Recombinant Anti-Cannabinoid Receptor I (phospho S316) antibody [EPR2223(N)] (ab186428)
![Western blot - Anti-Cannabinoid Receptor I (phospho S316) antibody [EPR2223(N)] (ab186428)](https://www.abcam.com/ps/products/186/ab186428/Images/ab186428-219963-anti-cannabinoid-receptor-i-phospho-s316-antibody-epr2223n-western-blot.jpg "Western blot - Anti-Cannabinoid Receptor I (phospho S316) antibody [EPR2223(N)] (ab186428)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2223(N)] to Cannabinoid Receptor I (phospho S316)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Cannabinoid Receptor I (phospho S316) antibody [EPR2223(N)]
See all Cannabinoid Receptor I primary antibodies -
Description
Rabbit monoclonal [EPR2223(N)] to Cannabinoid Receptor I (phospho S316) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt (Intra) -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal brain lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2223(N) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab186428 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000. Detects a band of approximately 46, 53 kDa (predicted molecular weight: 53 kDa).
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| Notes |
|---|
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WB
1/1000. Detects a band of approximately 46, 53 kDa (predicted molecular weight: 53 kDa). |
Application notes
Is unsuitable for Flow Cyt (Intra).
Target
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Function
Involved in cannabinoid-induced CNS effects. Acts by inhibiting adenylate cyclase. Could be a receptor for anandamide. Inhibits L-type Ca(2+) channel current. Isoform 2 and isoform 3 have altered ligand binding. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 1268 Human
- Omim: 114610 Human
- SwissProt: P21554 Human
- Unigene: 709067 Human
- Unigene: 75110 Human
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Alternative names
- CANN6 antibody
- Cannabinoid receptor 1 antibody
- CB-R antibody
see all
Images
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Western blot - Anti-Cannabinoid Receptor I (phospho S316) antibody [EPR2223(N)] (ab186428)All lanes : Anti-Cannabinoid Receptor I (phospho S316) antibody [EPR2223(N)] (ab186428) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal brain lysate with Lambda phosphatase
Lysates/proteins at 1/10 dilution per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 53 kDa
Observed band size: 53 kDa
Additional bands at: 46 kDa (possible isoform)Based on the sequence analysis, ab186428 recognizes three isoforms with the predicted MWs of 53KDa, 46KDa and 49KDa, respectively.
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![Anti-Cannabinoid Receptor I (phospho S316) antibody [EPR2223(N)] (ab186428)](https://www.abcam.com/ps/products/186/ab186428/Images/ab186428-3-benefits-of-recombinant-antibodies.png)
Anti-Cannabinoid Receptor I (phospho S316) antibody [EPR2223(N)] (ab186428)
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab186428 has been referenced in 2 publications.
- Rodríguez-Manzo G et al. Endocannabinoids Released in the Ventral Tegmental Area During Copulation to Satiety Modulate Changes in Glutamate Receptors Associated With Synaptic Plasticity Processes. Front Synaptic Neurosci 13:701290 (2021). WB ; Rat . PubMed: 34483875
- Sánchez-Pastor E et al. Type 2 Diabetes Alters Vascular Cannabinoid Receptor 1 Expression, Phosphorylation Status, and Vasorelaxation in Rat Aorta. Molecules 25:N/A (2020). PubMed: 33114620