Recombinant Anti-CARM1 antibody [EPR23678-113] (ab243638)
![Western blot - Anti-CARM1 antibody [EPR23678-113] (ab243638)](https://www.abcam.com/ps/products/243/ab243638/Images/ab243638-451607-anti-carm1-antibody-epr23678113-western-blot-human.jpg "Western blot - Anti-CARM1 antibody [EPR23678-113] (ab243638)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23678-113] to CARM1
- Suitable for: WB, IHC-P, Flow Cyt (Intra)
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-CARM1 antibody [EPR23678-113]
See all CARM1 primary antibodies -
Description
Rabbit monoclonal [EPR23678-113] to CARM1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cyt (Intra)more details
Unsuitable for: ChIP,ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Wild-type HEK-293T; HeLa; HCT116; NIH/3T3 whole cell lysates; Mouse liver and lung tissue lysates; Rat liver tissue lysate. IHC-P: Human colon and colon carcinoma; Mouse liver; Rat colon. Flow Cyt (intra): Wild-type HEK-293T, HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23678-113 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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KO cell lines
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KO cell lysates
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab243638 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 65 kDa.
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| IHC-P |
1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| Flow Cyt (Intra) |
1/500.
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| Notes |
|---|
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WB
1/1000. Predicted molecular weight: 65 kDa. |
|
IHC-P
1/200. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
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Flow Cyt (Intra)
1/500. |
Target
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Function
Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activate transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg-2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs. -
Tissue specificity
Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia. -
Sequence similarities
Belongs to the protein arginine N-methyltransferase family. -
Post-translational
modificationsAuto-methylated on Arg-550. Methylation enhances transcription coactivator activity. Methylation is required for its role in the regulation of pre-mRNA alternative splicing.
Phosphorylation at Ser-216 interferes with S-adenosyl-L-methionine binding and strongly reduces methyltransferase activity (By similarity). Phosphorylation at Ser-216 is strongly increased during mitosis, and decreases rapidly to a very low, basal level after entry into the G1 phase of the cell cycle. Phosphorylation at Ser-216 may promote location in the cytosol. -
Cellular localization
Nucleus. Cytoplasm. Mainly nuclear during the G1, S and G2 phases of the cell cycle. Cytoplasmic during mitosis, after breakup of the nuclear membrane. - Information by UniProt
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Database links
- Entrez Gene: 10498 Human
- Entrez Gene: 59035 Mouse
- Entrez Gene: 363026 Rat
- Omim: 603934 Human
- SwissProt: Q86X55 Human
- SwissProt: Q9WVG6 Mouse
- SwissProt: Q4AE70 Rat
- Unigene: 323213 Human
see all -
Alternative names
- carm1 antibody
- CARM1_HUMAN antibody
- Coactivator associated arginine methyltransferase 1 antibody
see all
Images
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Western blot - Anti-CARM1 antibody [EPR23678-113] (ab243638)All lanes : Anti-CARM1 antibody [EPR23678-113] (ab243638) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T (human embryonic kidney epithelial cell), whole cell lysate
Lane 2 : CARM1 knockout HEK-293T (human embryonic kidney epithelial cell), whole cell lysate
Lane 3 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 4 : HCT116 (human colorectal carcinoma epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) at 1/10000 dilution
Predicted band size: 65 kDa
Observed band size: 65 kDaBlocking and diluting buffer and concentration: Intercept® (TBS) Blocking Buffer diluted with an equal volume of 0.1% TBS.
Lanes 1 - 4: Merged signal (red and green). Green - ab243638 observed at 65 kDa. Red - loading control ab8245 (Mouse monoclonal [6C5] to GAPDH) observed at 36 kDa.
ab243638 was shown to react with CARM1 in wild-type HEK-293T cells in western blot with loss of signal observed in CARM1 knockout cell line ab266557 (CARM1 knockout cell lysate ab257871). Wild-type and CARM1 knockout HEK-293T cell lysates were subjected to SDS-PAGE.
ab243638 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at 4°C overnight at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
Boiled samples are used in this blot.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARM1 antibody [EPR23678-113] (ab243638)](https://www.abcam.com/ps/products/243/ab243638/Images/ab243638-1-anti-carm1-antibody-epr23678113-immunohistochemistry-human-colon.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARM1 antibody [EPR23678-113] (ab243638)Immunohistochemical analysis of paraffin-embedded human colon tissue labeling CARM1 with ab243638 at 1/200 dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human colon (PMID: 20462455). The section was incubated with ab243638 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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![Flow Cytometry (Intracellular) - Anti-CARM1 antibody [EPR23678-113] (ab243638)](https://www.abcam.com/ps/products/243/ab243638/Images/ab243638-5-anti-carm1-antibody-epr23678113-flow-cytometry-wildtype-hek293t.jpg)
Flow Cytometry (Intracellular) - Anti-CARM1 antibody [EPR23678-113] (ab243638)Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Wild-type HEK-293T (human embryonic kidney epithelial cell, Right panel)/ CARM1 knockout HEK-293T cells (Left panel) cells labeling CARM1 with ab243638 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Positive staining on HEK-293T cells (ab255449), while no staining on CARM1 knockout HEK-293T cells (ab266557).
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![Western blot - Anti-CARM1 antibody [EPR23678-113] (ab243638)](https://www.abcam.com/ps/products/243/ab243638/Images/ab243638-451608-anti-carm1-antibody-epr23678113-western-blot-adenocarcinoma.jpg)
Western blot - Anti-CARM1 antibody [EPR23678-113] (ab243638)All lanes : Anti-CARM1 antibody [EPR23678-113] (ab243638) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : HCT116 (human colorectal carcinoma epithelial cell), whole cell lysate
Lane 3 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 65 kDa
Observed band size: 65 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Samples are non-boiled as boiling may cause protein aggregates
Exposure time: 10 seconds
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARM1 antibody [EPR23678-113] (ab243638)](https://www.abcam.com/ps/products/243/ab243638/Images/ab243638-2-anti-carm1-antibody-epr23678113-immunohistochemistry-human-colon-carcinoma.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARM1 antibody [EPR23678-113] (ab243638)Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labeling CARM1 with ab243638 at 1/200 dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human colon carcinoma (PMID: 20462455). The section was incubated with ab243638 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Flow Cytometry (Intracellular) - Anti-CARM1 antibody [EPR23678-113] (ab243638)](https://www.abcam.com/ps/products/243/ab243638/Images/ab243638-6-anti-carm1-antibody-epr23678113-flow-cytometry-hela.jpg)
Flow Cytometry (Intracellular) - Anti-CARM1 antibody [EPR23678-113] (ab243638)Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling CARM1 with ab243638 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
-
![Western blot - Anti-CARM1 antibody [EPR23678-113] (ab243638)](https://www.abcam.com/ps/products/243/ab243638/Images/ab243638-451609-anti-carm1-antibody-epr23678113-western-blot-lysate.jpg)
Western blot - Anti-CARM1 antibody [EPR23678-113] (ab243638)All lanes : Anti-CARM1 antibody [EPR23678-113] (ab243638) at 1/1000 dilution
Lane 1 : Mouse liver tissue lysate
Lane 2 : Mouse lung tissue lysate
Lane 3 : Rat liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 65 kDa
Observed band size: 65 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Samples are non-boiled as boiling may cause protein aggregates
Exposure times:
Lane 1-2: 15 seconds
Lane 3: 92 seconds
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARM1 antibody [EPR23678-113] (ab243638)](https://www.abcam.com/ps/products/243/ab243638/Images/ab243638-4-anti-carm1-antibody-epr23678113-immunohistochemistry-rat-colon.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARM1 antibody [EPR23678-113] (ab243638)Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling CARM1 with ab243638 at 1/200 dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on rat colon. The section was incubated with ab243638 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARM1 antibody [EPR23678-113] (ab243638)](https://www.abcam.com/ps/products/243/ab243638/Images/ab243638-3-anti-carm1-antibody-epr23678113-immunohistochemistry-mouse-liver.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CARM1 antibody [EPR23678-113] (ab243638)Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling CARM1 with ab243638 at 1/200 dilution followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on mouse liver. The section was incubated with ab243638 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab243638 has not yet been referenced specifically in any publications.