Anti-Catalase antibody [12C2DB9] - Peroxisome Marker (ab110292)
![Flow Cytometry - Anti-Catalase antibody [12C2DB9] - Peroxisome Marker (ab110292)](https://www.abcam.com/ps/products/110/ab110292/Images/ab110292-306535-anti-catalase-antibody-12c2db9-peroxisome-marker-flow-cytometry.jpg "Flow Cytometry - Anti-Catalase antibody [12C2DB9] - Peroxisome Marker (ab110292)")
Key features and details
- Mouse monoclonal [12C2DB9] to Catalase - Peroxisome Marker
- Suitable for: ICC, IP, Flow Cyt
- Knockout validated
- Reacts with: Rat, Cow, Human
- Isotype: IgG1
Overview
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Product name
Anti-Catalase antibody [12C2DB9] - Peroxisome Marker
See all Catalase primary antibodies -
Description
Mouse monoclonal [12C2DB9] to Catalase - Peroxisome Marker -
Host species
Mouse -
Tested applications
Suitable for: ICC, IP, Flow Cytmore details -
Species reactivity
Reacts with: Rat, Cow, Human -
Immunogen
Tissue, cells or virus. This information is considered to be commercially sensitive.
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Positive control
- HeLa cells, HepG2 cells, HL60 cells, Cow heart, Rat liver Flow cyt: HAP1-WT cells.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Product was previously marketed under the MitoSciences sub-brand.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
Purity
Ammonium Sulphate Precipitation -
Purification notes
Purity near homogeneity as judge by SDS-PAGE. The antibody was produced in-vitro using hybridomas grown in serum-free medium and then purified by biochemical fractionation. -
Clonality
Monoclonal -
Clone number
12C2DB9 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110292 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC | (1) |
Use a concentration of 5 µg/ml.
|
| IP |
Use at an assay dependent concentration.
|
|
| Flow Cyt |
Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
ICC
Use a concentration of 5 µg/ml. |
|
IP
Use at an assay dependent concentration. |
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Flow Cyt
Use a concentration of 1 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. -
Involvement in disease
Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions. -
Sequence similarities
Belongs to the catalase family. -
Post-translational
modificationsThe N-terminus is blocked. -
Cellular localization
Peroxisome. - Information by UniProt
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Database links
- Entrez Gene: 531682 Cow
- Entrez Gene: 847 Human
- Entrez Gene: 24248 Rat
- Omim: 115500 Human
- SwissProt: P00432 Cow
- SwissProt: P04040 Human
- SwissProt: P04762 Rat
- Unigene: 502302 Human
see all -
Alternative names
- Cas1 antibody
- CAT antibody
- CATA_HUMAN antibody
see all
Images
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Flow Cytometry - Anti-Catalase antibody [12C2DB9] - Peroxisome Marker (ab110292)
Overlay histogram showing HAP1 wildtype (green line) and HAP1-CAT knockout cells (red line) stained with ab110292. The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab110292, 1µg/ml) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) presorbed (ab150117) at 1/2000 dilution for 30 min at 22°C.
A mouse IgG1 isotype control antibody (ab170190) was used at the same concentration and conditions as the primary antibody (HAP1 wildtype - black line, HAP1-CAT knockout - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).
Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. -
![Immunocytochemistry - Anti-Catalase antibody [12C2DB9] - Peroxisome Marker (ab110292)](https://www.abcam.com/ps/products/110/ab110292/Images/ab110292-123524-anti-catalase-antibody-12c2db9-peroxisome-marker-immunocytochemistry.gif)
Immunocytochemistry - Anti-Catalase antibody [12C2DB9] - Peroxisome Marker (ab110292)Immunocytochemical staining of Human HeLa cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with the ab110292 (5 µg/ml) for 2 hours at room temperature or over night at 4°C. The secondary antibody, Alexa Fluor® 488 goat anti-mouse IgG (green), was used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). The target protein locates mainly in peroxisomes. -
![Immunoprecipitation - Anti-Catalase antibody [12C2DB9] - Peroxisome Marker (ab110292)](https://www.abcam.com/ps/products/110/ab110292/Images/ab110292-123523-anti-catalase-antibody-12c2db9-peroxisome-marker-immunoprecipitation.gif)
Immunoprecipitation - Anti-Catalase antibody [12C2DB9] - Peroxisome Marker (ab110292)Immunoprecipitation of Catalase using ab110292 from:
Lane 1: HepG2 cells
Lane 2: Cow heart sample
Lane 3: Rat liver sample
Predicted band size is 60 kDa. -
![Flow Cytometry - Anti-Catalase antibody [12C2DB9] - Peroxisome Marker (ab110292)](https://www.abcam.com/ps/products/110/ab110292/Images/ab110292-123522-anti-catalase-antibody-12c2db9-peroxisome-marker-flow-cytometry.jpg)
Flow Cytometry - Anti-Catalase antibody [12C2DB9] - Peroxisome Marker (ab110292)Flow cytometric analysis of HL-60 cells using ab110292 at 1 µg/mL, (blue) or an isotype control (red).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab110292 has been referenced in 3 publications.
- Covill-Cooke C et al. Peroxisomal fission is modulated by the mitochondrial Rho-GTPases, Miro1 and Miro2. EMBO Rep 21:e49865 (2020). PubMed: 31894645
- Chang CL et al. Spastin tethers lipid droplets to peroxisomes and directs fatty acid trafficking through ESCRT-III. J Cell Biol 218:2583-2599 (2019). PubMed: 31227594
- Gut G et al. Multiplexed protein maps link subcellular organization to cellular states. Science 361:N/A (2018). PubMed: 30072512