Recombinant Anti-Caldesmon/CDM antibody [SP226] (ab183339)
![Western blot - Anti-Caldesmon/CDM antibody [SP226] (ab183339)](https://www.abcam.com/ps/products/183/ab183339/Images/ab183339-391319-anti-caldesmoncdm-antibody-sp226-ab183339-western-blot-human-knockout-lysate.jpg "Western blot - Anti-Caldesmon/CDM antibody [SP226] (ab183339)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP226] to Caldesmon/CDM
- Suitable for: Flow Cyt (Intra), IHC-P, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Caldesmon/CDM antibody [SP226]
See all Caldesmon/CDM primary antibodies -
Description
Rabbit monoclonal [SP226] to Caldesmon/CDM -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa and NIH/3T3 cell lysates; smooth muscle cell lysate. IHC-P: Human colon tissue; Mouse colon tissue; Rat colon Tissue Flow Cyt (intra): HeLa
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General notes
Chicken: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
SP226 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183339 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/230 - 1/400.
Incubate for 30 minutes at 4°C. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| IHC-P |
1/100.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol by boiling tissue section in EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 10 minutes at room temperature. |
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| WB |
1/400. Predicted molecular weight: 93 kDa.
Incubate for 1 hour at room temperature. |
| Notes |
|---|
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Flow Cyt (Intra)
1/230 - 1/400. Incubate for 30 minutes at 4°C. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol by boiling tissue section in EDTA buffer, pH 8.0 for 10 min followed by cooling at room temperature for 20 min. Primary Antibody Incubation: Incubate for 10 minutes at room temperature. |
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WB
1/400. Predicted molecular weight: 93 kDa. Incubate for 1 hour at room temperature. |
Target
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Function
Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping. -
Tissue specificity
High-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular-weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart. -
Sequence similarities
Belongs to the caldesmon family. -
Domain
The N-terminal part seems to be a myosin/calmodulin-binding domain, and the C-terminal a tropomyosin/actin/calmodulin-binding domain. These two domains are separated by a central helical region in the smooth-muscle form. -
Post-translational
modificationsIn non-muscle cells, phosphorylation by CDK1 during mitosis causes caldesmon to dissociate from microfilaments. Phosphorylation reduces caldesmon binding to actin, myosin, and calmodulin as well as its inhibition of actomyosin ATPase activity. Phosphorylation also occurs in both quiescent and dividing smooth muscle cells with similar effects on the interaction with actin and calmodulin and on microfilaments reorganization. -
Cellular localization
Cytoplasm > cytoskeleton. Cytoplasm > myofibril. On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle). - Information by UniProt
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Database links
- Entrez Gene: 800 Human
- Entrez Gene: 109624 Mouse
- Entrez Gene: 25687 Rat
- Omim: 114213 Human
- SwissProt: Q05682 Human
- SwissProt: Q62736 Rat
- Unigene: 490203 Human
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Alternative names
- CAD antibody
- CALD 1 antibody
- CALD1 antibody
see all
Images
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Western blot - Anti-Caldesmon/CDM antibody [SP226] (ab183339)All lanes : Anti-Caldesmon/CDM antibody [SP226] (ab183339) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : CALD1 knockout HeLa cell lysate
Lane 3 : NIH/3T3 cell lysate
Lane 4 : HEK-293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 93 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab183339 observed at 75 kDa. Red - loading control ab8245 observed at 36 kDa.
ab183339 Anti-Caldesmon/CDM antibody [SP226] was shown to specifically react with Caldesmon/CDM in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265026 (knockout cell lysate ab257375) was used. Wild-type and Caldesmon/CDM knockout samples were subjected to SDS-PAGE. ab183339 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caldesmon/CDM antibody [SP226] (ab183339)](https://www.abcam.com/ps/products/183/ab183339/Images/ab183339-7-AntiCaldesmonCDM-antibody-SP226-IHC-rat-colon.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caldesmon/CDM antibody [SP226] (ab183339)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat colon tissue sections labeling Caldesmon/CDM with ab183339 at 1/100 dilution (23.11 µg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on smooth muscle in rat colon, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab183339 for 10 mins at room temperature. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caldesmon/CDM antibody [SP226] (ab183339)](https://www.abcam.com/ps/products/183/ab183339/Images/ab183339-6-AntiCaldesmonCDM-antibody-SP226-IHC-mouse-colon.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caldesmon/CDM antibody [SP226] (ab183339)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse colon tissue sections labeling Caldesmon/CDM with ab183339 at 1/100 dilution (23.11 µg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on smooth muscle in mouse colon, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab183339 for 10 mins at room temperature. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caldesmon/CDM antibody [SP226] (ab183339)](https://www.abcam.com/ps/products/183/ab183339/Images/ab183339-5-AntiCaldesmonCDM-antibody-SP226-IHC-human-colon.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caldesmon/CDM antibody [SP226] (ab183339)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling Caldesmon/CDM with ab183339 at 1/100 dilution (23.11 µg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on smooth muscle in human colon, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab183339 for 10 mins at room temperature. -
![Western blot - Anti-Caldesmon/CDM antibody [SP226] (ab183339)](https://www.abcam.com/ps/products/183/ab183339/Images/ab183339-217098-anti-caldesmon-antibody-sp226-western-blot.jpg)
Western blot - Anti-Caldesmon/CDM antibody [SP226] (ab183339)Anti-Caldesmon/CDM antibody [SP226] (ab183339) at 1/400 dilution + Smooth muscle cell lysate
Predicted band size: 93 kDa -
![Flow Cytometry (Intracellular) - Anti-Caldesmon/CDM antibody [SP226] (ab183339)](https://www.abcam.com/ps/products/183/ab183339/Images/ab183339-330578-anti-caldesmon-antibody-sp226-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Caldesmon/CDM antibody [SP226] (ab183339)Intracellular Flow Cytometry analysis of Hela (human cervix adenocarcinoma) labeling Caldesmon/CDM with purified ab183339 at 1/230 dilution (10.05µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlableled control - Unlabelled cells (blue).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caldesmon/CDM antibody [SP226] (ab183339)](https://www.abcam.com/ps/products/183/ab183339/Images/ab183339-217099-anti-caldesmon-antibody-sp226-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caldesmon/CDM antibody [SP226] (ab183339)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon tissue labeling Caldesmon/CDM with ab183339 at 1/100 dilution.
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![Flow Cytometry (Intracellular) - Anti-Caldesmon/CDM antibody [SP226] (ab183339)](https://www.abcam.com/ps/products/183/ab183339/Images/ab183339-217021-anti-caldesmon-antibody-sp226-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Caldesmon/CDM antibody [SP226] (ab183339)Intracellular flow cytometric analysis of HeLa cells labeling Caldesmon/CDM with ab183339 at 1/400 dilution (green) compared to a negative control Rabbit IgG (blue).
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![Anti-Caldesmon/CDM antibody [SP226] (ab183339)](https://www.abcam.com/ps/products/183/ab183339/Images/ab183339-10-benefits-of-recombinant-antibodies.png)
Anti-Caldesmon/CDM antibody [SP226] (ab183339)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab183339 has been referenced in 1 publication.
- Yu B et al. Prediction of lymph node metastasis in oral squamous cell carcinoma based on protein profile. Expert Rev Proteomics 16:363-373 (2019). PubMed: 30779878