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    products/primary-antibodies/carm1-antibody-epr23678-113-ab243638.pdf

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Cell Biology Cell Cycle Cell Cycle Inhibitors p53
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-CARM1 antibody [EPR23678-113] (ab243638)

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Western blot - Anti-CARM1 antibody [EPR23678-113] (ab243638)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARM1 antibody [EPR23678-113] (ab243638)
  • Flow Cytometry (Intracellular) - Anti-CARM1 antibody [EPR23678-113] (ab243638)
  • Western blot - Anti-CARM1 antibody [EPR23678-113] (ab243638)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARM1 antibody [EPR23678-113] (ab243638)
  • Flow Cytometry (Intracellular) - Anti-CARM1 antibody [EPR23678-113] (ab243638)
  • Western blot - Anti-CARM1 antibody [EPR23678-113] (ab243638)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARM1 antibody [EPR23678-113] (ab243638)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARM1 antibody [EPR23678-113] (ab243638)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23678-113] to CARM1
  • Suitable for: WB, IHC-P, Flow Cyt (Intra)
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Primary
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Anti-LRIG3 antibody (ab197989)
Knockout
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Human CARM1 knockout HEK-293T cell line (ab266557)
Protein
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Recombinant Human VAMP2 protein (ab49001)

View more associated products

Overview

  • Product name

    Anti-CARM1 antibody [EPR23678-113]
    See all CARM1 primary antibodies
  • Description

    Rabbit monoclonal [EPR23678-113] to CARM1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt (Intra)more details
    Unsuitable for: ChIP,ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Wild-type HEK-293T; HeLa; HCT116; NIH/3T3 whole cell lysates; Mouse liver and lung tissue lysates; Rat liver tissue lysate. IHC-P: Human colon and colon carcinoma; Mouse liver; Rat colon. Flow Cyt (intra): Wild-type HEK-293T, HeLa cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR23678-113
  • Isotype

    IgG1
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Cycle Inhibitors
    • p53
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Methylation
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • Nuclear Hormone Receptors
    • Co-activators/co-repressors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Cell Cycle Inhibitors
    • p53
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Co-factors
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Methylation
    • Arginine Methylation
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • p53 pathway
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Cofactors, Vitamins / minerals
    • Co-factors

Associated products

  • Alternative Versions

    • Anti-CARM1 antibody [EPR23678-113] - BSA and Azide free (ab279344)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • KO cell lines

    • Human CARM1 knockout HEK-293T cell line (ab266557)
  • KO cell lysates

    • Human CARM1 knockout HEK-293T cell lysate (ab257871)
  • Related Products

    • Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776)
    • Anti-GAPDH antibody [6C5] - Loading Control (ab8245)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab243638 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Predicted molecular weight: 65 kDa.
IHC-P
1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt (Intra)
1/500.
Notes
WB
1/1000. Predicted molecular weight: 65 kDa.
IHC-P
1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cyt (Intra)
1/500.
Application notes
Is unsuitable for ChIP,ICC/IF or IP.

Target

  • Function

    Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activate transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg-2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs.
  • Tissue specificity

    Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia.
  • Sequence similarities

    Belongs to the protein arginine N-methyltransferase family.
  • Post-translational
    modifications

    Auto-methylated on Arg-550. Methylation enhances transcription coactivator activity. Methylation is required for its role in the regulation of pre-mRNA alternative splicing.
    Phosphorylation at Ser-216 interferes with S-adenosyl-L-methionine binding and strongly reduces methyltransferase activity (By similarity). Phosphorylation at Ser-216 is strongly increased during mitosis, and decreases rapidly to a very low, basal level after entry into the G1 phase of the cell cycle. Phosphorylation at Ser-216 may promote location in the cytosol.
  • Cellular localization

    Nucleus. Cytoplasm. Mainly nuclear during the G1, S and G2 phases of the cell cycle. Cytoplasmic during mitosis, after breakup of the nuclear membrane.
  • Target information above from: UniProt accession Q86X55 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10498 Human
    • Entrez Gene: 59035 Mouse
    • Entrez Gene: 363026 Rat
    • Omim: 603934 Human
    • SwissProt: Q86X55 Human
    • SwissProt: Q9WVG6 Mouse
    • SwissProt: Q4AE70 Rat
    • Unigene: 323213 Human
    • Unigene: 178115 Mouse
    see all
  • Alternative names

    • carm1 antibody
    • CARM1_HUMAN antibody
    • Coactivator associated arginine methyltransferase 1 antibody
    • Coactivator-associated arginine methyltransferase 1 antibody
    • Histone arginine methyltransferase CARM 1 antibody
    • Histone arginine methyltransferase CARM1 antibody
    • Histone-arginine methyltransferase CARM1 antibody
    • PRMT 4 antibody
    • PRMT4 antibody
    • Protein arginine methyltransferase antibody
    • Protein arginine N methyltransferase 4 antibody
    • Protein arginine N-methyltransferase 4 antibody
    see all

Images

  • Western blot - Anti-CARM1 antibody [EPR23678-113] (ab243638)
    Western blot - Anti-CARM1 antibody [EPR23678-113] (ab243638)
    All lanes : Anti-CARM1 antibody [EPR23678-113] (ab243638) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T (human embryonic kidney epithelial cell), whole cell lysate
    Lane 2 : CARM1 knockout HEK-293T (human embryonic kidney epithelial cell), whole cell lysate
    Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 4 : HCT116 (human colorectal carcinoma epithelial cell), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) at 1/10000 dilution

    Predicted band size: 65 kDa
    Observed band size: 65 kDa



    Blocking and diluting buffer and concentration: Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS.

    Lanes 1 - 4: Merged signal (red and green). Green - ab243638 observed at 65 kDa. Red - loading control ab8245 (Mouse monoclonal [6C5] to GAPDH) observed at 36 kDa.

    ab243638 was shown to react with CARM1 in wild-type HEK-293T cells in western blot with loss of signal observed in CARM1 knockout cell line ab266557 (CARM1 knockout cell lysate ab257871). Wild-type and CARM1 knockout HEK-293T cell lysates were subjected to SDS-PAGE. 

    ab243638 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at 4°C overnight at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

    Boiled samples are used in this blot.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARM1 antibody [EPR23678-113] (ab243638)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARM1 antibody [EPR23678-113] (ab243638)

    Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CARM1 with ab243638 at 1/200 dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human colon (PMID: 20462455). The section was incubated with ab243638 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Flow Cytometry (Intracellular) - Anti-CARM1 antibody [EPR23678-113] (ab243638)
    Flow Cytometry (Intracellular) - Anti-CARM1 antibody [EPR23678-113] (ab243638)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Wild-type HEK-293T (human embryonic kidney epithelial cell, Right panel)/ CARM1 knockout HEK-293T cells (Left panel) cells labeling CARM1 with ab243638 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    Positive staining on HEK-293T cells (ab255449), while no staining on CARM1 knockout HEK-293T cells (ab266557).

  • Western blot - Anti-CARM1 antibody [EPR23678-113] (ab243638)
    Western blot - Anti-CARM1 antibody [EPR23678-113] (ab243638)
    All lanes : Anti-CARM1 antibody [EPR23678-113] (ab243638) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 2 : HCT116 (human colorectal carcinoma epithelial cell), whole cell lysate
    Lane 3 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 65 kDa
    Observed band size: 65 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Samples are non-boiled as boiling may cause protein aggregates

    Exposure time: 10 seconds

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARM1 antibody [EPR23678-113] (ab243638)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARM1 antibody [EPR23678-113] (ab243638)

    Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labeling CARM1 with ab243638 at 1/200 dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human colon carcinoma (PMID: 20462455). The section was incubated with ab243638 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Flow Cytometry (Intracellular) - Anti-CARM1 antibody [EPR23678-113] (ab243638)
    Flow Cytometry (Intracellular) - Anti-CARM1 antibody [EPR23678-113] (ab243638)

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling CARM1 with ab243638 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-CARM1 antibody [EPR23678-113] (ab243638)
    Western blot - Anti-CARM1 antibody [EPR23678-113] (ab243638)
    All lanes : Anti-CARM1 antibody [EPR23678-113] (ab243638) at 1/1000 dilution

    Lane 1 : Mouse liver tissue lysate
    Lane 2 : Mouse lung tissue lysate
    Lane 3 : Rat liver tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 65 kDa
    Observed band size: 65 kDa



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Samples are non-boiled as boiling may cause protein aggregates

    Exposure times:

    Lane 1-2: 15 seconds

    Lane 3: 92 seconds

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARM1 antibody [EPR23678-113] (ab243638)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARM1 antibody [EPR23678-113] (ab243638)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling CARM1 with ab243638 at 1/200 dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat colon. The section was incubated with ab243638 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARM1 antibody [EPR23678-113] (ab243638)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARM1 antibody [EPR23678-113] (ab243638)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling CARM1 with ab243638 at 1/200 dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse liver. The section was incubated with ab243638 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (0)

Publishing research using ab243638? Please let us know so that we can cite the reference in this datasheet.

ab243638 has not yet been referenced specifically in any publications.

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