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Anti-C5b-9 + C5b-8 antibody [aE11] (ab66768)

Reviews (1)Q&A (2)References (31)
Immunohistochemistry (Frozen sections) - Anti-C5b-9 + C5b-8 antibody [aE11] (ab66768)
  • Immunohistochemistry (Frozen sections) - Anti-C5b-9 + C5b-8 antibody [aE11] (ab66768)
  • Immunohistochemistry (Frozen sections) - Anti-C5b-9 + C5b-8 antibody [aE11] (ab66768)

Key features and details

  • Mouse monoclonal [aE11] to C5b-9 + C5b-8
  • Suitable for: ELISA, ICC/IF, Flow Cyt, IHC-Fr
  • Reacts with: Horse, Human, Pig, Monkey, Baboon
  • Isotype: IgG2a

Conjugates logo Related conjugates and formulations

Biotin

Overview

  • Product name

    Anti-C5b-9 + C5b-8 antibody [aE11]

  • Description

    Mouse monoclonal [aE11] to C5b-9 + C5b-8

  • Host species

    Mouse

  • Specificity

    The aE11 monoclonal was first reported to bind to a neoepitope exposed on polymerized C9 when incorporated into the human terminal complement complex (PubMed ID: 4035298). It was subsequently shown that this antibody also cross reacts with the C5b-8 complex and purified C8 alpha-gamma. It does not react with native C9 or C8 proteins (PubMed ID: 2424021).

    ab66768 binds both membrane-bound MAC (active) and fluid-phase SC5b-9 complexes (inactive) (Mollnes TE, Harboe M (1987).

  • Tested applications

    Suitable for: ELISA, ICC/IF, Flow Cyt, IHC-Frmore details
    Unsuitable for: WB

  • Species reactivity

    Reacts with: Horse, Human, Pig, Monkey, Baboon

  • Immunogen

    Full length protein corresponding to Human C5b-9.

  • Positive control

    • IHC-Fr: Human tonsil, monkey retina cord, and porcine liver tissues.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab66768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration. PubMed: 20688727
Flow Cyt
Use at an assay dependent concentration. PubMed: 23803904

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-Fr (1)
1/10 - 1/500.
Notes
ELISA
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration. PubMed: 20688727
Flow Cyt
Use at an assay dependent concentration. PubMed: 23803904

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-Fr
1/10 - 1/500.
Application notes
Is unsuitable for WB.

Target

Images

  • Immunohistochemistry (Frozen sections) - Anti-C5b-9 + C5b-8 antibody [aE11] (ab66768)
    Immunohistochemistry (Frozen sections) - Anti-C5b-9 + C5b-8 antibody [aE11] (ab66768)Image from Ekser B et al., PLoS One. 2012;7(1):e29720. Epub 2012 Jan 11. Fig 3.; doi:10.1371/journal.pone.0029720; January 11, 2012, PLoS ONE 7(1): e29720.

    ab66768 staining C5b-9 + C5b-8 (red) in porcine liver tissue by Immunohistochemistry (Frozen sections).

    Tissue was taken from allotransplants of wild-type or a1,3-galactosyltransferase gene-knockout (GTKO) pig liver, 2 hours after grafting or at necroscopy (nec). Tissue was fixed with paraformaldehyde and blocked with 20% donkey serum for 1 hour at room temperature, before being incubated with primary antibody (1/100 in diluent).

  • Immunohistochemistry (Frozen sections) - Anti-C5b-9 + C5b-8 antibody [aE11] (ab66768)
    Immunohistochemistry (Frozen sections) - Anti-C5b-9 + C5b-8 antibody [aE11] (ab66768)This image is courtesy of an anonymous Abreview

    ab66768 staining C5b-9 + C5b-8 in monkey retina cord tissue sections by Immunohistochemistry (IHC-Fr - frozen sections).

    Tissue was fixed with paraformaldehyde, permeabilized with 0.5% Triton-X and blocked with 4% serum for 30 minutes at 22°C. Samples were incubated with primary antibody (1/500) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-mouse IgG polyclonal (1/300) was used as the secondary antibody. Counterstained with DAPI. Magnification: 20X.

    See Abreview

  • Immunohistochemistry (Frozen sections) - Anti-C5b-9 + C5b-8 antibody [aE11] (ab66768)
    Immunohistochemistry (Frozen sections) - Anti-C5b-9 + C5b-8 antibody [aE11] (ab66768)

    Immunohistochemical analysis of frozen tonsil tissue section labeling C5b-9 + C5b-8 with ab66768 at a dilution of 1/10.

    Vascular endothelia and dendritic cells of germinal center have strong and intensive label.

     

     

References (31)

Publishing research using ab66768? Please let us know so that we can cite the reference in this datasheet.

ab66768 has been referenced in 31 publications.

  • Becker N  et al. The inflammatory pathology of dysferlinopathy is distinct from calpainopathy, Becker muscular dystrophy, and inflammatory myopathies. Acta Neuropathol Commun 10:17 (2022). PubMed: 35135626
  • Senage T  et al. The role of antibody responses against glycans in bioprosthetic heart valve calcification and deterioration. Nat Med 28:283-294 (2022). PubMed: 35177855
  • Cabrera AP  et al. Increased cell stiffness contributes to complement-mediated injury of choroidal endothelial cells in a monkey model of early age-related macular degeneration. J Pathol 257:314-326 (2022). PubMed: 35239183
  • Kolka CM  et al. C5b-9 Membrane Attack Complex Formation and Extracellular Vesicle Shedding in Barrett's Esophagus and Esophageal Adenocarcinoma. Front Immunol 13:842023 (2022). PubMed: 35345676
  • Pellegrini D  et al. Microthrombi as a Major Cause of Cardiac Injury in COVID-19: A Pathologic Study. Circulation 143:1031-1042 (2021). PubMed: 33480806
View all Publications for this product

Customer reviews and Q&As

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Submit a question

1-2 of 2 Q&A

Question

Could you provide datasheet of ab15981, ab136916, ab55811, ab66768?

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Answer

Thank you for contacting us.

The datasheet pdfs can be downloaded from Abcam website.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

I would like to test these antibodies in rabbit in IHC-P, which has not yet been tested.

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Answer

I am very pleased to hear you would like to accept our offer and test ab6678 in rabbit, ab8878 in IHC-P, and ab11862 in rabbit. These codes will give you 1 free primary antibody per code before the expiration date. To redeem this offer, please submit an Abreview for each antibody and include the code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The codes will be active once the Abreviews have been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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