Anti-Carbonic Anhydrase 9/CA9 antibody (ab15086)
Key features and details
- Rabbit polyclonal to Carbonic Anhydrase 9/CA9
- Suitable for: Flow Cyt (Intra), ICC, IHC-Fr, IHC-P, WB
- Reacts with: Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Carbonic Anhydrase 9/CA9 antibody
See all Carbonic Anhydrase 9/CA9 primary antibodies -
Description
Rabbit polyclonal to Carbonic Anhydrase 9/CA9 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), ICC, IHC-Fr, IHC-P, WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
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Positive control
- WB: Ramos, A549, HeLa and MDA-MB-231 cell lysates; Rat renal cortex lysate. Intr Flow Cyt: A-431 and U-87 MG cells. ICC: A-431 cells. IHC-Fr: Human renal cell cancer tumor sections. IHC-P: Human breast cancer sections.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Immunizing Peptide (Blocking)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab15086 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/1000.
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| ICC |
Use a concentration of 2 - 5 µg/ml.
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| IHC-Fr | (1) |
1/200 - 1/500.
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| IHC-P | (2) |
1/200 - 1/500.
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| WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 49.7 kDa).Can be blocked with Recombinant Human RND1 protein (ab109840).
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| Notes |
|---|
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Flow Cyt (Intra)
1/1000. |
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ICC
Use a concentration of 2 - 5 µg/ml. |
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IHC-Fr
1/200 - 1/500. |
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IHC-P
1/200 - 1/500. |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 49.7 kDa).Can be blocked with Recombinant Human RND1 protein (ab109840). |
Target
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Function
Reversible hydration of carbon dioxide. Participates in pH regulation. May be involved in the control of cell proliferation and transformation. Appears to be a novel specific biomarker for a cervical neoplasia. -
Tissue specificity
Expressed primarily in carcinoma cells lines. Expression is restricted to very few normal tissues and the most abundant expression is found in the epithelial cells of gastric mucosa. -
Sequence similarities
Belongs to the alpha-carbonic anhydrase family.
Contains 1 alpha-carbonic anhydrase domain. -
Post-translational
modificationsAsn-346 bears high-mannose type glycan structures. -
Cellular localization
Nucleus. Nucleus, nucleolus. Cell membrane. Cell projection, microvillus membrane. Found on the surface microvilli and in the nucleus, particularly in nucleolus. - Information by UniProt
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Database links
- Entrez Gene: 768 Human
- Entrez Gene: 313495 Rat
- Omim: 603179 Human
- SwissProt: Q16790 Human
- Unigene: 63287 Human
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Alternative names
- CA-IX antibody
- CA9 antibody
- CAH9_HUMAN antibody
see all
Images
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Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody (ab15086)Western blot analysis labeling Carbonic Anhydrase 9/CA9 with ab15086 at 1 ug/ml in rat renal cortex lysate.
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Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody (ab15086)Western blot analysis labeling Carbonic Anhydrase 9/CA9 with ab15086 at 1 ug/ml in 1) HeLa, 2) MDA-MB-231, and 3) A549 whole cell lysates. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Carbonic Anhydrase 9/CA9 antibody (ab15086)Immunohistochemistry analysis of a formalin-fixed, paraffin-embedded tissue section of human breast cancer using ab15086 at 1:1000 dilution. The primary antibody bound to Carbonic Anydrase 9/CA9 antigens in the tissue section was detected using a HRP labeled secondary antibody and DAB reagent. Nuclei of the cells were counterstained with hematoxylin. This antibody generated an expected cytoplasmic staining of Carbonic Anydrase 9/CA9 protein with an intense signal around the cellular membranes in tumor cores. The latter are more likely to be hypoxic in growing tumors which signifies that the observed Carbonic Anydrase 9/CA9 staining is specific. -
Immunohistochemistry (Frozen sections) - Anti-Carbonic Anhydrase 9/CA9 antibody (ab15086)Immunohistochemistry analysis of human renal cell cancer tumor cryosections stained with ab15086 at a 1/500 dilution (left) or normal rabbit serum (right). -
Western blot - Anti-Carbonic Anhydrase 9/CA9 antibody (ab15086)All lanes : Anti-Carbonic Anhydrase 9/CA9 antibody (ab15086) at 1 µg/ml
Lane 1 : Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 49.7 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (134)
ab15086 has been referenced in 134 publications.
- Zatovicova M et al. ADAM10 mediates shedding of carbonic anhydrase IX ectodomain non‑redundantly to ADAM17. Oncol Rep 49:N/A (2023). PubMed: 36524367
- Hamon P et al. TGFβ receptor inhibition unleashes interferon-β production by tumor-associated macrophages and enhances radiotherapy efficacy. J Immunother Cancer 10:N/A (2022). PubMed: 35301235
- Lu C et al. Hypoxia-activated neuropeptide Y/Y5 receptor/RhoA pathway triggers chromosomal instability and bone metastasis in Ewing sarcoma. Nat Commun 13:2323 (2022). PubMed: 35484119
- Gombodorj N et al. Effects of Ultrafine Single-Nanometer Oxygen Bubbles on Radiation Sensitivity in a Tumor-Bearing Mouse Model. Int J Mol Sci 23:N/A (2022). PubMed: 35743281
- Takei J et al. Impact of Neoadjuvant Bevacizumab on Neuroradiographic Response and Histological Findings Related to Tumor Stemness and the Hypoxic Tumor Microenvironment in Glioblastoma: Paired Comparison Between Newly Diagnosed and Recurrent Glioblastomas. Front Oncol 12:898614 (2022). PubMed: 35785200