Recombinant Anti-Cardiac Troponin T antibody [EPR20266] (ab209813)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR20266] (ab209813)](https://www.abcam.com/ps/products/209/ab209813/Images/ab209813-270037-anti-cardiac-troponin-t-antibody-epr20266-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR20266] (ab209813)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20266] to Cardiac Troponin T
- Suitable for: IHC-P, WB, Flow Cyt (Intra), ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Cardiac Troponin T antibody [EPR20266]
See all Cardiac Troponin T primary antibodies -
Description
Rabbit monoclonal [EPR20266] to Cardiac Troponin T -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, Flow Cyt (Intra), ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal heart lysate; Mouse and rat heart lysates; IHC-P: Human, mouse and rat cardiac muscle tissues; Flow Cyt (intra): L6 and HL-1 cells. ICC/IF: ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes (ab277612)
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20266 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab209813 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
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| WB |
1/5000. Detects a band of approximately 39 kDa (predicted molecular weight: 36 kDa).
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| Flow Cyt (Intra) |
1/500.
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| ICC/IF | (2) |
Use a concentration of 0.5 - 5 µg/ml.
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| Notes |
|---|
|
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
WB
1/5000. Detects a band of approximately 39 kDa (predicted molecular weight: 36 kDa). |
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Flow Cyt (Intra)
1/500. |
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ICC/IF
Use a concentration of 0.5 - 5 µg/ml. |
Target
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Function
Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. -
Tissue specificity
Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart. -
Involvement in disease
Defects in TNNT2 are the cause of cardiomyopathy familial hypertrophic type 2 (CMH2) [MIM:115195]. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
Defects in TNNT2 are the cause of cardiomyopathy dilated type 1D (CMD1D) [MIM:601494]. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
Defects in TNNT2 are the cause of cardiomyopathy familial restrictive type 3 (RCM3) [MIM:612422]. Restrictive cardiomyopathy is a heart disorder characterized by impaired filling of the ventricles with reduced diastolic volume, in the presence of normal or near normal wall thickness and systolic function. -
Sequence similarities
Belongs to the troponin T family. - Information by UniProt
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Database links
- Entrez Gene: 7139 Human
- Entrez Gene: 21956 Mouse
- Entrez Gene: 24837 Rat
- Omim: 191045 Human
- SwissProt: P45379 Human
- SwissProt: P50752 Mouse
- SwissProt: P50753 Rat
- Unigene: 533613 Human
see all -
Alternative names
- Cardiac muscle troponin T antibody
- Cardiomyopathy dilated 1D (autosomal dominant) antibody
- Cardiomyopathy hypertrophic 2 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR20266] (ab209813)
Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling Cardiac Troponin T with ab209813 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasmic staining on human cardiac muscle is observed [PMID: 9068591].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Cardiac Troponin T antibody [EPR20266] (ab209813)](https://www.abcam.com/ps/products/209/ab209813/Images/ab209813-12-anti-cardiac-troponin-t-immunocytochemistry-antibody-ips-myocytes-human-epr20266.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cardiac Troponin T antibody [EPR20266] (ab209813)Immunofluorescence staining of Cardiac Troponin T using ab209813 in ioSkeletal Myocytes - Human iPSC-Derived Skeletal Myocytes (ab277612), which were differentiated for 10 days post induction.
The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 mins and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab209813 at 1.0 µg/mL and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin, at 1/1000 dilution. Cells were then incubated with ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150120, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown. Gamma is adjusted to 1.5 in all channels.
The antibody ab209813 also gave a positive staining using MeOH fixation (100%, 5 min).
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![Western blot - Anti-Cardiac Troponin T antibody [EPR20266] (ab209813)](https://www.abcam.com/ps/products/209/ab209813/Images/ab209813-270041-anti-cardiac-troponin-t-antibody-epr20266-western-blot.jpg)
Western blot - Anti-Cardiac Troponin T antibody [EPR20266] (ab209813)All lanes : Anti-Cardiac Troponin T antibody [EPR20266] (ab209813) at 1/5000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : Mouse heart lysate
Lane 3 : Human skeletal muscle lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution
Predicted band size: 36 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
Troponin T exists in three tissue-specific subtypes: slow skeletal troponin T1 (ssTnT/TNNT1), fast skeletal troponin T3 (fsTnT/TNNT3) and cardiac troponin T2 (cTnT/TNNT2). The product is specific to cTnT/TNNT2.
Negative control: human skeletal muscle (PMID: 10585344).
The observed molecular weight is consistent with the literature (PMID: 10451242).
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![Western blot - Anti-Cardiac Troponin T antibody [EPR20266] (ab209813)](https://www.abcam.com/ps/products/209/ab209813/Images/ab209813-270038-anti-cardiac-troponin-t-antibody-epr20266-western-blot.jpg)
Western blot - Anti-Cardiac Troponin T antibody [EPR20266] (ab209813)Anti-Cardiac Troponin T antibody [EPR20266] (ab209813) at 1/5000 dilution + Rat heart lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 36 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
The observed molecular weight is consistent with the literature (PMID: 10451242).
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR20266] (ab209813)](https://www.abcam.com/ps/products/209/ab209813/Images/ab209813-270036-anti-cardiac-troponin-t-antibody-epr20266-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR20266] (ab209813)Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling Cardiac Troponin T with ab209813 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Negative control: No staining on human skeletal muscle [PMID: 11375287].
Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR20266] (ab209813)](https://www.abcam.com/ps/products/209/ab209813/Images/ab209813-270035-anti-cardiac-troponin-t-antibody-epr20266-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR20266] (ab209813)Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Cardiac Troponin T with ab209813 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasmic staining on mouse cardiac muscle is observed [PMID: 9068591].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR20266] (ab209813)](https://www.abcam.com/ps/products/209/ab209813/Images/ab209813-270034-anti-cardiac-troponin-t-antibody-epr20266-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR20266] (ab209813)Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Cardiac Troponin T with ab209813 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Negative control: No staining on mouse skeletal muscle [PMID: 11375287].
Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR20266] (ab209813)](https://www.abcam.com/ps/products/209/ab209813/Images/ab209813-270033-anti-cardiac-troponin-t-antibody-epr20266-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR20266] (ab209813)Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling Cardiac Troponin T with ab209813 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasmic staining on rat cardiac muscle is observed [PMID: 9068591].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR20266] (ab209813)](https://www.abcam.com/ps/products/209/ab209813/Images/ab209813-270032-anti-cardiac-troponin-t-antibody-epr20266-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cardiac Troponin T antibody [EPR20266] (ab209813)Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling Cardiac Troponin T with ab209813 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Negative control: No staining on rat skeletal muscle [PMID: 11375287].
Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
![Flow Cytometry (Intracellular) - Anti-Cardiac Troponin T antibody [EPR20266] (ab209813)](https://www.abcam.com/ps/products/209/ab209813/Images/ab209813-10-anti-cardiac-troponin-t-antibody-epr20266-flow-cytometry-l6.jpg)
Flow Cytometry (Intracellular) - Anti-Cardiac Troponin T antibody [EPR20266] (ab209813)Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized L6 (Rat skeletal muscle myoblast) cells labelling Cardiac Troponin T with ab209813 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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![Flow Cytometry (Intracellular) - Anti-Cardiac Troponin T antibody [EPR20266] (ab209813)](https://www.abcam.com/ps/products/209/ab209813/Images/ab209813-9-anti-cardiac-troponin-t-antibody-epr20266-flow-cytometry-hl-1.jpg)
Flow Cytometry (Intracellular) - Anti-Cardiac Troponin T antibody [EPR20266] (ab209813)Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HL-1 (Mouse atrial muscle cell) cells labelling Cardiac Troponin T with ab209813 at 1/500 dilution (0.1µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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![Anti-Cardiac Troponin T antibody [EPR20266] (ab209813)](https://www.abcam.com/ps/products/209/ab209813/Images/ab209813-11-benefits-of-recombinant-antibodies.png)
Anti-Cardiac Troponin T antibody [EPR20266] (ab209813)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (3)
ab209813 has been referenced in 3 publications.
- Xing W et al. MiR-33a-5p targets NOMO1 to modulate human cardiomyocyte progenitor cells proliferation and differentiation and apoptosis. J Recept Signal Transduct Res 41:476-487 (2021). PubMed: 33054489
- Jeremic JN et al. Garlic Derived Diallyl Trisulfide in Experimental Metabolic Syndrome: Metabolic Effects and Cardioprotective Role. Int J Mol Sci 21:N/A (2020). PubMed: 33265949
- Yi Q et al. Islet-1 induces the differentiation of mesenchymal stem cells into cardiomyocyte-like cells through the regulation of Gcn5 and DNMT-1. Mol Med Rep 15:2511-2520 (2017). PubMed: 28447752