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    products/primary-antibodies/c3d-antibody-7c10-ab17453.pdf

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Immunology Innate Immunity Complement Classical Pathway
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Anti-C3d antibody [7C10] (ab17453)

  • Datasheet
  • SDS
Reviews (2)Q&A (2)References (11)

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Immunohistochemistry - Anti-C3d antibody [7C10] (ab17453)
  • Immunofluorescence - Anti-C3d antibody [7C10] (ab17453)

Key features and details

  • Mouse monoclonal [7C10] to C3d
  • Suitable for: IHC
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-C3d antibody [7C10]
    See all C3d primary antibodies
  • Description

    Mouse monoclonal [7C10] to C3d
  • Host species

    Mouse
  • Specificity

    This product is specific to C3d, but also C3b and iC3b, since C3d is a product from C3b.

  • Tested applications

    Suitable for: IHCmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length native protein (purified) corresponding to Human C3d.

  • Epitope

    Epitope specificity differs from that of ab17455.
  • Positive control

    • IHC-P: Human kidney tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.098% Sodium azide
    Constituents: PBS, 2.9% Sodium chloride
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    7C10
  • Myeloma

    x63-Ag8.653
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Immunology
    • Innate Immunity
    • Complement
    • Classical Pathway
    • Immunology
    • Innate Immunity
    • Complement
    • Alternative Pathway

Associated products

  • Alternative Versions

    • Anti-C3b / iC3b antibody [7C12] (ab231078)
    • Anti-C3b / iC3b antibody [1H8] (ab231080)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab17453 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IF
1/10.
IHC
1/10.
Notes
IF
1/10.
IHC
1/10.

Target

  • Function

    C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
    Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
  • Tissue specificity

    Plasma.
  • Involvement in disease

    Defects in C3 are the cause of complement component 3 deficiency (C3D) [MIM:613779]. A rare defect of the complement classical pathway. Patients develop recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Some patients may also develop autoimmune disorders, such as arthralgia and vasculitic rashes, lupus-like syndrome and membranoproliferative glomerulonephritis.
    Genetic variation in C3 is associated with susceptibility to age-related macular degeneration type 9 (ARMD9) [MIM:611378]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
    Defects in C3 are a cause of susceptibility to hemolytic uremic syndrome atypical type 5 (AHUS5) [MIM:612925]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype.
  • Sequence similarities

    Contains 1 anaphylatoxin-like domain.
    Contains 1 NTR domain.
  • Post-translational
    modifications

    C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g.
    Phosphorylation sites are present in the extracelllular medium.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P01024 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 718 Human
    • Omim: 120700 Human
    • SwissProt: P01024 Human
    • Unigene: 529053 Human
    • Alternative names

      • Acylation stimulating protein cleavage product antibody
      • AHUS5 antibody
      • ARMD9 antibody
      • ASP antibody
      • C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1 antibody
      • C3 antibody
      • c3 complement antibody
      • CO3_HUMAN antibody
      • Complement C3 antibody
      • Complement C3c alpha' chain fragment 2 antibody
      • Complement C3d fragment antibody
      • Complement component 3 antibody
      • Complement component C3 antibody
      • CPAMD1 antibody
      see all

    Images

    • Immunohistochemistry - Anti-C3d antibody [7C10] (ab17453)
      Immunohistochemistry - Anti-C3d antibody [7C10] (ab17453)

      Immunohistochemical analysis of human kidney labelling C3d with ab17453 at a dilution of 1/10. Plasma of veins were stained strongly.

    • Immunofluorescence - Anti-C3d antibody [7C10] (ab17453)
      Immunofluorescence - Anti-C3d antibody [7C10] (ab17453)

      Immunofluorescent analysis of human kidney labelling C3d with ab17453 at a dilution of 1/10. Plasma of veins were stained strongly.

    Protocols

    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (11)

    Publishing research using ab17453? Please let us know so that we can cite the reference in this datasheet.

    ab17453 has been referenced in 11 publications.

    • Werneburg S  et al. Targeted Complement Inhibition at Synapses Prevents Microglial Synaptic Engulfment and Synapse Loss in Demyelinating Disease. Immunity 52:167-182.e7 (2020). PubMed: 31883839
    • Petrosyan A  et al. A glomerulus-on-a-chip to recapitulate the human glomerular filtration barrier. Nat Commun 10:3656 (2019). PubMed: 31409793
    • Battin C  et al. Neuropilin-1 Acts as a Receptor for Complement Split Products. Front Immunol 10:2209 (2019). PubMed: 31572401
    • Pathak A  et al. Factor H binding proteins protect division septa on encapsulated Streptococcus pneumoniae against complement C3b deposition and amplification. Nat Commun 9:3398 (2018). PubMed: 30139996
    • Hu C  et al. Complement Inhibitor CRIg/FH Ameliorates Renal Ischemia Reperfusion Injury via Activation of PI3K/AKT Signaling. J Immunol 201:3717-3730 (2018). PubMed: 30429287
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-C3d antibody [7C10]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Diseased mouse kidney | paraffin sections)
    Antigen retrieval step
    Enzymatic - Buffer/Enzyme Used: Proteinase K
    Permeabilization
    No
    Specification
    Diseased mouse kidney | paraffin sections
    Blocking step
    Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Theodore Kaplan

    Verified customer

    Submitted Dec 21 2016

    Flow Cytometry abreview for Anti-C3d antibody [7C10]

    Good
    Abreviews
    Abreviews
    Application
    Flow Cytometry
    Sample
    Human Cell (ovary)
    Specification
    ovary
    Preparation
    Cell harvesting/tissue preparation method: trypsinization
    Sample buffer: PBS
    Fixation
    Paraformaldehyde
    Permeabilization
    No
    Gating Strategy
    live cells
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 11 2007

    Question

      Inquiry: What is the recommended dilution for WB with anti-C3d monoclonal (7C10)

    Read More

    Abcam community

    Verified customer

    Asked on Apr 26 2013

    Answer

    While the optimal concentration for use of this product will need to be determined experimentally in the lab, we recommend a starting dilution of 1ug/ml in Western blot.

    Read More

    Abcam Scientific Support

    Answered on Apr 26 2013

    Question

    I want to inquire whether it can detect C3b depositon about compelment activation, LPS was coated on the 96-well to innitiate complement activation.    

    Read More

    Abcam community

    Verified customer

    Asked on Jul 12 2011

    Answer

    Unfortunately I cannot not find much info on the epitope of this antibody. What I can say is that is will probable recognize C3d no matter how it is formed (could be LPS activation). As I do not know where the epitope is, I do not know if it will recognize C3dg, but I think not.

    Read More

    Abcam Scientific Support

    Answered on Jul 18 2011

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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