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RecombinantRabMAb

Recombinant Anti-CARD9 antibody [EPR6489] (ab133560)

Submit a review Q&A (1)References (2)
Western blot - Anti-CARD9 antibody [EPR6489] (ab133560)
  • Western blot - Anti-CARD9 antibody [EPR6489] (ab133560)
  • Western blot - Anti-CARD9 antibody [EPR6489] (ab133560)
  • OI-RD Scanning - Anti-CARD9 antibody [EPR6489] (ab133560)
  • Anti-CARD9 antibody [EPR6489] (ab133560)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6489] to CARD9
  • Suitable for: WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-CARD9 antibody [EPR6489]
    See all CARD9 primary antibodies

  • Description

    Rabbit monoclonal [EPR6489] to CARD9

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human CARD9 aa 450-550. The exact sequence is proprietary.

  • Positive control

    • WB: K562, U937, HL60, HepG2, and THP1 cell lysates

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Dissociation constant (KD)

    KD = 2.12 x 10 -11 M
    Learn more about KD

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR6489

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 62 kDa.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 62 kDa.
Application notes
Is unsuitable for ICC/IF,IHC-P or IP.

Target

  • Function

    Activates NF-kappa-B via BCL10.

  • Tissue specificity

    Highly expressed in spleen. Also detected in liver, placenta, lung, peripheral blood leukocytes and in brain.

  • Involvement in disease

    Defects in CARD9 are the cause of familial candidiasis type 2 (CANDF2) [MIM:212050]. Chronic mucocutaneous candidiasis is characterized by impaired clearance of fungal infections and results in colonization and infections of the mucosa or skin, predominantly with Candida albicans. CANDF2 is an autosomal recessive chronic mucocutaneous candidiasis.

  • Sequence similarities

    Contains 1 CARD domain.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    • Alternative names

      • CANDF2 antibody
      • CARD9 antibody
      • CARD9_HUMAN antibody
      • Caspase recruitment domain family member 9 antibody
      • Caspase recruitment domain-containing protein 9 antibody
      • hCARD9 antibody
      see all

    Images

    • Western blot - Anti-CARD9 antibody [EPR6489] (ab133560)
      Western blot - Anti-CARD9 antibody [EPR6489] (ab133560)
      All lanes : Anti-CARD9 antibody [EPR6489] (ab133560) at 1/1000 dilution

      Lane 1 : THP-1(Human monocytic leukemia monocyte) whole cell lysate
      Lane 2 : 293T (Human embryonic kidney epithelial cell) whole cell lysate
      Lane 3 : A549 (Human lung carcinoma epithelial cell) whole cell lysate
      Lane 4 : NCI-H1299 (Human lung carcinoma epithelial cell) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 62 kDa
      Observed band size: 52-62 kDa why is the actual band size different from the predicted?


      Exposure time: 5 seconds


      Blocking buffer and concentration: 5% NFDM/TBST 

      Diluting buffer and concentration: 5% NFDM/TBST

      293T, A549 and NCI-H1299 are low expressors (PMID:?32164050, PMID:?33558980).

    • Western blot - Anti-CARD9 antibody [EPR6489] (ab133560)
      Western blot - Anti-CARD9 antibody [EPR6489] (ab133560)
      All lanes : Anti-CARD9 antibody [EPR6489] (ab133560) at 1/5000 dilution (Purified)

      Lane 1 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates
      Lane 2 : THP-1 (Human monocytic leukemia monocyte) whole cell lysates

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 62 kDa
      Observed band size: 52-62 kDa why is the actual band size different from the predicted?



      This antibody recognizes 2 isforms

    • Western blot - Anti-CARD9 antibody [EPR6489] (ab133560)
      Western blot - Anti-CARD9 antibody [EPR6489] (ab133560)
      All lanes : Anti-CARD9 antibody [EPR6489] (ab133560) at 1/1000 dilution (Unpurified)

      Lane 1 : K562 cell lysate
      Lane 2 : U937 cell lysate
      Lane 3 : HL60 cell lysate
      Lane 4 : HepG2 cell lysate
      Lane 5 : THP1 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 62 kDa

    • OI-RD Scanning - Anti-CARD9 antibody [EPR6489] (ab133560)
      OI-RD Scanning - Anti-CARD9 antibody [EPR6489] (ab133560)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-CARD9 antibody [EPR6489] (ab133560)
      Anti-CARD9 antibody [EPR6489] (ab133560)

    References (2)

    Publishing research using ab133560? Please let us know so that we can cite the reference in this datasheet.

    ab133560 has been referenced in 2 publications.

    • Kan B  et al. Cellular metabolism constrains innate immune responses in early human ontogeny. Nat Commun 9:4822 (2018). PubMed: 30446641
    • Drummond RA  et al. CARD9-Dependent Neutrophil Recruitment Protects against Fungal Invasion of the Central Nervous System. PLoS Pathog 11:e1005293 (2015). Flow Cyt ; Human . PubMed: 26679537

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question


    I'm writing to inquire about your antibody: Anti-CARD9 antibody [EPR6489 ] (ab133560).
    The immunogen listed states: Synthetic peptide corresponding to residues in Human CARD9.
    Your other antibodies to CARD9 list the region of the molecule targeted (e.g. ab115578 targets Synthetic peptide: DRENTTGSDNTDTEGS conjugated to KLH, corresponding to amino acids 521-536 of Human CARD9 (NP_434700.2).)
    Can you please specify what amino acid region the ab133560 antibody targets?

    Read More
    Answer

    Thank you for contacting Abcam regarding ab133560.


    The immunogen sequence used to generate this antibody is as follows: XXX (Please contact us for further details.)


    I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

    Read More

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