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  1. Link

    products/primary-antibodies/cannabinoid-receptor-ii-antibody-ab3561.pdf

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Neuroscience Neurotransmission Receptors / Channels GPCR Cannabinoid Receptor
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Anti-Cannabinoid Receptor II antibody (ab3561)

  • Datasheet
  • SDS
Reviews (18)Q&A (15)References (63)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cannabinoid Receptor II antibody (ab3561)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cannabinoid Receptor II antibody (ab3561)
  • Western blot - Anti-Cannabinoid Receptor II antibody (ab3561)
  • Immunocytochemistry - Anti-Cannabinoid Receptor II antibody (ab3561)

Key features and details

  • Rabbit polyclonal to Cannabinoid Receptor II
  • Suitable for: ICC, WB, IHC-P
  • Reacts with: Rat, Human, Recombinant fragment
  • Isotype: IgG

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Overview

  • Product name

    Anti-Cannabinoid Receptor II antibody
    See all Cannabinoid Receptor II primary antibodies
  • Description

    Rabbit polyclonal to Cannabinoid Receptor II
  • Host species

    Rabbit
  • Specificity

    We have had mixed results for use of this antibody in mouse. Thus, we are removing mouse as a guaranteed application and welcome any feedback from customers who have used this antibody in mouse.

  • Tested applications

    Suitable for: ICC, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human, Recombinant fragment
  • Immunogen

    Recombinant fragment within Rat Cannabinoid Receptor II aa 1-100 (N terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: Q9QZN9

  • Positive control

    • WB: HT29, C6, rat colon cell lysate; IHC: human tonsil tissue, skin tissue; ICC: AtT20 cells transfected with rat CB2 gene
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 50% Glycerol, 0.1% BSA, 49% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Primary antibody notes

    Cannabinoids exert their well known physiological effects through two G protein coupled receptors, cannabinoid receptor 1 (CB1) and CB2. Both cannabinoid receptors have been shown to inhibit adenylyl cyclase as well as stimulate the mitogen-activated protein kinase, MAPK. CB1 receptors also modulate ion channels through direct G-protein interactions. Delta 9-tetrahydrocannibinol and related ligands likely exert their psychoactive effects by inhibiting presynaptic N- and P / Q type calcium channels. CB2 is thought to function primarily in the immune system although it has been suggested to be present in the central nervous system, including the retina.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • Cannabinoid Receptor
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Cannabinoid Receptor II protein (ab114225)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab3561 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC
1/500.
WB (4)
1/50 - 1/500. Predicted molecular weight: 40 kDa.
IHC-P (2)
1/10 - 1/100.
Notes
ICC
1/500.
WB
1/50 - 1/500. Predicted molecular weight: 40 kDa.
IHC-P
1/10 - 1/100.

Target

  • Function

    Heterotrimeric G protein-coupled receptor for endocannabinoid 2-arachidonoylglycerol mediating inhibition of adenylate cyclase. May function in inflammatory response, nociceptive transmission and bone homeostasis.
  • Tissue specificity

    Preferentially expressed in cells of the immune system with higher expression in B cells and NK cells (at protein level). Expressed in skin in suprabasal layers and hair follicles (at protein level). Highly expressed in tonsil and to a lower extent in spleen, peripheral blood mononuclear cells, and thymus. PubMed:14657172 could not detect expression in normal brain. Expressed in brain by perivascular microglial cells and dorsal root glanglion sensory neurons (at protein level).
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Post-translational
    modifications

    Constitutively phosphorylated on Ser-352; phosphorylation increases cell internalization and desensitizes the receptor.
  • Cellular localization

    Cell membrane. Cell projection > dendrite. Perikaryon. Localizes to apical dendrite of pyramidal neurons.
  • Target information above from: UniProt accession P34972 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1269 Human
    • Entrez Gene: 57302 Rat
    • Omim: 605051 Human
    • SwissProt: P34972 Human
    • SwissProt: Q9QZN9 Rat
    • Unigene: 73037 Human
    • Unigene: 48776 Rat
    • Alternative names

      • Cannabinoid receptor 2 antibody
      • Cannabinoid receptor 2 macrophage antibody
      • CB 2 antibody
      • CB-2 antibody
      • CB2 antibody
      • CNR2 antibody
      • CNR2_HUMAN antibody
      • CNRII antibody
      • CX 5 antibody
      • CX5 antibody
      • hCB2 antibody
      • testis-dominant CNR2 isoform CB2 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cannabinoid Receptor II antibody (ab3561)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cannabinoid Receptor II antibody (ab3561)

      ab3561 labelling Cannabinoid Receptor II in the cytoplasm of Human tonsil tissue (right) compared with a negative control (left) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tissue sections were incubated with the primary antibody (1:20 in 3% BSA-PBS) overnight at 4°C. A anti-rabbit HRP was used as the secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cannabinoid Receptor II antibody (ab3561)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cannabinoid Receptor II antibody (ab3561)

      ab3561 labelling Cannabinoid Receptor II in the cytoplasm of Human skin tissue (right) compared with a negative control (left) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were blocked in 3% H2O2-methanol for 15 min at room temperature. Tssiue sections were incubated with the primary antibody (1:20 in 3% BSA-PBS) overnight at 4°C. A anti-rabbit HRP was used as the secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

    • Western blot - Anti-Cannabinoid Receptor II antibody (ab3561)
      Western blot - Anti-Cannabinoid Receptor II antibody (ab3561)
      All lanes : Anti-Cannabinoid Receptor II antibody (ab3561) at 1/200 dilution

      Lane 1 : HT29 cell lysate
      Lane 2 : C6 cell lysate
      Lane 3 : Rat colon cell lysate

      Lysates/proteins at 25 µg per lane.

      Predicted band size: 40 kDa
      Observed band size: 40 kDa

    • Immunocytochemistry - Anti-Cannabinoid Receptor II antibody (ab3561)
      Immunocytochemistry - Anti-Cannabinoid Receptor II antibody (ab3561)

      Immunocytochemistry/immunofluorescence analysis of AtT20 cells transfect with the rat CB2 gene labeling Cannabinoid Receptor II with ab3561.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (63)

    Publishing research using ab3561? Please let us know so that we can cite the reference in this datasheet.

    ab3561 has been referenced in 63 publications.

    • Miranzadeh Mahabadi H  et al. Cannabinoid receptors distribution in mouse cortical plasma membrane compartments. Mol Brain 14:89 (2021). PubMed: 34099009
    • Rivera P  et al. Differential hepatoprotective role of the cannabinoid CB1 and CB2 receptors in paracetamol-induced liver injury. Br J Pharmacol 177:3309-3326 (2020). PubMed: 32167157
    • Remiszewski P  et al. Chronic Cannabidiol Administration Fails to Diminish Blood Pressure in Rats with Primary and Secondary Hypertension Despite Its Effects on Cardiac and Plasma Endocannabinoid System, Oxidative Stress and Lipid Metabolism. Int J Mol Sci 21:N/A (2020). PubMed: 32075117
    • Gupta B  et al. Anti-apoptotic and Immunomodulatory Effect of CB2 Agonist, JWH133, in a Neonatal Rat Model of Hypoxic-Ischemic Encephalopathy. Front Pediatr 8:65 (2020). PubMed: 32175293
    • Huang W  et al. NOX2 is involved in CB2-mediated protection against lung ischemia-reperfusion injury in mice. Int J Clin Exp Pathol 13:277-285 (2020). PubMed: 32211110
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 33 Abreviews or Q&A

    Immunohistochemistry free floating abreview for Anti-Cannabinoid Receptor II antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Mouse Cell (Brain)
    Specification
    Brain
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jan 25 2022

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cannabinoid Receptor II antibody

    Inconclusive
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rhesus monkey Tissue sections (pituitary, skin, adrenal)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
    Permeabilization
    No
    Specification
    pituitary, skin, adrenal
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 07 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cannabinoid Receptor II antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HT29 cell line (colon cancer epithelial cell line))
    Permeabilization
    Yes - Tween 0,25%
    Specification
    HT29 cell line (colon cancer epithelial cell line)
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
    Fixative
    Methanol
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 30 2015

    Western blot abreview for Anti-Cannabinoid Receptor II antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (bladder)
    Loading amount
    100 µg
    Specification
    bladder
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (10% gel)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Eun-Young Park

    Verified customer

    Submitted Apr 09 2013

    Question

    *
    The order #: *is for ab3561 which we received on *The order number for the first vial is *. Thanks.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 27 2012

    Answer

    Thank you for that clarification.

    I have sent you a FedEx link that you can complete to be able to return the vial of ab3561 from order number *. Once that order is returned to us, we will credit the cost of that product back to you. The credit note for this is at the bottom of the email.

    As for the vial that you recieved on order number *, as we discussed I am sending you a vial of ab3560. The order number for this is 1* and you should have it on Tueday morning.

    If there is anything else I can help you with, please let me know.

    Credit Note for order #*

    Your credit note ID is *.

    I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.

    Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

    The credit note ID is for your reference only and does not automatically guarantee the credit.

    I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

    Read More

    Abcam Scientific Support

    Answered on Oct 27 2012

    Question

    The order ID for ab3561 is *. Let me know if you need anything else.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 26 2012

    Answer

    Thank you for that information.

    That order number (*), is for the vial of ab3561 that you just received today, correct? If so, I will go ahead and set up the return and credit for this order.

    Do you have the order number for the first vial of ab3561 that you received, as I will need that number to send the free of charge replacement.

    Sorry, I do not mean to make this complicated; I just want to make sure I replace/refund the correct orders.

    Thank you for your patience and cooperation and I hope to have this resolved quickly.

    Read More

    Abcam Scientific Support

    Answered on Oct 26 2012

    Question

    Thanks for your reply regarding product #ab3561 (Anti-Cannabinoid Receptor II antibody). We tried several cell-line known to express CB2 (i.e. MDA-231, Jurkat) and tried several times using it in flow cytometry experiments with various range of dilution and it just seems that this antibody does not show binding. We are very frustrated and would like to ask if you could perhaps recommend some other antibodies that you carry that you could recommend and send to us ASAP: I found these two that show they can be used for flow cytometry

    https://www.abcam.com/cannabinoid-receptor-ii-antibody-ab3560.html

    https://www.abcam.com/cannabinoid-receptor-ii-antibody-ab45942.html

    We have already ordered another tube of #ab3561 which we have recieved today; but based on our results today, I don't think this product will be of any use. Please let me know as soon as you can. Thanks.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 26 2012

    Answer

    Thank you for your reply.

    I am sorry about the frustration you are having with ab3561 in flow cytometry and I would be happy to replace the first vial that you received. Of the two possible replacements you mentioned in you previous email, we only guarantee ab3560 to work in flow cytometry, although we have only tested it in rat and human samples. I know you were transfecting CHO cells, but is the construct for rat or human?

    For the vial of ab3561 that you just received, if you could please provide the order number for this product, I will make arrangements for the cost to be credited back to your account and also for you to return that vial to Abcam.

    I look forward to your reply and helping to resolve this issue.

    Read More

    Abcam Scientific Support

    Answered on Oct 26 2012

    Question

    Hi- we bought your product #ab3561 (Anti-Cannabinoid Receptor II antibody) and we are using it in flow cytometry to check binding to CHOK1 cells expressing CB2 via transfection: the product insert mentions dilutions used based on assay being developed and does not really gove a range to use. We have been using 10ug/ml and have not been seeing much binding. Is there a range of dilution you could recommend for our application?? thanks.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 25 2012

    Answer

    Thank you for contacting Abcam.

    The antibody is covered under our Abpromise for six months and is guaranteed to work in flow cytometry on a number of samples. If we cannot resolve the issue you are having with the antibody then I would be happy to either send a replacement antibody or to process a refund.

    The information we have regarding concentrations for the antibody working in flow cytometry, come from some references, which are available on the website. That information is also below:

    http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=20206138&dopt=Abstract - They used 1/100 dilution.
    http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=18239598&dopt=Abstract - They do not specify the concentration they used.



    If you cannot find any protocol information in these references that prove to be useful, then please feel free to send me a copy of the protocol and results that you are getting and I would be happy to look them over and see if there is anything that I can suggest.

    Please let me know if there is anything else I can help you with.

    Read More

    Abcam Scientific Support

    Answered on Oct 25 2012

    Question

    Dear ,
    I would like to purchase the following products:
    - cod. ab3561 - Anti-Cannabinoid Receptor II antibody;
    - cod. ab80437 - EXPOSE Rabbit specific HRP/DAB detection IHC kit (how much slides can I stain with this 15mL kit?);
    - cod. ab94726 - EXPOSE Rabbit specific HRP/DAB detection IHC kit;
    - cod. ab64026 - 25x PBS Buffer pH 7.6;
    - cod. ab64214 - 10x Citrate Buffer pH 6.0;
    Furthermore, I have serveral question to ask you:
    - Is the selected Expose detection kit suitablen for the binding with primary antibody "ab3561"?
    - which tissue slides could I select as positive and negative control?
    - have abcam a primay antibody enhancer, and could it be useful to the detection?
    - could you inform me if these products are or are not in stock, please?
    As I said to you in the previous email, I need a quotation for all these products with the indication of the code, the full prize and the 5% reduced price. Then I'll deliver the quotation and the indication of selected objects to the center, which will request you the proforma invoice sending you all the informations about the billing and the shipping address.
    I hope I was clear enough, otherwise please do not hesitate to contact me for further clarifications.
    Best regards

    Read More

    Abcam community

    Verified customer

    Asked on Oct 18 2012

    Answer

    Thank you for contacting us.

    The number of slides that could be used with this kit depends on the tissue sections, and the volume of reagents per slide. The volume used should be enough to cover them. We normally use 200ul per slide in house, but 100ul should be enough. If 100ul are used then you could run 150 slides with the reagents provided.

    This kit is compatible with any primary antibody suitable for IHC in paraffin sections, as long as the recommendations and protocols are followed.

    Regarding the tissue slides to use as controls, I checked the http://www.proteinatlas.org/ENSG00000188822, which suggests digestive tract, female tissues or kidney as positive controls. Other tissues could be Human Spinal Cord, Rat Spinal Cord, and Mouse Thymus. I can check if you are interested what would be a good negative control with my lab colleagues.

    If you are using the EXPOSE kit there is no need to use an enhancer for detection.

    Except for the products ab94726 and ab64026, the rest of them are currently in stock, and you will receive them within two working days.

    Please note all of our products are covered by our Abpromise® guarantee, which ensures that you can trust our products, and they should work in the tested species and applications stated on the datasheet, or we will offer a replacement, credit, or refund, if reported within 6 months of purchase.

    To read about our Abpromise policy: https://www.abcam.com/index.html?pageconfig=abpromise

    You will receive the proforma invoice with the requested products shortly.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Oct 18 2012

    Question


    Here is the information that you requested along with some of our data. Please feel free to let me know if you need more information.
    Thank you,

    Read More

    Abcam community

    Verified customer

    Asked on Aug 29 2012

    Answer

    Thank you very much for your reply and for sending the additional information.

    Your credit note ID is ***

    I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department at mailto:us.credits@abcam.com so that we may gather the correct information needed for the refund. Please refer to the credit note ID in any correspondence with our accounting department.

    I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further assistance or advice.

    Read More

    Abcam Scientific Support

    Answered on Aug 29 2012

    1-10 of 33 Abreviews or Q&A

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