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RecombinantRabMAb

Recombinant Anti-Calcitonin antibody [SP17] (ab16697)

Submit a review Submit a question References (1)
Immunocytochemistry/ Immunofluorescence - Anti-Calcitonin antibody [SP17] (ab16697)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin antibody [SP17] (ab16697)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin antibody [SP17] (ab16697)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin antibody [SP17] (ab16697)
  • Anti-Calcitonin antibody [SP17] (ab16697)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP17] to Calcitonin
  • Suitable for: IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Calcitonin antibody [SP17]
    See all Calcitonin primary antibodies

  • Description

    Rabbit monoclonal [SP17] to Calcitonin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • Positive control

    • IHC-P: Human thyroid medullary carcinoma, pituitary and lung adenocarcinoma tissue. ICC/IF: TT cells

  • General notes

    This product has switched from a hybridoma to recombinant production format on 22nd June 2020.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab16697 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/100.
ICC/IF
1/50.
Notes
IHC-P
1/100.
ICC/IF
1/50.

Target

  • Relevance

    Calcitonin is a 32 amino acid peptide hormone synthesized by the parafollicular cells of the thyroid. It causes a rapid, but short lived, reduction in serum calcium and phosphate by promoting the incorporation of those ions in the bones. This effect is opposite to that of parathyroid hormone. Staining for calcitonin may be used for the identification of a spectrum of C cell proliferative abnormalities ranging from C cell hyperplasia to invasive tumors. Staining for calcitonin in medullary carcinoma of the thyroid produces a fine granular pattern in the cytoplasm. Amyloid deposits within the tumor may also exhibit varying degrees of calcitonin activity.

  • Cellular localization

    Cytoplasmic and Secreted

  • Database links

    • Alternative names

      • Alpha CGRP antibody
      • alpha type CGRP antibody
      • CALC 1 antibody
      • CALC A antibody
      • CALC1 antibody
      • calcitonin 1 antibody
      • calcitonin gene related peptide 1 antibody
      • calcitonin gene related peptide I antibody
      • calcitonin related polypeptide alpha antibody
      • CGRP 1 antibody
      • CGRP antibody
      • CGRP I antibody
      • CGRP1 antibody
      • CT antibody
      • katacalcin antibody
      • KC antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Calcitonin antibody [SP17] (ab16697)
      Immunocytochemistry/ Immunofluorescence - Anti-Calcitonin antibody [SP17] (ab16697)

      Immunocytochemistry/ Immunofluorescence analysis of TT (Human thyroid carcinoma epithelial cell) cells labeling Calcitonin with purified ab16697 at 1/50 (2.4 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

      This image was generated using the hybridoma version of the product.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin antibody [SP17] (ab16697)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin antibody [SP17] (ab16697)

      Formalin-fixed, paraffin-embedded human thyroid medullary carcinoma tissue stained for Calcitonin using ab16697 at 1/100 dilution in immunohistochemical analysis.

      This image was generated using the hybridoma version of the product.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin antibody [SP17] (ab16697)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin antibody [SP17] (ab16697)

      Formalin-fixed, paraffin-embedded human pituitary tissue stained for Calcitonin using ab16697 at 1/100 dilution in immunohistochemical analysis.

      This image was generated using the hybridoma version of the product.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin antibody [SP17] (ab16697)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcitonin antibody [SP17] (ab16697)

      Formalin-fixed, paraffin-embedded human lung adenocarcinoma tissue stained for Calcitonin using ab16697 at 1/100 dilution in immunohistochemical analysis.

      This image was generated using the hybridoma version of the product.

    • Anti-Calcitonin antibody [SP17] (ab16697)
      Anti-Calcitonin antibody [SP17] (ab16697)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab16697? Please let us know so that we can cite the reference in this datasheet.

    ab16697 has been referenced in 1 publication.

    • Takeshima K  et al. False-positive staining of thyroglobulin distinguished from mixed medullary and follicular thyroid carcinoma by duplex in situ hybridization. Endocr J 67:1007-1017 (2020). PubMed: 32522910

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com