Anti-Caspase-9 antibody (ab2013)

Reviews (2)Q&A (5)References (36)
Western blot - Anti-Caspase-9 antibody (ab2013)
  • Western blot - Anti-Caspase-9 antibody (ab2013)
  • Western blot - Anti-Caspase-9 antibody (ab2013)
  • Western blot - Anti-Caspase-9 antibody (ab2013)
  • Western blot - Anti-Caspase-9 antibody (ab2013)

Key features and details

  • Rabbit polyclonal to Caspase-9
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-Caspase-9 antibody
    See all Caspase-9 primary antibodies

  • Description

    Rabbit polyclonal to Caspase-9

  • Host species

    Rabbit

  • Specificity

    No cross reactivity with other members of the caspase family. This antibody does not recognise the cleaved form of Caspase-9.

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to Human Caspase-9 aa 41-56.
    Sequence:

    EDIQRAGSGSRRDQAR


    Database link: P55211
    (Peptide available as ab7885)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
1/1000 - 1/2000. Predicted molecular weight: 46 kDa.
Notes
WB
1/1000 - 1/2000. Predicted molecular weight: 46 kDa.

Target

  • Function

    Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
    Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.

  • Tissue specificity

    Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.

  • Sequence similarities

    Belongs to the peptidase C14A family.
    Contains 1 CARD domain.

  • Developmental stage

    Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.

  • Post-translational
    modifications

    Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
  • Information by UniProt

  • Database links

    • Alternative names

      • APAF-3 antibody
      • APAF3 antibody
      • Apoptosis related cysteine peptidase antibody
      • Apoptotic protease Mch-6 antibody
      • Apoptotic protease-activating factor 3 antibody
      • CASP-9 antibody
      • CASP9 antibody
      • CASP9_HUMAN antibody
      • Caspase 9 apoptosis related cysteine peptidase antibody
      • Caspase 9 Dominant Negative antibody
      • Caspase 9c antibody
      • Caspase-9 antibody
      • Caspase-9 subunit p10 antibody
      • ICE LAP6 antibody
      • ICE like apoptotic protease 6 antibody
      • ICE-LAP6 antibody
      • ICE-like apoptotic protease 6 antibody
      • MCH6 antibody
      • PPP1R56 antibody
      • protein phosphatase 1, regulatory subunit 56 antibody
      • RNCASP9 antibody
      see all

    Images

    • Western blot - Anti-Caspase-9 antibody (ab2013)
      Western blot - Anti-Caspase-9 antibody (ab2013)
      All lanes : Anti-Caspase-9 antibody (ab2013) at 1 µg/ml

      Lane 1 : Daudi cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : K562 cell lysate

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

      Predicted band size: 46 kDa

    • Western blot - Anti-Caspase-9 antibody (ab2013)
      Western blot - Anti-Caspase-9 antibody (ab2013)
      Lane 1 : Anti-Caspase-9 antibody (ab2013) at 1 µg/ml
      Lane 2 : Anti-Caspase-9 antibody (ab2013) at 2 µg/ml
      Lane 3 : Anti-Caspase-9 antibody (ab2013) at 4 µg/ml

      All lanes : Human heart lysate

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

      Predicted band size: 46 kDa

    • Western blot - Anti-Caspase-9 antibody (ab2013)
      Western blot - Anti-Caspase-9 antibody (ab2013)
      Lane 1 : Anti-Caspase-9 antibody (ab2013) at 0.5 µg/ml
      Lane 2 : Anti-Caspase-9 antibody (ab2013) at 1 µg/ml
      Lane 3 : Anti-Caspase-9 antibody (ab2013) at 2 µg/ml

      All lanes : Mouse spleen lysate

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

      Predicted band size: 46 kDa

    • Western blot - Anti-Caspase-9 antibody (ab2013)
      Western blot - Anti-Caspase-9 antibody (ab2013)
      All lanes : Anti-Caspase-9 antibody (ab2013) at 0.5 µg

      All lanes : Rat spleen lysate

      Lysates/proteins at 15 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

      Predicted band size: 46 kDa

    • Western blot - Anti-Caspase-9 antibody (ab2013)
      Western blot - Anti-Caspase-9 antibody (ab2013)
      Anti-Caspase-9 antibody (ab2013) at 1/1000 dilution + HeLa whole cell lysate

      Predicted band size: 46 kDa
      Observed band size: 46 kDa

    References (36)

    Publishing research using ab2013? Please let us know so that we can cite the reference in this datasheet.

    ab2013 has been referenced in 36 publications.

    • Meng L  et al. Costunolide attenuates oxygen-glucose deprivation/reperfusion-induced mitochondrial-mediated apoptosis in PC12 cells. Mol Med Rep 23:N/A (2021). PubMed: 33786628
    • Wang Y  et al. Discovery of LAMP-2A as potential biomarkers for glioblastoma development by modulating apoptosis through N-CoR degradation. Cell Commun Signal 19:40 (2021). PubMed: 33761934
    • Liu H  et al. Effects of repetitive magnetic stimulation on motor function and GAP43 and 5-HT expression in rats with spinal cord injury. J Int Med Res 48:300060520970765 (2020). PubMed: 33356694
    • Sun S  et al. CCN5 inhibits proliferation and promotes apoptosis of oral squamous cell carcinoma cells. Cell Biol Int 44:998-1008 (2020). PubMed: 31889370
    • Wang L  et al. MiR-23b functions as an oncogenic miRNA by downregulating Mcl-1S in lung cancer cell line A549. J Biochem Mol Toxicol 34:e22494 (2020). PubMed: 32281274
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-7 of 7 Abreviews or Q&A

    Western blot abreview for Anti-Caspase-9 antibody

     Average
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Pig Cell lysate - other (Lion, muscle tissue)
    Gel Running Conditions
    Reduced Denaturing (8-16%)
    Loading amount
    150 µg
    Specification
    Lion, muscle tissue
    Blocking step
    Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Mayra González

    Verified customer

    Submitted Jul 14 2016

    Western blot abreview for Anti-Caspase 9 antibody

     Good
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (stomach cancer)
    Loading amount
    25 µg
    Specification
    stomach cancer
    Treatment
    10uM WIN 55,212-2 for 24hrs
    Gel Running Conditions
    Reduced Denaturing (10% SDS-PAGE)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 27 2013

    Question

    DESCRIPTION OF THE PROBLEM Does the casp9 antibody ab2013 detect your casp9 protein ab52203? If not can you recommend an antibody that will detect it so I can order the protein and ab together next week? I would like to perform a binding / inhibition experiment using these products if they are compatible.

    Read More
    Answer

    Thank you for your enquiry. Antibody ab2013 will recognize the recombinant CASP9 protein ab52203. Based on the units of ab52203 per vial received, you will receive about 62ug recombinant caspase 9 protein in a vial of ab52203. I hope this is helpful. Please contact me again if you have any further questions.

    Read More

    Question

    Customer has found that ab2013 does not recognise the cleaved form of the protein following the supplier providing the information that it does. Offered a replacement vial of ab25758 as a replacement in return for an Abreview.

    Read More
    Answer

    Thank you for your enquiry. Further to our telephone conversation this morning I have updated our datasheet regarding the specificity of this antibody for the uncleaved form of Caspase 9. I look forward to reading your Abreview. I have arranged for a free of charge replacement vial of ab25758 to be dispatched immediately (order number: 135138). I hope that this does not affect your choice of Abcam in the future.

    Read More

    Question

    Antibody MUST recognise the cleaved (and precursor) of Caspase 8 and 9 in WB

    Read More
    Answer

    Thank you for your email. I have performed a search of our catalogue and I can tell you that ab28142 and ab2013 are your best antibodies for the detection of cleaved and uncleaved caspase 8 and 9 by western blotting. The notes of each antibody are as follows: ab28142 detects ~55 and 57 kDa proteins, corresponding to the apparent molecular masses of the proform of caspase-8 alpha and beta on SDS-PAGE immunoblots. In apoptotic cells, this antibody also detects the processed forms (42/44 kDa, 31, 26, 18 and 14kDa). ab2013 detects both the activated (35 or 37kDa cleaved fragment depending on activator) and non-activated caspase-9 (45kDa). I would recommend that you have a look at the antibody's respective datasheets by clicking on the links below. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Question

    has anybody tried caspase 9 for immunohistochemistry? is there any information on this?

    Read More
    Answer

    No, to our knowledge, this anitbody has only been tested in WB.

    Read More

    Question

    DOES THIS AB RECOGNIZE MOUSE CASPASE 9? CAN THIS AB DETECT ONLY THE 46 KDA PROTEIN? DOES IT CROSS REACT WITH THE ACTIVE CASPASE 9 (36 KDA)?

    Read More
    Answer

    This antibody was made with a peptide located at amino acids 41-56 of human caspase-9. The peptide is located in the cleaved and activated fragment (35 or 37 kDa). Therefore, it should recognize the precursor and activated form although we have not tested the activated form. Our Western Blot tests show a strong, single band at 46kDa. It is human, mouse, and rat reactive.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com