Recombinant Anti-Cardiac Troponin I antibody [EPR20307] (ab209809)

Product name

Anti-Cardiac Troponin I antibody [EPR20307]
See all Cardiac Troponin I primary antibodies
Description

Rabbit monoclonal [EPR20307] to Cardiac Troponin I
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, IP, IHC-Frmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human heart and myocardium lysates; Mouse and rat heart lysates. IHC-P: Human, mouse and rat cardiac muscle tissues. IP: Human fetal heart lysate. IHC-Fr: Mouse heart tissue, Rat heart tissue
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20307
Isotype

IgG
Research areas

Alternative Versions
- Anti-Cardiac Troponin I antibody [EPR20307] - BSA and Azide free (ab223149)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Related Products
- VeriBlot for IP Detection Reagent (HRP) (ab131366)
- Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab209809 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/2500. Detects a band of approximately 28 kDa (predicted molecular weight: 24 kDa).
IHC-P		1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP		1/30.
IHC-Fr		1/10.

Notes
WB 1/2500. Detects a band of approximately 28 kDa (predicted molecular weight: 24 kDa).
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP 1/30.
IHC-Fr 1/10.

Function

Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Involvement in disease

Defects in TNNI3 are the cause of cardiomyopathy familial hypertrophic type 7 (CMH7) [MIM:613690]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
Defects in TNNI3 are the cause of cardiomyopathy familial restrictive type 1 (RCM1) [MIM:115210]. RCM1 is an heart muscle disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function.
Defects in TNNI3 are the cause of cardiomyopathy dilated type 2A (CMD2A) [MIM:611880]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in TNNI3 are the cause of cardiomyopathy dilated type 1FF (CMD1FF) [MIM:613286]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Sequence similarities

Belongs to the troponin I family.
Target information above from: UniProt accession P19429 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 7137 Human
- Entrez Gene: 21954 Mouse
- Entrez Gene: 29248 Rat
- Omim: 191044 Human
- SwissProt: P19429 Human
- SwissProt: P48787 Mouse
- SwissProt: P23693 Rat
- Unigene: 709179 Human
- Unigene: 27674 Mouse
- Unigene: 64141 Rat
see all
Alternative names
- cardiac muscle antibody
- Cardiac troponin I antibody
- cardiomyopathy, dilated 2A (autosomal recessive) antibody
- Cardiomyopathy, familial hypertrophic, 7, included antibody
- CMD1FF antibody
- CMD2A antibody
- CMH7 antibody
- cTnI antibody
- Familial hypertrophic cardiomyopathy 7 antibody
- MGC116817 antibody
- RCM1 antibody
- Tn1 antibody
- Tni antibody
- TNN I3 antibody
- TNNC 1 antibody
- TNNC1 antibody
- TNNI3 antibody
- TNNI3_HUMAN antibody
- Troponin I antibody
- Troponin I cardiac antibody
- Troponin I cardiac muscle antibody
- Troponin I cardiac muscle isoform antibody
- Troponin I type 3 cardiac antibody
- troponin I, cardiac 3 antibody
- TroponinI antibody
- Ttroponin I type 3 (cardiac) antibody
see all

Western blot - Anti-Cardiac Troponin I antibody [EPR20307] (ab209809)

All lanes : Anti-Cardiac Troponin I antibody [EPR20307] (ab209809) at 1/2500 dilution

Lane 1 : Human heart lysate
Lane 2 : Human myocardium lysate
Lane 3 : Human skeletal muscle lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 24 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?

Exposure time: 3 seconds

Blocking/Dilution buffer: 5% NFDM/TBST.

The expression of cardiac Troponin I is restricted to cardiac muscle.

Negative control:

Human Skeletal muscle

PMID: 1934363

PMID: 8661099
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [EPR20307] (ab209809)

Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling Cardiac Troponin I with ab209809 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on human cardiomyocytes [PMID: 22828728]. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Frozen sections) - Anti-Cardiac Troponin I antibody [EPR20307] (ab209809)

Immunohistochemistry (Frozen) analysis of rat heart tissue section labeling Cardiac Troponin I with purified ab209809 at 1/10 dilution (13.5 µg/ml). Sections were fixed in 0.2% Triton X-100 and permeabilized with DAPI. Antigen retrieval was 4% paraformaldehyde. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/500 dilution (4 µg/ml) dilution. Heat mediated antigen retrieval by using Tris-EDTA buffer (pH9.0) (ab94681) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Frozen sections) - Anti-Cardiac Troponin I antibody [EPR20307] (ab209809)

Immunohistochemistry (Frozen) analysis of mouse heart tissue section labeling Cardiac Troponin I with purified ab209809 at 1/10 dilution (13.5 µg/ml). Sections were fixed in 0.2% Triton X-100 and permeabilized with DAPI. Antigen retrieval was 4% paraformaldehyde. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/500 dilution (4 µg/ml) dilution. Heat mediated antigen retrieval by using Tris-EDTA buffer (pH9.0) (ab94681) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Western blot - Anti-Cardiac Troponin I antibody [EPR20307] (ab209809)

Anti-Cardiac Troponin I antibody [EPR20307] (ab209809) at 1/5000 dilution + Rat heart lysate at 10 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 24 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?

Exposure time: 1 second

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-Cardiac Troponin I antibody [EPR20307] (ab209809)

All lanes : Anti-Cardiac Troponin I antibody [EPR20307] (ab209809) at 1/2500 dilution

Lane 1 : Mouse heart lysate
Lane 2 : C2C12 (Mouse myoblast cell line) whole cell lysate
Lane 3 : Mouse muscle lysate
Lane 4 : Rat muscle lysate
Lane 5 : Human brain lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 24 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?

Exposure time: 1 second

Blocking/Dilution buffer: 5% NFDM/TBST.

The expression of cardiac Troponin I is restricted to cardiac muscle.

Negative control:

C2C12, mouse muscle, rat muscle, human brain

PMID: 1934363

PMID: 8661099
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [EPR20307] (ab209809)

Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling Cardiac Troponin I with ab209809 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Negative control: No staining on human skeletal muscle [PMID: 22828728].

Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [EPR20307] (ab209809)

Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Cardiac Troponin I with ab209809 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on mouse cardiomyocytes [PMID: 22828728]. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [EPR20307] (ab209809)

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Cardiac Troponin I with ab209809 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Negative control: No staining on mouse skeletal muscle [PMID: 22828728].

Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [EPR20307] (ab209809)

Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling Cardiac Troponin I with ab209809 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Positive staining on rat cardiomyocytes [PMID: 22828728]. Counter stained with Hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin I antibody [EPR20307] (ab209809)

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling Cardiac Troponin I with ab209809 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Negative control: No staining on rat skeletal muscle [PMID: 22828728].

Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-Cardiac Troponin I antibody [EPR20307] (ab209809)

Cardiac Troponin I was immunoprecipitated from 0.35 mg of Human fetal heart lysate with ab209809 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab209809 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: Human fetal heart lysate, 10 μg (Input).

Lane 2: ab209809 IP in Human fetal heart lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab209809 in Human fetal heart lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 30 seconds.
Anti-Cardiac Troponin I antibody [EPR20307] (ab209809)

Western blot protocols
Immunoprecipitation protocols
Immunohistochemistry protocols

Click here to view the general protocols

SDS download

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Datasheet download

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Publishing research using ab209809? Please let us know so that we can cite the reference in this datasheet.

ab209809 has been referenced in 4 publications.

Lv L et al. Pretreatment of Nicorandil Protects the Heart from Exhaustive Exercise-Induced Myocardial Injury in Rats. Evid Based Complement Alternat Med 2022:7550872 (2022). PubMed: 35035509
Zhang W et al. Astragaloside IV Alleviates Infarction Induced Cardiomyocyte Injury by Improving Mitochondrial Morphology and Function. Front Cardiovasc Med 9:810541 (2022). PubMed: 35265681
Yu B et al. Circular RNA hsa_circ_105039 promotes cardiomyocyte differentiation by sponging miR‑17 to regulate cyclinD2 expression. Mol Med Rep 24:N/A (2021). PubMed: 34664684
Zheng H et al. Hemin enhances the cardioprotective effects of mesenchymal stem cell-derived exosomes against infarction via amelioration of cardiomyocyte senescence. J Nanobiotechnology 19:332 (2021). PubMed: 34674708

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Related Products

Applications

The Abpromise guarantee

Target

Function

Involvement in disease

Sequence similarities

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (4)

Customer reviews and Q&As