Anti-Calreticulin antibody - ER Marker (ab2907)
Key features and details
- Rabbit polyclonal to Calreticulin - ER Marker
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
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Overview
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Product name
Anti-Calreticulin antibody - ER Marker
See all Calreticulin primary antibodies -
Description
Rabbit polyclonal to Calreticulin - ER Marker -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein corresponding to Human Calreticulin.
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Positive control
- WB: HL-60, LNCaP, HeLa and MCF-7 cell lysates; Mouse and rat liver tissue lysates; Mouse skeletal muscle whole cell lysate. ICC/IF: A431, HeLa, U2OS, HepG2 and HMVEC cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2907 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (8) |
Use at an assay dependent concentration.
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| WB | (4) |
1/1000.
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| Notes |
|---|
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ICC/IF
Use at an assay dependent concentration. |
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WB
1/1000. |
Target
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Function
Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. -
Sequence similarities
Belongs to the calreticulin family. -
Domain
Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity.
The interaction with glycans occurs through a binding site in the globular lectin domain.
The zinc binding sites are localized to the N-domain.
Associates with PDIA3 through the tip of the extended arm formed by the P-domain. -
Cellular localization
Endoplasmic reticulum lumen. Cytoplasm > cytosol. Secreted > extracellular space > extracellular matrix. Cell surface. Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes. - Information by UniProt
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Database links
- Entrez Gene: 811 Human
- Entrez Gene: 12317 Mouse
- Entrez Gene: 64202 Rat
- Omim: 109091 Human
- SwissProt: P27797 Human
- SwissProt: P14211 Mouse
- SwissProt: P18418 Rat
- Unigene: 515162 Human
see all -
Alternative names
- Autoantigen RO antibody
- CALR antibody
- CALR protein antibody
see all
Images
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Western blot - Anti-Calreticulin antibody - ER Marker (ab2907)All lanes : Anti-Calreticulin antibody - ER Marker (ab2907) at 1/1000 dilution
Lane 1 : HL-60 cell lysate
Lane 2 : LNCaP cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : MCF-7 cell lysate
Lane 5 : Mouse liver tissue lysate
Lane 6 : Rat liver tissue lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution
Observed band size: 55 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)Image from Yount JS et al, J Biol Chem. 2012 Jun 1;287(23):19631-41. Epub 2012 Apr 17, Fig 3. DOI 10.1074/jbc.M112.362095 June 1, 2012 The Journal of Biological Chemistry, 287, 19631-19641.ab2907 used at a 1/1000 dilution staining Calreticulin in HeLa cells by Immunocytochemistry/ Immunofluorescence.
HeLa cells were transfected overnight with empty vector or plasmids encoding the indicated IFITM3 constructs. Immunofluorescence with a-HA antibodies allowed IFITM3 visualization, and a-calreticulin staining allowed visualization of the ER. TOPRO-3 was used to visualize nuclei. Scale bars indicate 10 µm. Ub? indicates mutation of Lys-24, Lys-83, Lys-88, and Lys-104 to alanine. -
Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)Immunocytochemistry/Immunofluorescence analysis of Calreticulin (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were incubated with ab2907 (1:75) for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (1:250) for 30 minutes at room temperature. Actin was stained with DyLight 650 Phalloidin (1:120) and nuclei (blue) were stained with Hoechst (1ug/ml) for 30 minutes. Images were taken at 20X magnification.
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Western blot - Anti-Calreticulin antibody - ER Marker (ab2907)This image is courtesy of an anonymous AbreviewAll lanes : Anti-Calreticulin antibody - ER Marker (ab2907) at 1/1000 dilution
All lanes : Whole cell lysate prepared from mouse skeletal muscle
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated mouse polyclonal to rabbit Ig at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 3 seconds
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Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)Immunocytochemistry/Immunofluorescence analysis of Calreticulin (red) in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were incubated with ab2907 (1:75) for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 633 goat anti-rabbit IgG secondary antibody (1:250) for 30 minutes at room temperature. Actin was stained with DyLight 488 Phalloidin (1:300) and nuclei (blue) were stained with Hoechst (1ug/ml) for 30 minutes. Images were taken at 20X magnification.
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Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)Immunocytochemsitry/Immunofluorescence analysis of Calreticulin (green) U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS 0.1% triton-X for 30 minutes at room temperature. Cells were incubated with ab2907 (1:50) for at least 1 hour at room temperature. Cells were washed with PBS and incubated with DyLight 488 goat-anti-rabbit IgG secondary antibody (1:250) for 30 minutes at room temperature. Actin filaments (red) were stained with DyLight 554-Phalloidin (1:300) in PBS and incubated for 30 minutes. Nuclei (blue) were stained with Hoechst 33342 dye (1µg/mL). Images were taken at 20X magnification.
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Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)Immunocytochemistry/Immunofluorescence analysis of Calreticulin (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were incubated with ab2907 (1:75) for at least 1 hour at room temperature, washed with PBS, and incubated with Dylight 488 goat anti-rabbit IgG secondary antibody (1:250) for 30 minutes at room temperature. Actin was stained with Dylight 350 Phalloidin (1:120) and nuclei (red) were stained with DRAQ5 (1ug/ml) for 30 minutes. Images were taken at 20X magnification.
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Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)Immunocytochemistry/Immunofluorescence analysis of Calreticulin (green) in A431 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were incubated with ab2907 (1:75) for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat anti-rabbit IgG secondary antibody (1:250) for 30 minutes at room temperature. Actin was stained with DyLight 550 Phalloidin (1:120) and nuclei (blue) were stained with Hoechst (1ug/ml) for 30 minutes. Images were taken at 20X magnification.
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Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)Immunocytochemistry/Immunofluorescence analysis of HMVEC cells labelling Calreticulin using ab2907.
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Immunocytochemistry/ Immunofluorescence - Anti-Calreticulin antibody - ER Marker (ab2907)This image is courtesy of an anonymous AbreviewImmunofluorescence analysis of HepG2 cells, staining Calreticulin with ab2907.
Cells were fixed with paraformaldehyde, permeabilized with 0.1% Saponin and blocked with 10% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/200 in PBS + 0.1% saponin) for 1 hour at 20°C. An AlexaFluor®647-conjugated donkey anti-rabbit polyclonal IgG (1/400) was used as the secondary antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (229)
ab2907 has been referenced in 229 publications.
- Heshmati Aghda N et al. Dual Photothermal/Chemotherapy of Melanoma Cells with Albumin Nanoparticles Carrying Indocyanine Green and Doxorubicin Leads to Immunogenic Cell Death. Macromol Biosci 22:e2100353 (2022). PubMed: 34762334
- Mao Z et al. KRAS(G12D) can be targeted by potent inhibitors via formation of salt bridge. Cell Discov 8:5 (2022). PubMed: 35075146
- Konda P et al. Photodynamic therapy of melanoma with new, structurally similar, NIR-absorbing ruthenium (II) complexes promotes tumor growth control via distinct hallmarks of immunogenic cell death. Am J Cancer Res 12:210-228 (2022). PubMed: 35141014
- Record M et al. Targeting the liver X receptor with dendrogenin A differentiates tumour cells to secrete immunogenic exosome-enriched vesicles. J Extracell Vesicles 11:e12211 (2022). PubMed: 35411723
- Dinavahi SS et al. Targeting WEE1/AKT Restores p53-Dependent Natural Killer-Cell Activation to Induce Immune Checkpoint Blockade Responses in "Cold" Melanoma. Cancer Immunol Res 10:757-769 (2022). PubMed: 35439317