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Anti-CaSR antibody [5C10, ADD] (ab19347)

Reviews (5)Q&A (11)References (54)
Immunohistochemistry (Frozen sections) - Anti-CaSR antibody [5C10, ADD] (ab19347)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaSR antibody [5C10, ADD] (ab19347)
  • Flow Cytometry - Anti-CaSR antibody [5C10, ADD] (ab19347)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaSR antibody [5C10, ADD] (ab19347)
  • Immunohistochemistry (Frozen sections) - Anti-CaSR antibody [5C10, ADD] (ab19347)

Key features and details

  • Mouse monoclonal [5C10, ADD] to CaSR
  • Suitable for: IHC-P, Flow Cyt, IHC-Fr
  • Reacts with: Mouse, Human
  • Isotype: IgG2a

Overview

  • Product name

    Anti-CaSR antibody [5C10, ADD]
    See all CaSR primary antibodies

  • Description

    Mouse monoclonal [5C10, ADD] to CaSR

  • Host species

    Mouse

  • Tested applications

    Suitable for: IHC-P, Flow Cyt, IHC-Frmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide within Human CaSR aa 200-300. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab19347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (1)
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
Use 1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. 
IHC-Fr (3)
Use a concentration of 2 µg/ml.
Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
Use 1µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. 
IHC-Fr
Use a concentration of 2 µg/ml.

Target

  • Function

    Senses changes in the extracellular concentration of calcium ions. The activity of this receptor is mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system.

  • Tissue specificity

    Expressed in the temporal lobe, frontal lobe, parietal lobe, hippocampus, and cerebellum. Also found in kidney, lung, liver, heart, skeletal muscle, placenta.

  • Involvement in disease

    Defects in CASR are the cause of familial hypocalciuric hypercalcemia type 1 (FHH) [MIM:145980]. FHH is characterized by altered calcium homeostasis. Affected individuals exhibit mild or modest hypercalcemia, relative hypocalciuria, and inappropriately normal PTH levels.
    Defects in CASR are the cause of neonatal severe primary hyperparathyroidism (NSHPT) [MIM:239200]. NSHPT is a rare autosomal recessive life-threatening disorder characterized by very high serum calcium concentrations, skeletal demineralization, and parathyroid hyperplasia. In some instances NSHPT has been demonstrated to be the homozygous form of FHH.
    Defects in CASR are a cause of familial isolated hypoparathyroidism (FIH) [MIM:146200]; also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. An autosomal recessive form of FIH also exists.
    Defects in CASR are the cause of idiopathic generalized epilepsy type 8 (IGE8) [MIM:612899]; also known as EIG8. A disorder characterized by recurring generalized seizures in the absence of detectable brain lesions and/or metabolic abnormalities. Seizure types are variable, but include myoclonic seizures, absence seizures, febrile seizures, complex partial seizures, and generalized tonic-clonic seizures.
    Note=Homozygous defects in CASR can be a cause of primary hyperparathyroidism in adulthood. Patients suffer from osteoporosis and renal calculi, have marked hypercalcemia and increased serum PTH concentrations.

  • Sequence similarities

    Belongs to the G-protein coupled receptor 3 family.

  • Post-translational
    modifications

    N-glycosylated.
    Ubiquitinated by RNF19A; which induces proteasomal degradation.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • Ca sensing receptor antibody
      • Ca2+ sensing receptor 1 antibody
      • Ca2+ sensing receptor antibody
      • Calcium sensing receptor antibody
      • CAR antibody
      • CaSR antibody
      • CASR_HUMAN antibody
      • EIG8 antibody
      • Extracellular calcium sensing receptor antibody
      • Extracellular calcium sensing receptor [Precursor] antibody
      • Extracellular calcium-sensing receptor antibody
      • FHH antibody
      • FIH antibody
      • GPRC2A antibody
      • HHC antibody
      • HHC1 antibody
      • Hypocalciuric hypercalcemia 1 antibody
      • Hypocalciuric hypercalcemia 1 severe neonatal hyperparathyroidism antibody
      • MGC138441 antibody
      • NSHPT antibody
      • Parathyroid Ca(2+) sensing receptor 1 antibody
      • Parathyroid Cell calcium sensing receptor antibody
      • Parathyroid Cell calcium-sensing receptor antibody
      • PCAR 1 antibody
      • PCaR1 antibody
      see all

    Images

    • Immunohistochemistry (Frozen sections) - Anti-CaSR antibody [5C10, ADD] (ab19347)
      Immunohistochemistry (Frozen sections) - Anti-CaSR antibody [5C10, ADD] (ab19347)

      Immunohistochemical analysis of 4% formalin fixed frozen mouse stomach tissue labeling CaSR with ab19347 at 1/100 dilution overnight at 4°C, followed by fluorophore-conjugated goat anti-mouse IgG secondary antibody for 2 hours at RT.

      Fresh stomach tissue was fixed in 4% formalin for 1 hour and then incubated overnight at  in 25% sucrose before embedding in tissue freezing medium. Antigen retrieval was carried out on 8µm cryo-sections by incubating in sodium citrate buffer for 45 minutes at 4°C, immersing in sodium citrate buffer for 10 minutes at 100°C before washing 3 times for 5 minutes each in 1X PBS. Sections were then blocked in blocking buffer (0.3% Triton X-100 in 1X PBS containing 10% normal goat serum) for 30 minutes at RT before staining with ab19347.

      Sections were also stained with DAPI nuclear stain (blue). Positive cells (green) were found at the base of the antral glands in the mouse stomach.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaSR antibody [5C10, ADD] (ab19347)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaSR antibody [5C10, ADD] (ab19347)

      Immunohistochemistry was performed on normal biopsies of deparaffinized Human kidney tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing CaSR ab19347 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

    • Flow Cytometry - Anti-CaSR antibody [5C10, ADD] (ab19347)
      Flow Cytometry - Anti-CaSR antibody [5C10, ADD] (ab19347)
      Overlay histogram showing SH-SY5Y cells stained with ab19347 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19347, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive result in 80% methanol (5 min) fixed SH-SY5Y cells used under the same conditions. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaSR antibody [5C10, ADD] (ab19347)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaSR antibody [5C10, ADD] (ab19347)

      Immunohistochemistry was performed on normal biopsies of deparaffinized Human brain tissue. To expose target proteins heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing CaSR ab19347 or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

    • Immunohistochemistry (Frozen sections) - Anti-CaSR antibody [5C10, ADD] (ab19347)
      Immunohistochemistry (Frozen sections) - Anti-CaSR antibody [5C10, ADD] (ab19347)This image is courtesy of an anonymous Abreview

      ab19347 at 1/100 staining rat brain (cerebral cortex) tissue sections by IHC-Fr. The tissue was paraformaldehyde fixed and blocked with serum before incubation with the primary antibody for 24 hours at 4°C. A biotinylated horse anti-mouse IgG was used as the secondary.

      See Abreview

    References (54)

    Publishing research using ab19347? Please let us know so that we can cite the reference in this datasheet.

    ab19347 has been referenced in 54 publications.

    • Tavanti GS  et al. Yes-Associated Protein 1 Is a Novel Calcium Sensing Receptor Target in Human Parathyroid Tumors. Int J Mol Sci 22:N/A (2021). PubMed: 33670622
    • Du SJ  et al. Astragaloside IV attenuates cerebral ischemia-reperfusion injury in rats through the inhibition of calcium-sensing receptor-mediated apoptosis. Int J Mol Med 47:302-314 (2021). PubMed: 33416112
    • Yuan C  et al. Calcium-Sensing Receptor Participates in High Glucose-Induced EndMT in Primary Human Aortic Endothelial Cells. Front Physiol 11:629542 (2020). PubMed: 33519531
    • Ostroverkhova DS  et al. Calcium-Sensing Receptor and Regulation of WNK Kinases in the Kidney. Cells 9:N/A (2020). PubMed: 32659887
    • Chen X  et al. Adropin regulates hepatic glucose production via PP2A/AMPK pathway in insulin-resistant hepatocytes. FASEB J 34:10056-10072 (2020). PubMed: 32579277
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 16 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-CaSR antibody [5C10, ADD]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Common marmoset Tissue sections (Cochlea)
    Permeabilization
    No
    Specification
    Cochlea
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jun 21 2022

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CaSR antibody [5C10, ADD]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rabbit Tissue sections (Joint knee)
    Antigen retrieval step
    Enzymatic - Buffer/Enzyme Used: Proteinase K
    Permeabilization
    No
    Specification
    Joint knee
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 24°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 28 2018

    Immunohistochemistry (Frozen sections) abreview for Anti-CaSR antibody [5C10, ADD]

     Average
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (blood vessels)
    Permeabilization
    No
    Specification
    blood vessels
    Blocking step
    1% BSA + 5% NGS as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Florian Guilbaud

    Verified customer

    Submitted Mar 24 2014

    Question

    Q: Antibody not working in WB but working in IHC-P?

    Read More
    Answer

    Thank you for contacting us.
    I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.
    I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.
    I look forward to receiving your reply.

    Read More

    Question

    Can you guarantee ab19347 in IHC-P?

    Read More
    Answer

    Thank you for your emails and telephone calls today.

    I am very pleased to hear you would like to accept our offer and test ab19347 in IHC-P. I have arranged the following discount code:

    DISCOUNT CODE: #####
    Expiration date: #####


    This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ab19347 in IHC-P and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.

    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Question

    Can you guarantee ab19347 in IHC-P?

    Read More
    Answer

    Thank you for your telephone enquiry earlier this morning.

    As discussed on the telephone, I am sorry to conrirm that to our knowledge, ab19347 has not been tested in WB. Therefore, I can offer a discount off a future purchase if you buy ab19347 now, test it in WB and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab19347 in WB. I will then send a discount code. This code must be issued before purchasing so please wait for my reply before ordering.

    2. Purchase ab19347 either by phone, fax, or online (www.abcam.com).

    3. Test it in WB.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

    Please remember that submission of the Abreview is sufficient for the discount code to become active.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab19347 turns out to be unsuitable for WB, you will still receive the discount on your next purchase after your Abreview has been submitted.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    Read More

    Question

    will test ab19347 in chicken: homology: 100%
    will test ab71842 in IHC-Fr and chicken: homology 84%

    Read More
    Answer

    DISCOUNT CODE: xxx for ab19347
    DISCOUNT CODE: xxx for ab71842
    Expiration date: xxx

    I am very pleased to hear you would like to accept our offer and test ab19347 and ab71842in chicken and/or IHC-Fr. Each code will give you: 1 free PRIMARY ANTIBODYbefore the expiration date. To redeem this offer, please submit an Abreview for chicken and/or IHC-Frand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

    Read More

    Question

    Could you recomend blocking peptide for ab18200?

    Read More
    Answer

    Thank you for contacting us.

    The catalogue number of blocking peptide is ab18608.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

    Read More

    Question

    Dear Sir/Madam, I was wondering if your CaSR antibody is also reactive against porcine CaSR? Would you advise me to try this? Many thanks! Kind regards,

    Read More
    Answer

    Thank you for your enquiry and your interest in our products.

    I can confirm it for you that this antibody (ab19347: Anti-Calcium Sensing Receptor antibody) has been tested for cross-reaction with rat, cow, human. Based on blast search (using Uniprot) there is a very high homology between human and bovine aa for Calcium Sensing Receptor. Unfortunately, there seems to be no information on procine CaSR on the public domain, you may wish to check this website for further information:

    http://www.uniprot.org/blast/uniprot/2012032742K5NXXKK7

    If you wish to test this antibody in pig samples, I could offer you a testing discount. The Terms and Conditions of this offer can be found at: https://www.abcam.com/collaborationdiscount.

    I hope this helps and if I can assist further, please do not hesitate to contact me.

    Read More

    Question

    Dear xxxx/ Abcam support, Thank you for the detailed information given. I have considered the options and have decided to proceed and test ab18200 in mouse samples. Please send the discount code.   Thank you once again!   Kind Regards,  

    Read More
    Answer

    Thank you for contacting us again. I am very pleased to hear you are choosing to test ab18200 with your mouse samples. I have generated the discount code, this will entitle you to one free primary antibody before the expiration date: DISCOUNT CODE: xxxxxx Expiration date: 2012-03-02 To redeem this offer, please purchase ab18200, test it with IHC-P with your mouse samples then submit an Abreview. Please remember to include the discount code above in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests either way. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. I look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount

    Read More

    1-10 of 16 Abreviews or Q&A

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