Anti-C5a / C5a des Arg antibody [2942] (ab11877)
![Immunocytochemistry/ Immunofluorescence - Anti-C5a / C5a des Arg antibody [2942] (ab11877)](https://www.abcam.com/ps/products/11/ab11877/Images/ab11877-196234-anti-c5a-c5a-des-arg-antibody-2942-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-C5a / C5a des Arg antibody [2942] (ab11877)")
Key features and details
- Mouse monoclonal [2942] to C5a / C5a des Arg
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-C5a / C5a des Arg antibody [2942]
See all C5a / C5a des Arg primary antibodies -
Description
Mouse monoclonal [2942] to C5a / C5a des Arg -
Host species
Mouse -
Specificity
This antibody reacts with a neo-epitope on C5a that is formed upon cleavage of C5 into C5a and C5b. It will detect C5a and C5a des-Arg (Not C5). -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Eye tissue of patient with age-related macular degeneration AMD IF/ICC: HeLa cell line.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
0.2 µm filtered -
Clonality
Monoclonal -
Clone number
2942 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab11877 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use a concentration of 10 µg/ml.
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| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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ICC/IF
Use a concentration of 10 µg/ml. |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
C5 is involved in the activation of the lytic pathway within the complement system, which is an important factor in innate immunity. -
Database links
- Entrez Gene: 727 Human
- Omim: 120900 Human
- SwissProt: P01031 Human
- SwissProt: Q14CJ0 Human
- SwissProt: Q27I61 Human
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Alternative names
- Anaphylatoxin C5a analog antibody
- C5 antibody
- C5a anaphylatoxin antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-C5a / C5a des Arg antibody [2942] (ab11877)
ICC/IF image of ab11877 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11877, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5a / C5a des Arg antibody [2942] (ab11877)](https://www.abcam.com/ps/products/11/ab11877/Images/ab11877-19128-anti-c5a-c5a-des-arg-antibody-2942-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5a / C5a des Arg antibody [2942] (ab11877)IHC image of ab11877 staining in normal human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11877, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab11877 has been referenced in 5 publications.
- Cho YT et al. Clinical, Histopathologic, and Immunohistochemical Features of Patients with IgG/IgA Pemphigus. Biomedicines 10:N/A (2022). PubMed: 35625932
- Corvillo F et al. Evidence of ongoing complement activation on adipose tissue from an 11-year-old girl with Barraquer-Simons syndrome. J Dermatol 47:1439-1444 (2020). PubMed: 32890433
- Neri T et al. Human pre-valvular endocardial cells derived from pluripotent stem cells recapitulate cardiac pathophysiological valvulogenesis. Nat Commun 10:1929 (2019). PubMed: 31028265
- Kajikawa T et al. Safety and Efficacy of the Complement Inhibitor AMY-101 in a Natural Model of Periodontitis in Non-human Primates. Mol Ther Methods Clin Dev 6:207-215 (2017). IHC-P ; Cynomolgus monkey . PubMed: 28879212
- Nozaki M et al. Drusen complement components C3a and C5a promote choroidal neovascularization. Proc Natl Acad Sci U S A 103:2328-33 (2006). PubMed: 16452172