Name: Anti-CACNA1C antibody [S57]
SKU: ab84814
Rating: 5 (6 reviews)

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Western blot - Anti-CACNA1C antibody [S57] (ab84814)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Mouse monoclonal [S57] to CACNA1C
Suitable for: ICC/IF, IHC-P, WB, Flow Cyt
Reacts with: Mouse, Human, Recombinant fragment

Product name

Anti-CACNA1C antibody [S57]
See all CACNA1C primary antibodies
Description

Mouse monoclonal [S57] to CACNA1C
Host species

Mouse
Tested applications

Suitable for: ICC/IF, IHC-P, WB, Flow Cytmore details
Species reactivity

Reacts with: Mouse, Human, Recombinant fragment
Immunogen

Fusion protein corresponding to Rabbit CACNA1C aa 1500-1750 (C terminal).
Database link: P15381
Positive control
- WB: Cell lysates prepared from DHPR alpha 1 transfected CHO cells. IHC-P: Human hippocampus tissue; Mouse brain tissue. ICC/IF: SK-N-BE cells. Flow Cyt: SH-SY5Y cells.
General notes

The clone number has been updated from S57-46 to L57/46, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer

Preservative: 0.1% Sodium azide
Constituents: 50% Glycerol, PBS
Concentration information loading...
Purity

Protein G purified
Clonality

Monoclonal
Clone number

S57
Isotype

IgG1
Research areas
- Neuroscience
- Processes

Compatible Secondaries
Positive Controls
- Rat brain normal tissue lysate - membrane extract (ab29473)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab84814 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
ICC/IF		1/100.
IHC-P		Use a concentration of 0.1 - 10 µg/ml. Use at 0.1-1.0µg/mL (perox), 1.0-10µg/mL (IF).
WB		Use a concentration of 1 µg/ml. Detects a band of approximately 240 kDa. If results are poor, use lysate without boiling, heat at 37°C for 15 minutes.
Flow Cyt		Use 1µg for 10⁶ cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Notes
ICC/IF 1/100.
IHC-P Use a concentration of 0.1 - 10 µg/ml. Use at 0.1-1.0µg/mL (perox), 1.0-10µg/mL (IF).
WB Use a concentration of 1 µg/ml. Detects a band of approximately 240 kDa. If results are poor, use lysate without boiling, heat at 37°C for 15 minutes.
Flow Cyt Use 1µg for 10⁶ cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Function

Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1C gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin-GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing the alpha-1C subunit play an important role in excitation-contraction coupling in the heart. The various isoforms display marked differences in the sensitivity to DHP compounds. Binding of calmodulin or CABP1 at the same regulatory sites results in an opposit effects on the channel function.
Tissue specificity

Expressed in brain, heart, jejunum, ovary, pancreatic beta-cells and vascular smooth muscle. Overall expression is reduced in atherosclerotic vascular smooth muscle.
Involvement in disease

Timothy syndrome
Brugada syndrome 3
Sequence similarities

Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1C subfamily.
Domain

Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.
Binding of intracellular calcium through the EF-hand motif inhibits the opening of the channel.
Post-translational
modifications

Phosphorylation by PKA activates the channel.
Cellular localization

Membrane. Cell membrane. The interaction between RRAD and CACNB2 regulates its trafficking to the cell membrane.
Target information above from: UniProt accession Q13936 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 775 Human
- Entrez Gene: 12288 Mouse
- Omim: 114205 Human
- SwissProt: Q13936 Human
- SwissProt: Q01815 Mouse
- Unigene: 118262 Human
- Unigene: 690010 Human
- Unigene: 41628 Mouse
- Unigene: 436656 Mouse
see all
Alternative names
- alpha-1 polypeptide antibody
- cardiac muscle antibody
- isoform 1 antibody
- L type antibody
- CAC1C_HUMAN antibody
- CACH 2 antibody
- CACH2 antibody
- CACN 2 antibody
- CACN2 antibody
- CACNA1C antibody
- CACNL1A1 antibody
- Calcium channel antibody
- Calcium channel cardic dihydropyridine sensitive alpha 1 subunit antibody
- Calcium channel L type alpha 1 polypeptide isoform 1 cardiac muscle antibody
- Calcium channel voltage dependent L type alpha 1C subunit antibody
- CaV1.2 antibody
- CCHL1A1 antibody
- DHPR alpha 1 antibody
- DHPR alpha 1 subunit antibody
- LQT8 antibody
- TS antibody
- Voltage dependent L type calcium channel alpha 1C subunit antibody
- Voltage dependent L type calcium channel subunit alpha 1C antibody
- Voltage gated calcium channel alpha subunit Cav1.2 antibody
- Voltage gated calcium channel subunit alpha Cav1.2 antibody
- Voltage gated L type calcium channel Cav1.2 alpha 1 subunit, splice variant 10* antibody
- Voltage-dependent L-type calcium channel subunit alpha-1C antibody
- Voltage-gated calcium channel subunit alpha Cav1.2 antibody
see all

Western blot - Anti-CACNA1C antibody [S57] (ab84814)

All lanes : Anti-CACNA1C antibody [S57] (ab84814) at 1 µg/ml

Lane 1 : Molecular weight marker
Lane 2 : Cell lysates prepared from DHPR alpha 1 transfected CHO cells
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1C antibody [S57] (ab84814)

ab84814 staining CACNA1C in human hippocampus tissue section by Immunohistochemistry (Bouin's fixative fixed paraffin embedded tissue sections). Tissue underwent heat mediate fixation in microwave and in citrate buffer. The primary antibody was used at 1/100 dilution. A Fluorophore conjugated goat anti-mouse was used as secondary at 1/50 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CACNA1C antibody [S57] (ab84814)

ab84814 staining CACNA1C in mouse brain tissue section by immunohistochemistry (Bouin's fixed paraffin embedded tissue section. Tissue underwent heat mediated fixation in microwave and in citrate buffer. The primary antibody was used at 1/100 dilution. A HRP conjugated secondary was used at 10 dilution.
Flow Cytometry - Anti-CACNA1C antibody [S57] (ab84814)

Overlay histogram showing SH-SY5Y cells stained with ab84814 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab84814, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.
Immunocytochemistry/ Immunofluorescence - Anti-CACNA1C antibody [S57] (ab84814)

Formaldehyde-fixed SK-N-BE cells stained for CACNA1C (green) using ab84814 at 1/100 dilution in ICC/IF.

Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1/200 dilution for 60 minutes at room temperature. Counterstain: Phalloidin Texas Red F-Actin stain (A); DAPI (B, blue) nuclear stain at 1/1000, 1/5000 for 60 minutes at room temperature, 5 minutes at room temperature.

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab84814? Please let us know so that we can cite the reference in this datasheet.

ab84814 has been referenced in 23 publications.

Adili A et al. Atrial Fibrillation Underlies Cardiomyocyte Senescence and Contributes to Deleterious Atrial Remodeling during Disease Progression. Aging Dis 13:298-312 (2022). PubMed: 35111375
Jiang X et al. MCC950 ameliorates ventricular arrhythmia vulnerability induced by heart failure. Bioengineered 13:8593-8604 (2022). PubMed: 35287557
Huang Y et al. Nisoldipine Inhibits Influenza A Virus Infection by Interfering with Virus Internalization Process. Viruses 14:N/A (2022). PubMed: 36560742
Jiang T et al. IL-6/STAT3 Signaling Promotes Cardiac Dysfunction by Upregulating FUNDC1-Dependent Mitochondria-Associated Endoplasmic Reticulum Membranes Formation in Sepsis Mice. Front Cardiovasc Med 8:790612 (2021). PubMed: 35118141
Song Q et al. Astragaloside IV protects against retinal iron overload toxicity through iron regulation and the inhibition of MAPKs and NF-?B activation. Toxicol Appl Pharmacol 410:115361 (2021). PubMed: 33285147

View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A

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1-10 of 15 Abreviews or Q&A

Application

Immunohistochemistry (Frozen sections)

Sample

Horse Tissue sections (Skeletal muscles)

Specification

Skeletal muscles

Fixative

Acetone

Permeabilization

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 17 2012

Application

Immunohistochemistry (Frozen sections)

Sample

Dog Tissue sections (Skeletal muscles)

Specification

Skeletal muscles

Fixative

Acetone/methanol 1:1

Permeabilization

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 03 2012

Application

Immunohistochemistry (Frozen sections)

Sample

Human Tissue sections (atrium)

Specification

atrium

Fixative

Paraformaldehyde

Permeabilization

Yes - triton-X 100

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 17 2012

Application

Immunohistochemistry (Frozen sections)

Sample

Mouse Tissue sections (heart)

Specification

heart

Fixative

Paraformaldehyde

Permeabilization

Yes - triton-X 100

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 6% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 17 2012

Question

I would like to test ab84814 for use on dog samples.

Abcam community

Verified customer

Asked on Jun 30 2012

Answer

DISCOUNT CODE: ***
Expiration date: October 29th, 2012

I am very pleased to hear you would like to accept our offer and test ab84814 in dog. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview fordog and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Abcam Scientific Support

Answered on Jun 30 2012

Question

ReviewID: xxx AbID: 84814, Anti-DHPR alpha 1 antibody [S57-46] Rating: Inconclusive Image: Anti-DHPR alpha 1 antibody [S57-46] for Immunohistochemistry (Frozen sections) (Human) Sample: Species: Human Type: Tissue sections Specification: atrium Application: Application: Fixative: Paraformaldehyde Permeabilization: Yes Blocking step: BSA as blocking agent for 1 hour at 25°C Other detail: Dilution: 1/50 Incubation time: 12 hours at 4°C Diluent: PBS+BSA+serum Secondary Antibody: Name: Non-Abcam Antibody was used: goat anti mouse IgG2b488 Conjugation: Alexa Fluor® 488 Dilution: 1/500 Additional Data: Additional Notes: only see non-specifc binding

Abcam community

Verified customer

Asked on Apr 27 2012

Answer

According to our records, ab84814 was proving difficult to use in IHC-Fr and we were in contact in order to help resolve the issue.

Looking at our correspondence, it appears that we are awaiting more details in order to help us better understand the difficulties experienced.

Based on the information in your review, I feel that altering the following step in your protocol may help improve your results:

1) Trying another fixative might help to solve the problem as the PFA fixation might crosslink antibody binding sites.
2) You could also try omitting the permeabilization step if your sections are thin enough (10 um or less)
3) Doing a no primary control could help to determine if the secondary antibody contributes to the non-specific staining.
4) What is you order or PO number?

If you decide to follow these suggestions, please let me know if they are helpful. Additionally, this antibody is covered by our Abpromise, so if the suggestions do not help and the antibody was purchased within the past 6 months, I would be happy to discuss a refund or replacement with you to resolve this issue.

If the requested information has already been sent, it appears that it did not reach our Scientific Support team and we apologize for this inconvenience. In this case we would like to ask for the information again so that we can reach a resolution.

If the issue has already been settled, please let us know so that we can be assured that the problem has been solved to your satisfaction and update our records.

We wish you the best of luck with your research and look forward to a reply.

Abcam Scientific Support

Answered on Apr 27 2012

Question

Abcam community

Verified customer

Asked on Apr 18 2012

Answer

Thank you for submitting an Abreview of ab84814. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody in a tested species and application, we would like to follow up on this to see if we can possibly improve the results you are seeing with this antibody.

Based on the information in your review, I feel that altering the following step in your protocol may help improve your results:

1) Trying another fixative might help to solve the problem as the PFA fixation might crosslink antibody binding sites.
2) You could also try omitting thepermeabilization step if your sections are thin enough (10 um or less).
3) Doing ano primary control could help to determine if the secondary antibody contributes to the non-specific staining.
4) What is you order or PO number?

If you decide to follow these suggestions, please let me know if they are helpful. Additionally, this antibody is covered by our Abpromise, so if the suggestions do not help and the antibody was purchased within the past 6 months, I would be happy to discuss a refund or replacement with you to resolve this issue.

I look forward to your reply.

Abcam Scientific Support

Answered on Apr 18 2012

Question

Thank you for your former advice and replacement antibody for DHPR alpha 1 (aka CaV 1.2 alpha 1).

Now we have tried Ab 81980 with our human heart samples, but unfortunately it does not seem to work either, as it gives some aspecific band with a smaller MW than expected and no band occurs at the expected MW range.

I attach a ppt slide with the result we have got.

In the meanwhile, we have tried another antibody and that one works fine, the band coming at the right MW and control peptide blocking showed that it was a specific band. We are going to use that antibody for our further studies.

I would like to ask if we can still get a refund or credit which we can spend purhasing some other (not CaV 1.2) antibody?

Abcam community

Verified customer

Asked on Feb 21 2012

Answer

Thank you for your reply and for completing of customer survey follow up email.
I am sorry that the replacement antibody that we sent you, ab81980 did not prove to be successful for you.
Therefore I have processed your request to receive a refund for the original antibody, ab81814.
Please let me know if there is anything else I can help you with.

Abcam Scientific Support

Answered on Feb 21 2012

Question

Thank you for your advice, after looking at the data sheets one more time, I also think that ab81980 has the better chances, and I was impressed by the blot presented on the data sheet.

So we would like to try ab81980, would you be so kind to sent that one?

Thank you,

Abcam community

Verified customer

Asked on Jan 18 2012

Answer

Thank you for contacting Abcam.
I have processed your request to receive ab81980 as a replacement for ab84814 and your new order number is ********.
I hope that this new antibody is successful and if there is anything else I can help you with, please let me know.

Abcam Scientific Support

Answered on Jan 18 2012

Question

Thank you for your help. The ordering information is correct, that is us.

I will have one more look at those antibody fact sheets today and decide which one to try - do you happen to know if any of them has good references for western blot, preferably on human samples?

Abcam community

Verified customer

Asked on Jan 18 2012

Answer

Thank you for reply.
Out of either ab81980 or ab8109, I would recommend ab81980, as we have a western blot image for that one on our website. We do not have any more data for either antibody, although both are guaranteed to work in western blot on human samples.
I look forward to your reply and helping you resolve this issue.

Abcam Scientific Support

Answered on Jan 18 2012

1-10 of 15 Abreviews or Q&A

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Key features and details

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Compatible Secondaries

Positive Controls

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Involvement in disease

Sequence similarities

Domain

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (23)

Customer reviews and Q&As

Immunohistochemistry (Frozen sections) abreview for Anti-DHPR alpha 1 antibody [S57-46]

Immunohistochemistry (Frozen sections) abreview for Anti-DHPR alpha 1 antibody [S57-46]

Immunohistochemistry (Frozen sections) abreview for Anti-DHPR alpha 1 antibody [S57-46]

Immunohistochemistry (Frozen sections) abreview for Anti-DHPR alpha 1 antibody [S57-46]

Post-translational
modifications