Anti-CaMK2 beta gamma delta (phospho T287) antibody (ab182647)
Key features and details
- Rabbit polyclonal to CaMK2 beta gamma delta (phospho T287)
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-CaMK2 beta gamma delta (phospho T287) antibody
See all CaMK2 beta gamma delta primary antibodies -
Description
Rabbit polyclonal to CaMK2 beta gamma delta (phospho T287) -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human CaMK2 beta gamma delta (phospho T287) conjugated to Keyhole Limpet Haemocyanin (KLH). Peptide sequence around phosphorylation site of Threonine 287 (Q-E-T(p)-V-E) derived from Human CaMK2 beta gamma delta. Swiss-Prot#: Q13554/Q13555/Q13557.
Database link: NP_001211.3 -
Positive control
- WB: Rat brain lysate, human 22RV1 cells Immunohistochemistry: Human-liver-cancer tissue, Rat testis tissue, mouse testis tissue Immunocytochemistry: Human lung tissue, rat lung tissue
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 0.88% Sodium chloride, 50% Glycerol
PBS (without Mg2+and Ca2+) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab182647 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab182647 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/200.
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ICC/IF |
1/200.
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WB |
1/500. Predicted molecular weight: 50 kDa.
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Notes |
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IHC-P
1/200. |
ICC/IF
1/200. |
WB
1/500. Predicted molecular weight: 50 kDa. |
Target
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Relevance
The product of this gene belongs to the serine/threonine protein kinase family and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells, the enzyme is composed of four different chains: alpha, beta, gamma, and delta. It is possible that distinct isoforms of this chain have different cellular localizations and interact differently with calmodulin. Eight transcript variants encoding eight distinct isoforms have been identified for this gene. -
Cellular localization
Cytoplasmic and Nuclear -
Database links
- Entrez Gene: 816 Human
- Entrez Gene: 817 Human
- Entrez Gene: 818 Human
- Entrez Gene: 108058 Mouse
- Entrez Gene: 12323 Mouse
- Entrez Gene: 12325 Mouse
- Entrez Gene: 171140 Rat
- Entrez Gene: 24245 Rat
see all -
Alternative names
- Calcium/calmodulin-dependent protein antibody
- CaM kinase II beta subunit antibody
- CaM-kinase II beta chain antibody
see all
Images
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All lanes : Anti-CaMK2 beta gamma delta (phospho T287) antibody (ab182647) at 1/500 dilution
Lane 1 : Rat brain lysate.
Lane 2 : Rat brain lysate with antigen-specific peptide
Predicted band size: 50 kDa -
All lanes : Anti-CaMK2 beta gamma delta (phospho T287) antibody (ab182647)
Lane 1 : Rat Brain
Lane 2 : Rat brain blocked with phospho peptide
Predicted band size: 50 kDa -
Anti-CaMK2 beta gamma delta (phospho T287) antibody (ab182647) at 1/500 dilution + Human 22RV1 cells
Predicted band size: 50 kDa -
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue labelling CaMK2 beta gamma delta (phospho T287) with ab182647 at 1/200 and incubated overnight at 4°C followed by a secondary antibody diluted at 1/200, incubated at RT for 30 minutes.
Negative control: no staining on human live cancer tissue.
Negative control: Secondary antibody only.
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0) for 20 minutes.
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Immunohistochemical analysis of paraffin-embedded Rat testis tissue labelling CaMK2 beta gamma delta (phospho T287) with ab182647 at 1/200 and incubated overnight at 4°C followed by a secondary antibody diluted at 1/200, incubated at RT for 30 minutes. Negative control: no staining on Rat-testis tissue.
Negative control: Secondary antibody only.
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0) for 20 minutes.
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Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labelling CaMK2 beta gamma delta (phospho T287) with ab182647 at 1/200 and incubated overnight at 4°C followed by a secondary antibody diluted at 1/200, incubated at RT for 30 minutes. Negative control: no staining on Rat-testis tissue.
Negative control: Secondary antibody only.
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0) for 20 minutes.
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Immunofluorescent analysis of human lung tissue labelling CaMK2 beta gamma delta (phospho T287) with ab182647 at 1/200 dilution, followed by Cy3 labelled secondary antibody 1/300. The Nuclear counterstain was DAPI (Blue).
Left: ab182647, Middle: DAPI, Right: Merged
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Immunofluorescent analysis of rat lung tissue labelling CaMK2 beta gamma delta (phospho T287) with ab182647 at 1/200 dilution, followed by Cy3 labelled secondary antibody 1/300. The Nuclear counterstain was DAPI (Blue). Left: ab182647, Middle: DAPI, Right: Merged
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab182647 has been referenced in 8 publications.
- Prakash O et al. Calmodulin variant E140G associated with long QT syndrome impairs CaMKIIδ autophosphorylation and L-type calcium channel inactivation. J Biol Chem 299:102777 (2023). PubMed: 36496072
- Qian D et al. Trans-cinnamaldehyde protects against phenylephrine-induced cardiomyocyte hypertrophy through the CaMKII/ERK pathway. BMC Complement Med Ther 22:115 (2022). PubMed: 35468773
- Liu YS et al. Calreticulin nuclear translocalization alleviates CaM/CaMKII/CREB signaling pathway to enhance chemosensitivity in HDAC inhibitor-resistant hepatocellular carcinoma cells. Aging (Albany NY) 14:5097-5115 (2022). PubMed: 35724265
- Eigler T et al. ERK1/2 inhibition promotes robust myotube growth via CaMKII activation resulting in myoblast-to-myotube fusion. Dev Cell 56:3349-3363.e6 (2021). PubMed: 34932950
- Abuna RPF et al. Frizzled 6 disruption suppresses osteoblast differentiation induced by nanotopography through the canonical Wnt signaling pathway. J Cell Physiol 235:8293-8303 (2020). PubMed: 32239701
- Yifa O et al. The small molecule Chicago Sky Blue promotes heart repair following myocardial infarction in mice. JCI Insight 4:N/A (2019). PubMed: 31723055
- Zou S et al. Effects of HIV-1 Tat on oligodendrocyte viability are mediated by CaMKIIß-GSK3ß interactions. J Neurochem 149:98-110 (2019). PubMed: 30674062
- Wu Y et al. Cardiac Protection of Valsartan on Juvenile Rats with Heart Failure by Inhibiting Activity of CaMKII via Attenuating Phosphorylation. Biomed Res Int 2017:4150158 (2017). PubMed: 28536695