Anti-Carboxypeptidase Y antibody [10A5B5] (ab113685)
![Western blot - Anti-Carboxypeptidase Y antibody [10A5B5] (ab113685)](https://www.abcam.com/ps/products/113/ab113685/Images/ab113685-126379-anti-carboxypeptidase-y-antibody-10a5b5-western-blot.jpg "Western blot - Anti-Carboxypeptidase Y antibody [10A5B5] (ab113685)")
Key features and details
- Mouse monoclonal [10A5B5] to Carboxypeptidase Y
- Suitable for: WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG1
Overview
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Product name
Anti-Carboxypeptidase Y antibody [10A5B5]
See all Carboxypeptidase Y primary antibodies -
Description
Mouse monoclonal [10A5B5] to Carboxypeptidase Y -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Saccharomyces cerevisiae -
Immunogen
Full length native protein (purified). This information is considered to be commercially sensitive.
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Positive control
- Yeast membranes
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purity
Proprietary Purification -
Purification notes
Near homogeneity (Purity >95% by SDS-PAGE). The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by chemical fractionation. -
Clonality
Monoclonal -
Clone number
10A5B5 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab113685 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.5 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa).
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| Notes |
|---|
|
WB
Use a concentration of 0.5 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 59 kDa). |
Target
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Function
Involved in degradation of small peptides. Digests preferentially peptides containing an aliphatic or hydrophobic residue in P1' position, as well as methionine, leucine or phenylalanine in P1 position of ester substrate. -
Sequence similarities
Belongs to the peptidase S10 family. -
Post-translational
modificationsEnters the endoplasmic reticulum as an inactive zymogen and is modified by four N-linked core oligosaccharides, giving rise to a precursor known as P1 (67 kDa). As P1 transits through the Golgi, extension of its core oligosaccharides leads to the Golgi-modified P2 precursor (69 kDa). P2 is sorted away from secretory proteins at or beyond a late Golgi compartment and is subsequently delivered to the vacuole via a prevacuolar endosome-like compartment. Upon arrival in the vacuole, the N-terminal prosegment of P2 is cleaved to yield the enzymatically active mature vacuolar form of CPY (61 kDa). -
Cellular localization
Vacuole. Lysosome-like vacuoles. - Information by UniProt
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Database links
- Entrez Gene: 855343 Saccharomyces cerevisiae
- SwissProt: P00729 Saccharomyces cerevisiae
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Alternative names
- carboxypeptidase C PRC1 antibody
- Carboxypeptidase Y antibody
- Carboxypeptidase YSCY antibody
see all
Images
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Western blot - Anti-Carboxypeptidase Y antibody [10A5B5] (ab113685)All lanes : Anti-Carboxypeptidase Y antibody [10A5B5] (ab113685)
Lane 1 : Yeast whole cell lysate
Lane 2 : Yeast membrane preparation
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 59 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab113685 has been referenced in 7 publications.
- Sun D et al. The cryo-EM structure of the SNX-BAR Mvp1 tetramer. Nat Commun 11:1506 (2020). PubMed: 32198400
- Yanguas F et al. Ent3 and GGA adaptors facilitate diverse anterograde and retrograde trafficking events to and from the prevacuolar endosome. Sci Rep 9:10747 (2019). PubMed: 31341193
- Aviram N et al. The SND proteins constitute an alternative targeting route to the endoplasmic reticulum. Nature 540:134-138 (2016). PubMed: 27905431
- Ast T et al. A cytosolic degradation pathway, prERAD, monitors pre-inserted secretory pathway proteins. J Cell Sci 127:3017-23 (2014). WB ; Saccharomyces cerevisiae . PubMed: 24849653
- Manandhar SP et al. Saccharomyces cerevisiae Env7 is a novel serine/threonine kinase 16-related protein kinase and negatively regulates organelle fusion at the lysosomal vacuole. Mol Cell Biol 33:526-42 (2013). PubMed: 23166297
- Chen J et al. Accumulation of an antidepressant in vesiculogenic membranes of yeast cells triggers autophagy. PLoS One 7:e34024 (2012). PubMed: 22529904
- Li L et al. A Role for Iron-Sulfur Clusters in the Regulation of Transcription Factor Yap5-dependent High Iron Transcriptional Responses in Yeast. J Biol Chem 287:35709-21 (2012). WB ; Saccharomyces cerevisiae . PubMed: 22915593