Anti-Cannabinoid Receptor I antibody (ab137410)
Key features and details
- Rabbit polyclonal to Cannabinoid Receptor I
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Cannabinoid Receptor I antibody
See all Cannabinoid Receptor I primary antibodies -
Description
Rabbit polyclonal to Cannabinoid Receptor I -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide, corresponding to a region within internal sequence amino acids 270-365 of Human Cannabinoid Receptor I
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Positive control
- Human Cannabinoid Receptor I transfected HEK-293T cells. U87-MG, mouse brain and Rat brain whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137410 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 53 kDa.
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| IHC-P |
1/100 - 1/1000. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
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| Notes |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 53 kDa. |
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IHC-P
1/100 - 1/1000. Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
Target
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Function
Involved in cannabinoid-induced CNS effects. Acts by inhibiting adenylate cyclase. Could be a receptor for anandamide. Inhibits L-type Ca(2+) channel current. Isoform 2 and isoform 3 have altered ligand binding. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1268 Human
- Entrez Gene: 12801 Mouse
- Entrez Gene: 25248 Rat
- Omim: 114610 Human
- SwissProt: P21554 Human
- SwissProt: P47746 Mouse
- SwissProt: P20272 Rat
- Unigene: 709067 Human
see all -
Alternative names
- CANN6 antibody
- Cannabinoid receptor 1 antibody
- CB-R antibody
see all
Images
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Western blot - Anti-Cannabinoid Receptor I antibody (ab137410)Anti-Cannabinoid Receptor I antibody (ab137410) at 1/5000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 53 kDa
7.5% SDS PAGE -
Western blot - Anti-Cannabinoid Receptor I antibody (ab137410)Anti-Cannabinoid Receptor I antibody (ab137410) at 1/5000 dilution + Rat brain whole cell lysate at 50 µg
Predicted band size: 53 kDa
7.5% SDS PAGE -
Western blot - Anti-Cannabinoid Receptor I antibody (ab137410)All lanes : Anti-Cannabinoid Receptor I antibody (ab137410) at 1/1000 dilution
Lane 1 : HEK 293T whole cell lysate
Lane 2 : HEK 293T whole cell lysate transfected with Cannabinoid Receptor I
Lysates/proteins at 10 µg per lane.
Predicted band size: 53 kDa
10 % SDS-PAGE -
Western blot - Anti-Cannabinoid Receptor I antibody (ab137410)Anti-Cannabinoid Receptor I antibody (ab137410) at 1/1000 dilution + U87-MG whole cell lysate at 30 µg
Predicted band size: 53 kDa
10 % SDS-PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cannabinoid Receptor I antibody (ab137410)Immunohistochemical analysis of paraffin-embedded OVCA, labelling Cannabinoid Receptor I using ab137410 at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab137410 has been referenced in 2 publications.
- Sugimoto N et al. Corticosterone inhibits the expression of cannabinoid receptor-1 and cannabinoid receptor agonist-induced decrease in cell viability in glioblastoma cells. Oncol Lett 18:1557-1563 (2019). PubMed: 31423223
- Yu J et al. Phosphorylated CRMP2 Regulates Spinal Nociceptive Neurotransmission. Mol Neurobiol N/A:N/A (2018). PubMed: 30565051