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    products/primary-antibodies/catalase-antibody-12c2db9-peroxisome-marker-ab110292.pdf

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Tags & Cell Markers Subcellular Markers Organelles Peroxisome
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Validated using a knockout cell line

Anti-Catalase antibody [12C2DB9] - Peroxisome Marker (ab110292)

  • Datasheet
  • SDS
Reviews (3)Q&A (1)References (3)

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Flow Cytometry - Anti-Catalase antibody [12C2DB9] - Peroxisome Marker (ab110292)
  • Immunocytochemistry - Anti-Catalase antibody [12C2DB9] - Peroxisome Marker (ab110292)
  • Immunoprecipitation - Anti-Catalase antibody [12C2DB9] - Peroxisome Marker (ab110292)
  • Flow Cytometry - Anti-Catalase antibody [12C2DB9] - Peroxisome Marker (ab110292)

Key features and details

  • Mouse monoclonal [12C2DB9] to Catalase - Peroxisome Marker
  • Suitable for: ICC, IP, Flow Cyt
  • Knockout validated
  • Reacts with: Rat, Cow, Human
  • Isotype: IgG1

You may also be interested in

Protein
Product image
Recombinant Catalase protein (Active) (ab219092)
Knockout
Product image
Human CAT (Catalase) knockout HeLa cell line (ab265250)
Primary
Product image
Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)

View more associated products

Overview

  • Product name

    Anti-Catalase antibody [12C2DB9] - Peroxisome Marker
    See all Catalase primary antibodies
  • Description

    Mouse monoclonal [12C2DB9] to Catalase - Peroxisome Marker
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC, IP, Flow Cytmore details
  • Species reactivity

    Reacts with: Rat, Cow, Human
  • Immunogen

    Tissue, cells or virus. This information is considered to be commercially sensitive.

  • Positive control

    • HeLa cells, HepG2 cells, HL60 cells, Cow heart, Rat liver Flow cyt: HAP1-WT cells.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Product was previously marketed under the MitoSciences sub-brand.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.5
    Preservative: 0.02% Sodium azide
    Constituent: HEPES buffered saline
  • Concentration information loading...
  • Purity

    Ammonium Sulphate Precipitation
  • Purification notes

    Purity near homogeneity as judge by SDS-PAGE. The antibody was produced in-vitro using hybridomas grown in serum-free medium and then purified by biochemical fractionation.
  • Clonality

    Monoclonal
  • Clone number

    12C2DB9
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Peroxisome
    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Heat Shock Proteins
    • Cardiovascular
    • Heart
    • Cardiac metabolism
    • Cancer
    • Cancer Metabolism
    • Cellular metabolic process
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Antioxidants
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Catalase protein (Active) (ab219092)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab110292 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC (1)
Use a concentration of 5 µg/ml.
IP
Use at an assay dependent concentration.
Flow Cyt
Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Notes
ICC
Use a concentration of 5 µg/ml.
IP
Use at an assay dependent concentration.
Flow Cyt
Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
  • Involvement in disease

    Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
  • Sequence similarities

    Belongs to the catalase family.
  • Post-translational
    modifications

    The N-terminus is blocked.
  • Cellular localization

    Peroxisome.
  • Target information above from: UniProt accession P04040 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 531682 Cow
    • Entrez Gene: 847 Human
    • Entrez Gene: 24248 Rat
    • Omim: 115500 Human
    • SwissProt: P00432 Cow
    • SwissProt: P04040 Human
    • SwissProt: P04762 Rat
    • Unigene: 502302 Human
    • Unigene: 3001 Rat
    see all
  • Alternative names

    • Cas1 antibody
    • CAT antibody
    • CATA_HUMAN antibody
    • Catalase antibody
    • Cs1 antibody
    • MGC138422 antibody
    • MGC138424 antibody
    see all

Images

  • Flow Cytometry - Anti-Catalase antibody [12C2DB9] - Peroxisome Marker (ab110292)
    Flow Cytometry - Anti-Catalase antibody [12C2DB9] - Peroxisome Marker (ab110292)

    Overlay histogram showing HAP1 wildtype (green line) and HAP1-CAT knockout cells (red line) stained with ab110292. The cells were fixed with 4% formaldehyde (10 min)  and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab110292, 1µg/ml) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) presorbed (ab150117) at 1/2000 dilution for 30 min at 22°C.

    A mouse IgG1 isotype control antibody  (ab170190) was used at the same concentration and conditions as the primary antibody (HAP1 wildtype - black line, HAP1-CAT  knockout - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

    Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

  • Immunocytochemistry - Anti-Catalase antibody [12C2DB9] - Peroxisome Marker (ab110292)
    Immunocytochemistry - Anti-Catalase antibody [12C2DB9] - Peroxisome Marker (ab110292)
    Immunocytochemical staining of Human HeLa cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with the ab110292 (5 µg/ml) for 2 hours at room temperature or over night at 4°C. The secondary antibody, Alexa Fluor® 488 goat anti-mouse IgG (green), was used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). The target protein locates mainly in peroxisomes.
  • Immunoprecipitation - Anti-Catalase antibody [12C2DB9] - Peroxisome Marker (ab110292)
    Immunoprecipitation - Anti-Catalase antibody [12C2DB9] - Peroxisome Marker (ab110292)
    Immunoprecipitation of Catalase using ab110292 from:
    Lane 1: HepG2 cells
    Lane 2: Cow heart sample
    Lane 3: Rat liver sample

    Predicted band size is 60 kDa.
  • Flow Cytometry - Anti-Catalase antibody [12C2DB9] - Peroxisome Marker (ab110292)
    Flow Cytometry - Anti-Catalase antibody [12C2DB9] - Peroxisome Marker (ab110292)
    Flow cytometric analysis of HL-60 cells using ab110292 at 1 µg/mL, (blue) or an isotype control (red).

Protocols

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (3)

Publishing research using ab110292? Please let us know so that we can cite the reference in this datasheet.

ab110292 has been referenced in 3 publications.

  • Covill-Cooke C  et al. Peroxisomal fission is modulated by the mitochondrial Rho-GTPases, Miro1 and Miro2. EMBO Rep 21:e49865 (2020). PubMed: 31894645
  • Chang CL  et al. Spastin tethers lipid droplets to peroxisomes and directs fatty acid trafficking through ESCRT-III. J Cell Biol 218:2583-2599 (2019). PubMed: 31227594
  • Gut G  et al. Multiplexed protein maps link subcellular organization to cellular states. Science 361:N/A (2018). PubMed: 30072512

Customer reviews and Q&As

Show All Reviews Q&A
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1-4 of 4 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Catalase antibody [12C2DB9] - Peroxisome Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
African green monkey Cell (COS-7)
Permeabilization
Yes - Triton
Specification
COS-7
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 06 2022

Immunocytochemistry abreview for Anti-Catalase antibody [12C2DB9] - Peroxisome Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
African green monkey Cultured Cells (Vero cells)
Permeabilization
Yes - Triton X-100 0.2%
Specification
Vero cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 07 2022

Immunocytochemistry/ Immunofluorescence abreview for Anti-Catalase antibody [12C2DB9] - Peroxisome Marker

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HFF cells)
Permeabilization
Yes - Triton X-100
Specification
HFF cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Dec 23 2021

Question

I was interested in buying some Catalase antibody and was wondering what kind of Kd values they have towards bovine catalase?

Read More

Abcam community

Verified customer

Asked on Mar 05 2012

Answer

Thank you very much for contacting us.

Unfortunately, the dissociation constant KD is not routinely determined and we therefore cannot provide this information on our antibodies against bovine catalase.

Having said this, I am happy to let you know that we have at least six anti-catalase antibodies that have been tested with and are guaranteed to work with bovine samples. These include for example the rabbit polyclonal ab1877 or the mouse monoclonal ab110292:

Click here (or use the following: https://www.abcam.com/Catalase-antibody-ab1877.html).

Click here (or use the following: https://www.abcam.com/Catalase-antibody-12C2DB9-Peroxisome-Marker-ab110292.html).

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase (www.abcam.com/Abpromise).

If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information.

We aim to provide as much information as possible to customers, so I am sorry that this has not been possible on this occasion. Should you have any further questions, please do not hesitate to contact us.

Read More

Abcam Scientific Support

Answered on Mar 05 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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