Recombinant Anti-C8G antibody [EPR11274] - C-terminal (ab181182)
![Western blot - Anti-C8G antibody [EPR11274] - C-terminal (ab181182)](https://www.abcam.com/ps/products/181/ab181182/Images/ab181182-214706-anti-c8g-antibody-epr11274-c-terminal-western-blot.jpg "Western blot - Anti-C8G antibody [EPR11274] - C-terminal (ab181182)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11274] to C8G - C-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-C8G antibody [EPR11274] - C-terminal
See all C8G primary antibodies -
Description
Rabbit monoclonal [EPR11274] to C8G - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human colon cancer lysate and plasma. SW480 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11274 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181182 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/2000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
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| ICC/IF |
1/50 - 1/100.
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| Notes |
|---|
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WB
1/1000 - 1/2000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). |
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ICC/IF
1/50 - 1/100. |
Target
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Function
C8 is a constituent of the membrane attack complex. C8 binds to the C5B-7 complex, forming the C5B-8 complex. C5-B8 binds C9 and acts as a catalyst in the polymerization of C9. The gamma subunit seems to be able to bind retinol. -
Sequence similarities
Belongs to the calycin superfamily. Lipocalin family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 733 Human
- Entrez Gene: 69379 Mouse
- Entrez Gene: 296545 Rat
- Omim: 120930 Human
- SwissProt: P07360 Human
- SwissProt: Q8VCG4 Mouse
- Unigene: 1285 Human
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Alternative names
- C8C antibody
- C8G antibody
- CO8G_HUMAN antibody
see all
Images
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Western blot - Anti-C8G antibody [EPR11274] - C-terminal (ab181182)All lanes : Anti-C8G antibody [EPR11274] - C-terminal (ab181182) at 1/1000 dilution
Lane 1 : Human colon cancer lysate
Lane 2 : Human plasma
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 22 kDa
Observed band size: 22 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-C8G antibody [EPR11274] - C-terminal (ab181182)](https://www.abcam.com/ps/products/181/ab181182/Images/ab181182-214707-anti-c8g-antibody-epr11274-c-terminal-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-C8G antibody [EPR11274] - C-terminal (ab181182)Immunofluorescence analysis of Human SW480 cells, staining C8G (red) with ab181182 at 1/100 dilution. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG was used as a secondary antibody at 1/200 dilution. Nuclei were counterstained with DAPI (blue).
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![Anti-C8G antibody [EPR11274] - C-terminal (ab181182)](https://www.abcam.com/ps/products/181/ab181182/Images/ab181182-3-benefits-of-recombinant-antibodies.png)
Anti-C8G antibody [EPR11274] - C-terminal (ab181182)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab181182 has not yet been referenced specifically in any publications.