defects in C3 are a cause of susceptibility to hemolytic uremic syndrome atypical type 5 (AHUS5) [MIM:612925]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
Sequence similarities

Contains 1 anaphylatoxin-like domain.
Contains 1 NTR domain.
Post-translational
modifications

C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g.
Phosphorylation sites are present in the extracelllular medium.
Cellular localization

Secreted.
Target information above from: UniProt accession P01024 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 718 Human
- Entrez Gene: 12266 Mouse
- Entrez Gene: 24232 Rat
- Omim: 120700 Human
- SwissProt: P01024 Human
- SwissProt: P01027 Mouse
- SwissProt: P01026 Rat
- Unigene: 529053 Human
- Unigene: 19131 Mouse
- Unigene: 11378 Rat
see all
Alternative names
- Acylation stimulating protein cleavage product antibody
- AHUS5 antibody
- ARMD9 antibody
- ASP antibody
- C3 and PZP like alpha 2 macroglobulin domain containing protein 1 antibody
- C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1 antibody
- C3 antibody
- c3 complement antibody
- C3adesArg antibody
- CO3_HUMAN antibody
- Complement C3 alpha chain antibody
- Complement C3 antibody
- Complement C3b alpha' chain antibody
- Complement C3c alpha' chain fragment 1 antibody
- Complement C3c alpha' chain fragment 2 antibody
- Complement C3c alpha'' chain fragment 2 antibody
- Complement C3d fragment antibody
- Complement C3dg fragment antibody
- Complement C3f fragment antibody
- Complement C3g fragment antibody
- Complement component 3 antibody
- Complement factor 3 antibody
- CPAMD1 antibody
- HEL S 62p antibody
- omplement C3 beta chain antibody
see all

Western blot - Anti-C3 antibody [EPR19394] (ab200999)

All lanes : Anti-C3 antibody [EPR19394] (ab200999) at 1/2000 dilution

Lane 1 : Human fetal liver lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 187 kDa
Observed band size: 115,187,68 kDa why is the actual band size different from the predicted?

Exposure time: 30 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3 antibody [EPR19394] (ab200999)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling C3 with ab200999 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasm staining on hepatocytes of mouse liver is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-C3 antibody [EPR19394] (ab200999)

All lanes : Anti-C3 antibody [EPR19394] (ab200999) at 1/2000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse spleen lysate
Lane 4 : Mouse heart lysate
Lane 5 : Rat brain lysate
Lane 6 : Rat spleen lysate
Lane 7 : Rat heart lysate
Lane 8 : Rat kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 187 kDa
Observed band size: 115,187,43,68 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1,2,3,5 and 6: 3 minutes; Lane 4,7 and 8: 10 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3 antibody [EPR19394] (ab200999)

Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling C3 with ab200999 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

The plasma in blood vessel of mouse cardiac muscle was positive staining. The staining pattern is similar to what has been observed in the literature (PMID: 23104558).

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3 antibody [EPR19394] (ab200999)

Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling C3 with ab200999 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

The plasma in blood vessels of mouse cerebral cortex was positive staining.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-C3 antibody [EPR19394] (ab200999)

All lanes : Anti-C3 antibody [EPR19394] (ab200999) at 1/2000 dilution

Lane 1 : Human serum
Lane 2 : Human plasma
Lane 3 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 4 : Mouse plasma
Lane 5 : Rat plasma
Lane 6 : Human milk

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 187 kDa
Observed band size: 115,187,43,68 kDa why is the actual band size different from the predicted?

Blocking/Dilution buffer: 5% NFDM/TBST.

Exposure time: Lane 1-5: 8 seconds; Lane 6: 3 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3 antibody [EPR19394] (ab200999)

Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling C3 with ab200999 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Cytoplasm staining on hepatocytes of rat liver is observed.

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3 antibody [EPR19394] (ab200999)

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling C3 with ab200999 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

The plasma in blood vessels of rat kidney was positive staining. The staining pattern is similar to what has been observed in the literature (PMID: 23104558).

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C3 antibody [EPR19394] (ab200999)

Immunohistochemical analysis of paraffin-embedded Rat lung tissue labeling C3 with ab200999 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

The plasma in blood vessels of rat lung was positive staining. The staining pattern is similar to what has been observed in the literature (PMID: 23104558).

Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-C3 antibody [EPR19394] (ab200999)

Western blot protocols
Immunohistochemistry protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab200999? Please let us know so that we can cite the reference in this datasheet.

ab200999 has been referenced in 47 publications.

Xie T et al. Insight into the Neuroprotective Effect of Genistein-3'-Sodium Sulfonate Against Neonatal Hypoxic-Ischaemic Brain Injury in Rats by Bioinformatics. Mol Neurobiol 60:807-819 (2023). PubMed: 36370154
Choi YJ et al. Promotion of the inflammatory response in mid colon of complement component 3 knockout mice. Sci Rep 12:1700 (2022). PubMed: 35105928
Meng S et al. Age of Rats Affects the Degree of Retinal Neuroinflammatory Response Induced by High Acute Intraocular Pressure. Dis Markers 2022:9404977 (2022). PubMed: 35132339
Wang CW et al. A Weakly Supervised Deep Learning Method for Guiding Ovarian Cancer Treatment and Identifying an Effective Biomarker. Cancers (Basel) 14:N/A (2022). PubMed: 35406422
Torp MK et al. Intracellular Complement Component 3 Attenuated Ischemia-Reperfusion Injury in the Isolated Buffer-Perfused Mouse Heart and Is Associated With Improved Metabolic Homeostasis. Front Immunol 13:870811 (2022). PubMed: 35432387

View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A

Submit a review Submit a question

1-9 of 9 Abreviews or Q&A

Application

Immunohistochemistry (PFA perfusion fixed frozen sections)

Sample

Mouse Tissue sections (Brain)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris EDTA

Specification

Brain

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10 · Temperature: 21°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 31 2022

Application

Western blot

Sample

Mouse Serum (serum)

Gel Running Conditions

Reduced Denaturing (10)

Loading amount

0.25 µg

Specification

serum

Blocking step

Protein-Free (PBS) Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 21°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 29 2022

Application

Western blot

Sample

Human Serum (serum)

Gel Running Conditions

Reduced Denaturing (10% polyacrylamide)

Loading amount

0.25 µg

Specification

serum

Blocking step

Thermo Scientific Ref 37572 as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 21°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 29 2022

Application

Immunohistochemistry (Frozen sections)

Sample

Mouse Tissue sections (Liver from APP mouse and APP C3 knock out mouse)

Permeabilization

Specification

Liver from APP mouse and APP C3 knock out mouse

Blocking step

(agent) for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 26 2022

Application

Flow Cytometry

Sample

Mouse Cell (Bacteria cells co-cultured with mouse serum)

Permeabilization

Gating Strategy

GFP positive bacteria

Specification

Bacteria cells co-cultured with mouse serum

Preparation

Cell harvesting/tissue preparation method: GFP-labeled bacteria was co-cultured with wild-type mouse serum or C3-deficient mouse serum for 1 hour, then stain
Sample buffer: 2% BSA PBS

Fixation

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Meng Wu

Verified customer

Submitted May 24 2021

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (palatine tonsil)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0

Permeabilization

Specification

palatine tonsil

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 03 2020

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (brain)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0

Permeabilization

Specification

brain

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 03 2020

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Monkey Tissue sections (brain)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris/EDTA

Permeabilization

Specification

brain

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 23°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MS. Linh Hellmers

Verified customer

Submitted May 19 2020

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (brain tissue)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris/EDTA ph 9

Specification

brain tissue

Blocking step

(agent) for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 26°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

HERR DR. Markus Kipp

Verified customer

Submitted Aug 21 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Switch on our new BETA site

Now available

In the coming months

Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Involvement in disease

Sequence similarities

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (47)

Customer reviews and Q&As

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-C3 antibody [EPR19394]

Western blot abreview for Anti-C3 antibody [EPR19394]

Western blot abreview for Anti-C3 antibody [EPR19394]

Immunohistochemistry (Frozen sections) abreview for Anti-C3 antibody [EPR19394]

Flow Cytometry abreview for Anti-C3 antibody [EPR19394]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-C3 antibody [EPR19394]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-C3 antibody [EPR19394]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-C3 antibody [EPR19394]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-C3 antibody [EPR19394]

Post-translational
modifications