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RecombinantRabMAb

Recombinant Anti-Cadherin 16 antibody [EPR13090] (ab183745)

Reviews (1) Submit a question
Western blot - Anti-Cadherin 16 antibody [EPR13090] (ab183745)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [EPR13090] (ab183745)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [EPR13090] (ab183745)
  • Immunoprecipitation - Anti-Cadherin 16 antibody [EPR13090] (ab183745)
  • Anti-Cadherin 16 antibody [EPR13090] (ab183745)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13090] to Cadherin 16
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Cadherin 16 antibody [EPR13090]
    See all Cadherin 16 primary antibodies

  • Description

    Rabbit monoclonal [EPR13090] to Cadherin 16

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal kidney lysate; Human chromophobe renal cell carcinoma and mouse kidney tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR13090

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab183745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 130 kDa (predicted molecular weight: 90 kDa).
IHC-P
1/4000 - 1/8000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/40 - 1/60.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 130 kDa (predicted molecular weight: 90 kDa).
IHC-P
1/4000 - 1/8000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/40 - 1/60.

Target

  • Function

    Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.

  • Tissue specificity

    Kidney specific.

  • Sequence similarities

    Contains 6 cadherin domains.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • CAD16_HUMAN antibody
      • Cadherin 16 antibody
      • Cadherin 16 KSP cadherin antibody
      • Cadherin kidney antibody
      • Cadherin-16 antibody
      • CDH 16 antibody
      • CDH16 antibody
      • Kidney cadherin antibody
      • Kidney specific cadherin antibody
      • Kidney-specific cadherin antibody
      • Ksp cadherin antibody
      • Ksp-cadherin antibody
      • PRO1340 antibody
      • UNQ695 antibody
      see all

    Images

    • Western blot - Anti-Cadherin 16 antibody [EPR13090] (ab183745)
      Western blot - Anti-Cadherin 16 antibody [EPR13090] (ab183745)
      Anti-Cadherin 16 antibody [EPR13090] (ab183745) at 1/10000 dilution + Human fetal kidney lysate at 10 µg

      Secondary
      Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution

      Predicted band size: 90 kDa
      Observed band size: 130 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [EPR13090] (ab183745)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [EPR13090] (ab183745)

      Immunohistochemical analysis of paraffin-embedded Human chromophobe renal cell carcinoma tissue labeling Cadherin 16 with ab183745 at 1/4000 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [EPR13090] (ab183745)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [EPR13090] (ab183745)

      Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Cadherin 16 with ab183745 at 1/4000 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with hematoxylin.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-Cadherin 16 antibody [EPR13090] (ab183745)
      Immunoprecipitation - Anti-Cadherin 16 antibody [EPR13090] (ab183745)

      Western blot analysis of Cadherin 16 in Human fetal kidney lysate immunoprecipitated using ab183745 at 1/50 dilution.

      Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

    • Anti-Cadherin 16 antibody [EPR13090] (ab183745)
      Anti-Cadherin 16 antibody [EPR13090] (ab183745)

    References (0)

    Publishing research using ab183745? Please let us know so that we can cite the reference in this datasheet.

    ab183745 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-Cadherin 16 antibody [EPR13090]

     Inconclusive
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Cow Tissue lysate - whole (kidney)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    10 µg
    Specification
    kidney
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 07 2016

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com