Recombinant Anti-CAMK1D antibody [EPR3536(2)] (ab172618)
![Western blot - Anti-CAMK1D antibody [EPR3536(2)] (ab172618)](https://www.abcam.com/ps/products/172/ab172618/Images/ab172618-266712-anti-camk1d-antibody-epr35362-western-blot.jpg "Western blot - Anti-CAMK1D antibody [EPR3536(2)] (ab172618)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3536(2)] to CAMK1D
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-CAMK1D antibody [EPR3536(2)]
See all CAMK1D primary antibodies -
Description
Rabbit monoclonal [EPR3536(2)] to CAMK1D -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal forebrain, 293T and HeLa lysates; 293T cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3536(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab172618 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/10000 - 1/50000. Detects a band of approximately 40 kDa (predicted molecular weight: 43 kDa).
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| ICC/IF |
1/50 - 1/100.
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| Notes |
|---|
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WB
1/10000 - 1/50000. Detects a band of approximately 40 kDa (predicted molecular weight: 43 kDa). |
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ICC/IF
1/50 - 1/100. |
Target
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Function
Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. May regulate calcium-mediated granulocyte function. May play a role in apoptosis of erythroleukemia cells. Activates MAP kinase MAPK3 (By similarity). In vitro, phosphorylates transcription factor CREM isoform Beta and probably CREB1. -
Tissue specificity
Broadly expressed. Highly and mostly expressed in polymorphonuclear leukocytes (neutrophilic and eosinophilic granulocytes) while little or no expression is observed in monocytes and lymphocytes. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain. -
Domain
The autoinhibitory domain overlaps with the calmodulin binding region and interacts in the inactive folded state with the catalytic domain as a pseudosubstrate. -
Cellular localization
Cytoplasm. Nucleus. Predominantly cytoplasmic (Probable). Also nuclear upon activation. - Information by UniProt
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Database links
- Entrez Gene: 57118 Human
- Entrez Gene: 227541 Mouse
- Entrez Gene: 307124 Rat
- Omim: 607957 Human
- SwissProt: Q8IU85 Human
- SwissProt: Q8BW96 Mouse
- Unigene: 659517 Human
- Unigene: 191949 Mouse
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Alternative names
- A630059D12Rik antibody
- Calcium/calmodulin dependent protein kinase ID antibody
- Calcium/calmodulin dependent protein kinase type 1D antibody
see all
Images
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Western blot - Anti-CAMK1D antibody [EPR3536(2)] (ab172618)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: CAMK1D knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: HEK293 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab172618 observed at 43 kDa. Red - loading control, ab18058, observed at 124 kDa.ab172618 was shown to specifically react with CAMK1D when CAMK1D knockout samples were used. Wild-type and CAMK1D knockout samples were subjected to SDS-PAGE. ab172618 and ab18058 (loading control to Vinculin) were both diluted 1/10,000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-CAMK1D antibody [EPR3536(2)] (ab172618)](https://www.abcam.com/ps/products/172/ab172618/Images/ab172618-195670-anti-camk1d-antibody-epr35362-western-blot.jpg)
Western blot - Anti-CAMK1D antibody [EPR3536(2)] (ab172618)All lanes : Anti-CAMK1D antibody [EPR3536(2)] (ab172618) at 1/10000 dilution
Lane 1 : Human fetal forebrain tissue lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 43 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-CAMK1D antibody [EPR3536(2)] (ab172618)](https://www.abcam.com/ps/products/172/ab172618/Images/ab172618-195671-anti-camk1d-antibody-epr35362-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CAMK1D antibody [EPR3536(2)] (ab172618)Immunofluorescent analysis of 293T cells labeling CAMK1D with ab172618 at 1/50 dilution (red). DAPI nuclear staining (blue).
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![Anti-CAMK1D antibody [EPR3536(2)] (ab172618)](https://www.abcam.com/ps/products/172/ab172618/Images/ab172618-4-benefits-of-recombinant-antibodies.png)
Anti-CAMK1D antibody [EPR3536(2)] (ab172618)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab172618 has been referenced in 4 publications.
- Liu W & Li S LncRNA ILF3-AS1 Promotes the Progression of Colon Adenocarcinoma Cells Through the miR-619-5p/CAMK1D Axis. Onco Targets Ther 14:1861-1872 (2021). PubMed: 33737811
- Volpin V et al. CAMK1D Triggers Immune Resistance of Human Tumor Cells Refractory to Anti-PD-L1 Treatment. Cancer Immunol Res 8:1163-1179 (2020). PubMed: 32665263
- Fromont C et al. Discovery of Highly Selective Inhibitors of Calmodulin-Dependent Kinases That Restore Insulin Sensitivity in the Diet-Induced Obesity in Vivo Mouse Model. J Med Chem 63:6784-6801 (2020). PubMed: 32433887
- Alfaradhi MZ et al. Maternal Obesity in Pregnancy Developmentally Programs Adipose Tissue Inflammation in Young, Lean Male Mice Offspring. Endocrinology 157:4246-4256 (2016). WB ; Mouse . PubMed: 27583789