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    products/primary-antibodies/cardiac-troponin-i-antibody-ep1106y-ab52862.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microfilaments Actin etc Troponin
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RecombinantRabMAb

Recombinant Anti-Cardiac Troponin I antibody [EP1106Y] (ab52862)

  • Datasheet
  • SDS
Reviews (2)Q&A (6)References (15)

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Western blot - Anti-Cardiac Troponin I antibody [EP1106Y] (ab52862)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [EP1106Y] (ab52862)
  • Immunoprecipitation - Anti-Cardiac Troponin I antibody [EP1106Y] (ab52862)
  • Western blot - Anti-Cardiac Troponin I antibody [EP1106Y] (ab52862)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [EP1106Y] (ab52862)
  • Flow Cytometry (Intracellular) - Anti-Cardiac Troponin I antibody [EP1106Y] (ab52862)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [EP1106Y] (ab52862)
  • Anti-Cardiac Troponin I antibody [EP1106Y] (ab52862)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1106Y] to Cardiac Troponin I
  • Suitable for: Flow Cyt (Intra), WB, IP, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-Cardiac Troponin I antibody [EP1106Y]
    See all Cardiac Troponin I primary antibodies
  • Description

    Rabbit monoclonal [EP1106Y] to Cardiac Troponin I
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Cardiac Troponin I aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P19429

  • Positive control

    • WB: Human fetal heart tissue lysate. IHC-P: Human heart and Human cardiac muscle tissues. Flow Cyt (intra): A-673 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1106Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microfilaments
    • Actin etc
    • Troponin
    • Stem Cells
    • Lineage Markers
    • Mesoderm
    • Cardiovascular
    • Heart
    • Contractility
    • Contractile Proteins
    • Troponin
    • Developmental Biology
    • Lineage specification
    • Mesoderm
    • Cardiovascular
    • Cardiovascular Markers
    • Cardiovascular
    • Cardiovascular Markers
    • Cell Markers
    • Cardiomyocytes

Associated products

  • Alternative Versions

    • Anti-Cardiac Troponin I antibody [EP1106Y] - BSA and Azide free (ab182616)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab52862 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/30.
WB
1/100000 - 1/1e+006. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
IP
1/50.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
Flow Cyt (Intra)
1/30.
WB
1/100000 - 1/1e+006. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
IP
1/50.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
  • Involvement in disease

    Defects in TNNI3 are the cause of cardiomyopathy familial hypertrophic type 7 (CMH7) [MIM:613690]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
    Defects in TNNI3 are the cause of cardiomyopathy familial restrictive type 1 (RCM1) [MIM:115210]. RCM1 is an heart muscle disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
    Defects in TNNI3 are the cause of cardiomyopathy dilated type 2A (CMD2A) [MIM:611880]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
    Defects in TNNI3 are the cause of cardiomyopathy dilated type 1FF (CMD1FF) [MIM:613286]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
  • Sequence similarities

    Belongs to the troponin I family.
  • Target information above from: UniProt accession P19429 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 7137 Human
    • Omim: 191044 Human
    • SwissProt: P19429 Human
    • Unigene: 709179 Human
    • Alternative names

      • cardiac muscle antibody
      • Cardiac troponin I antibody
      • cardiomyopathy, dilated 2A (autosomal recessive) antibody
      • Cardiomyopathy, familial hypertrophic, 7, included antibody
      • CMD1FF antibody
      • CMD2A antibody
      • CMH7 antibody
      • cTnI antibody
      • Familial hypertrophic cardiomyopathy 7 antibody
      • MGC116817 antibody
      • RCM1 antibody
      • Tn1 antibody
      • Tni antibody
      • TNN I3 antibody
      • TNNC 1 antibody
      • TNNC1 antibody
      • TNNI3 antibody
      • TNNI3_HUMAN antibody
      • Troponin I antibody
      • Troponin I cardiac antibody
      • Troponin I cardiac muscle antibody
      • Troponin I cardiac muscle isoform antibody
      • Troponin I type 3 cardiac antibody
      • troponin I, cardiac 3 antibody
      • TroponinI antibody
      • Ttroponin I type 3 (cardiac) antibody
      see all

    Images

    • Western blot - Anti-Cardiac Troponin I antibody [EP1106Y] (ab52862)
      Western blot - Anti-Cardiac Troponin I antibody [EP1106Y] (ab52862)
      Anti-Cardiac Troponin I antibody [EP1106Y] (ab52862) at 1/1000000 dilution + fetal heart cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 28 kDa
      Observed band size: 28 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [EP1106Y] (ab52862)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [EP1106Y] (ab52862)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cardiac muscle tissue sections labeling Cardiac Troponin I with Purified ab52862 at 1:300 dilution (1.19 µg/ml). Heat mediated antigen retrieval was performed Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Immunoprecipitation - Anti-Cardiac Troponin I antibody [EP1106Y] (ab52862)
      Immunoprecipitation - Anti-Cardiac Troponin I antibody [EP1106Y] (ab52862)

      ab52862 (purified) at 1:30 dilution (2µg) immunoprecipitating Cardiac Troponin I in Human fetal heart lysate.
      Lane 1 (input): Human fetal heart lysate 10µg
      Lane 2 (+): ab52862 & Human fetal heart lysate
      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52862 in Human fetal heart lysate
      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

    • Western blot - Anti-Cardiac Troponin I antibody [EP1106Y] (ab52862)
      Western blot - Anti-Cardiac Troponin I antibody [EP1106Y] (ab52862)
      Anti-Cardiac Troponin I antibody [EP1106Y] (ab52862) at 1/500000 dilution + Human fetal heart lysates at 15 µg with NFDM/TBST at 5 %

      Secondary
      Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 28 kDa
      Observed band size: 28 kDa



      Diluting buffer: 5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [EP1106Y] (ab52862)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [EP1106Y] (ab52862)

      IHC image of Cardiac Troponin I staining in human heart formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab52862, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times

    • Flow Cytometry (Intracellular) - Anti-Cardiac Troponin I antibody [EP1106Y] (ab52862)
      Flow Cytometry (Intracellular) - Anti-Cardiac Troponin I antibody [EP1106Y] (ab52862)

      Intracellular Flow Cytometry analysis of A-673 (Human muscle Ewing's Sarcoma) cells labeling Cardiac Troponin I (red) with ab52862 at a 1/30 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluorr® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue). 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [EP1106Y] (ab52862)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [EP1106Y] (ab52862)
      ab52862 at 1/250 dilution staining cardiac Troponin I in human cardiac muscle by Immunohistochemistry, Paraffin embedded tissue.
    • Anti-Cardiac Troponin I antibody [EP1106Y] (ab52862)
      Anti-Cardiac Troponin I antibody [EP1106Y] (ab52862)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (15)

    Publishing research using ab52862? Please let us know so that we can cite the reference in this datasheet.

    ab52862 has been referenced in 15 publications.

    • Wu YW  et al. Clinical Feasibility of Biofunctionalized Magnetic Nanoparticles for Detecting Multiple Cardiac Biomarkers in Emergency Chest Pain Patients. Acta Cardiol Sin 36:649-659 (2020). PubMed: 33235422
    • Lewinska A  et al. Remifentanil preconditioning protects against hypoxia-induced senescence and necroptosis in human cardiac myocytes in vitro. Aging (Albany NY) 12:13924-13938 (2020). PubMed: 32584786
    • Todorova D  et al. hESC-derived immune suppressive dendritic cells induce immune tolerance of parental hESC-derived allografts. EBioMedicine 62:103120 (2020). PubMed: 33242828
    • Mura M  et al. Generation of the human induced pluripotent stem cell (hiPSC) line PSMi006-A from a patient affected by an autosomal recessive form of long QT syndrome type 1. Stem Cell Res 42:101658 (2020). PubMed: 31785541
    • Onwuli DO  et al. The inhibitory subunit of cardiac troponin (cTnI) is modified by arginine methylation in the human heart. Int J Cardiol N/A:N/A (2019). PubMed: 30772011
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-8 of 8 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cardiac Troponin I antibody [EP1106Y]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Cardiac)
    Permeabilization
    Yes - 0.05% TritionX100
    Specification
    Cardiac
    Blocking step
    Serum as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Sep 16 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cardiac Troponin I antibody [EP1106Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Cardiomyocytes)
    Specification
    Cardiomyocytes
    Fixative
    Methanol
    Permeabilization
    Yes - 0.2% Triton-x-100
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted May 20 2010

    Question

    Hi
    I recently acquired Epitomics´ Rabbit mAB against cardiac troponin I (EP1106Y - 100 microL - lot GR36789-4 from Abcam). I am using the antibody as a capture Ab on Dynal magnetic beads and for the coupling reaction am interested in knowing the protein concentration of the antibody. Can you supply me with this information or, alternatively a coupling protocol that you may be using for tosyl activated Dynal beads (all sizes).
    Best regards,

    Read More

    Abcam community

    Verified customer

    Asked on Nov 01 2012

    Answer

    Thank you for contacting us.

    The concentration of Rabbit mAB against cardiac troponin I (EP1106Y - 100 microL - lot GR36789-4) is xxxxxxxx mg/ml.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Abcam Scientific Support

    Answered on Nov 01 2012

    Question

    Inquiry: I would like to know it is possible to have the purified Fab fragments of the Anti-cardiac Troponin I antibody (ab52862)

    Read More

    Abcam community

    Verified customer

    Asked on Sep 10 2012

    Answer

    Thank you for contacting us.

    I passed your details on tomy colleagues at Epitomics who will contact you regarding your request.

    Thank you.

    Read More

    Abcam Scientific Support

    Answered on Sep 10 2012

    Question

    Dear,
    Could you provide me with the concentration of one of your products?
    Anti-cardiac Troponin I antibody
    ref : ab52862 lot 877011
    Thank you,

    Read More

    Abcam community

    Verified customer

    Asked on Apr 24 2012

    Answer

    Thank you for your enquiry.

    I can confirm that ab52862 antibody is sold as tissue culture supernatant. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the datasheet. Antibody concentration is usually determined by protein assay, and serum / ascites / tissue culture supernatant will contain a lot of other proteins, which means the antibody quantification would not be accurate.

    I can confirm that for tissue culture supernatant, concentration of antibody is known to very between 1 - 3 mg/ml, however this conc is not the specific IgG present in solution.

    I am sorry we are not able to provide an exact concentration on this occasion, but hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

    Read More

    Abcam Scientific Support

    Answered on Apr 24 2012

    Question

    ab52862 to be tested in Flow Cytometry

    Read More

    Abcam community

    Verified customer

    Asked on Feb 08 2012

    Answer

    DISCOUNT CODE: ***********
    Expiration date: DD MM YYYY

    I am very pleased to hear you would like to accept our offer and test ab52862 in Flow Cytometry. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forFlow Cytometryand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways:
    1) Call to place your order and mention the code to our customer service department;
    2) Include the code in your fax order;
    3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    Answered on Feb 08 2012

    Question

    Hello, Thank´s for your quick answer. The monoclonal isotype control you mentioned (ab99234) is it going to be available in your catalogue later on? It might not be worth ordering that particular control if I won´t be able to order and use it again. In what volume and concentration is the control and what´s the current price?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 21 2011

    Answer

    Thank you for your reply. I am currently in contact with the source of this monoclonal isotype control antibody as due to licensing issues it indeed seems difficult to get it, and I am awaiting a reply. I will forward to you the information as soon as I receive it. In the meantime, following the explanation given in my last mail I could recommend polyclonal isotype controls such as ab27478 or ab37415, depending on the application you would like to perform. Click here (or use the following: https://www.abcam.com/Rabbit-IgG-polyclonal-Isotype-Control-ab27478.html). Click here (or use the following: https://www.abcam.com/Rabbit-IgG-polyclonal-Isotype-Control-ab37415.html). Also, in case you would like to use the isotype control for flow cytometric experiments: Isotype control antibodies are of different clones than the primary antibody of interest and therefore may show varying binding properties compared to the specific antibody of interest. Furthermore, as the conjugation conditions may have been different, the labeling with the fluorochrome can vary which can be a very important issue in flow cytometry. Although isotype controls are frequently used in flow cytometry experiments, it is not the only type of control to determine non-specific binding and positivity for the marker of interest. This control is termed fluorescence minus one or FMO and works like an isotype control without the actual antibody. FMO controls are set up by leaving out one of the antibodies in your staining panel. This control might be also an option for your experiments and you may find more information about this type of control in the latest publications or on flow cytometry websites, such as http://www.isac-net.org or http://www.cyto.purdue.edu/ . If all of this is no option for you and you need the isotype control soon, I would like to recommend checking the Biocompare website which has an excellent antibody search facility that includes many suppliers. The links are: www.biocompare.com or http://www.biocompare.com/ProductCategories/2045/Antibodies.html I am sorry for the confusion and hope this information is nevertheless helpful to you. Please do not hesitate to contact me if you have any further questions in this regard.

    Read More

    Abcam Scientific Support

    Answered on Dec 21 2011

    Question

    Hello,  I´m using Abcam´s rabbit monoclonal cardiac Troponin I antibody (EP1106Y) for my studies. I´m now looking for a proper isotype control and are uncertain whether I can use your polyclonal rabbit IgG isotype control or if I should try to find a monoclonal control instead?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 20 2011

    Answer

    Thank you for your enquiry. If you have a polyclonal rabbit IgG isotype control already at hand, you could use it for this rabbit monoclonal, as amongst other isotypes it may also include the isotype of the monoclonal antibody and therefore resembles some kine of isotype control. Having said this however, I am happy to let you know that we have a rabbit IgG monoclonal isotype control in our catalog (see attached and ab99234: Click here (or use the following: https://www.abcam.com/Rabbit-IgG-monoclonal-Isotype-Control-ab99234.html).) We currently do not have it in stock, but I am more than happy to arrange a delivery for you if you are interested. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Dec 20 2011

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