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    products/primary-antibodies/catalase-antibody-ep1929y-bsa-and-azide-free-ab227116.pdf

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Tags & Cell Markers Subcellular Markers Organelles Peroxisome
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Catalase antibody [EP1929Y] - BSA and Azide free (ab227116)

  • Datasheet
  • Certificate of Compliance
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Western blot - Anti-Catalase antibody [EP1929Y] - BSA and Azide free (ab227116)
  • Western blot - Anti-Catalase antibody [EP1929Y] - BSA and Azide free (ab227116)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Catalase antibody [EP1929Y] - BSA and Azide free (ab227116)
  • Immunocytochemistry/ Immunofluorescence - Anti-Catalase antibody [EP1929Y] - BSA and Azide free (ab227116)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Catalase antibody [EP1929Y] - BSA and Azide free (ab227116)
  • Anti-Catalase antibody [EP1929Y] - BSA and Azide free (ab227116)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1929Y] to Catalase - BSA and Azide free
  • Suitable for: IHC-P, WB, ICC/IF
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Unconjugated

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Protein
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Recombinant Catalase protein (Active) (ab219092)
Primary
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Anti-TIMM23/TIM23 antibody (ab230253)
Knockout
Product image
Human CAT (Catalase) knockout HeLa cell line (ab261816)

View more associated products

Overview

  • Product name

    Anti-Catalase antibody [EP1929Y] - BSA and Azide free
    See all Catalase primary antibodies
  • Description

    Rabbit monoclonal [EP1929Y] to Catalase - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa cell lysate. IHC-P: human brain tissue. ICC/IF: HeLa cells.
  • General notes

    ab227116 is the carrier-free version of ab76024.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.20
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1929Y
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Peroxisome
    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Heat Shock Proteins
    • Cardiovascular
    • Heart
    • Cardiac metabolism
    • Cancer
    • Cancer Metabolism
    • Cellular metabolic process
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Antioxidants

Associated products

  • Alternative Versions

    • Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Conjugation kits

    • Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (ab201795)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human CAT (Catalase) knockout HeLa cell line (ab265250)
  • KO cell lysates

    • Human CAT (Catalase) knockout HeLa cell lysate (ab256859)
  • Recombinant Protein

    • Recombinant Catalase protein (Active) (ab219092)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab227116 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
ICC/IF
Use at an assay dependent concentration.
Notes
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
ICC/IF
Use at an assay dependent concentration.
Application notes
Is unsuitable for Flow Cyt or IP.

Target

  • Function

    Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
  • Involvement in disease

    Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
  • Sequence similarities

    Belongs to the catalase family.
  • Post-translational
    modifications

    The N-terminus is blocked.
  • Cellular localization

    Peroxisome.
  • Target information above from: UniProt accession P04040 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 847 Human
    • Omim: 115500 Human
    • SwissProt: P04040 Human
    • Unigene: 502302 Human
    • Alternative names

      • Cas1 antibody
      • CAT antibody
      • CATA_HUMAN antibody
      • Catalase antibody
      • Cs1 antibody
      • MGC138422 antibody
      • MGC138424 antibody
      see all

    Images

    • Western blot - Anti-Catalase antibody [EP1929Y] - BSA and Azide free (ab227116)
      Western blot - Anti-Catalase antibody [EP1929Y] - BSA and Azide free (ab227116)
      All lanes : Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024) at 1/10000 dilution

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : CAT knockout HeLa cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 60 kDa
      Observed band size: 60 kDa



      This data was developed using the same antibody clone in a different buffer formulation (ab76024).

      Lanes 1-2: Merged signal (red and green). Green - ab76024 observed at 60 kDa. Red - loading control ab8245 observed at 37 kDa.

       ab76024 Anti-Catalase antibody [EP1929Y] - Peroxisome Marker was shown to specifically react with Catalase in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265250 (knockout cell lysate ab256859) was used. Wild-type and Catalase knockout samples were subjected to SDS-PAGE. ab76024 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 10000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

       

       

    • Western blot - Anti-Catalase antibody [EP1929Y] - BSA and Azide free (ab227116)
      Western blot - Anti-Catalase antibody [EP1929Y] - BSA and Azide free (ab227116)
      All lanes : Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024) at 1/5000 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : CAT knockout HAP1 whole cell lysate
      Lane 3 : HeLa whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 60 kDa



      This WB data was generated using the same anti-Catalase antibody clone, EP1929Y, in a different buffer formulation (cat# ab76024).

      Lanes 1 - 3: Merged signal (red and green). Green - ab76024 observed at 60 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab76024 was shown to specifically react with CAT when CAT knockout samples were used. Wild-type and CAT knockout samples were subjected to SDS-PAGE. Ab76024 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Catalase antibody [EP1929Y] - BSA and Azide free (ab227116)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Catalase antibody [EP1929Y] - BSA and Azide free (ab227116)

      Immunohistochemical analysis of Paraffin-embedded human bladder cancer tissue sections labeling Catalase with ab76024 at 1/1000. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using ab93684 (Tris/EDTA buffer, pH 9.0).

      Granular cytoplasmic staining on human bladder cancer.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76024).

    • Immunocytochemistry/ Immunofluorescence - Anti-Catalase antibody [EP1929Y] - BSA and Azide free (ab227116)
      Immunocytochemistry/ Immunofluorescence - Anti-Catalase antibody [EP1929Y] - BSA and Azide free (ab227116)

      Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) labeling Catalase ab76024 at 1/100. Cells were fixed with 100% Methanol. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. 

      ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/100 was used as counterstain antibody.

      Confocal image showing membranous staining in HeLa cells.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76024).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Catalase antibody [EP1929Y] - BSA and Azide free (ab227116)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Catalase antibody [EP1929Y] - BSA and Azide free (ab227116)

      Immunohistochemical staining of Catalase in paraffin embedded human normal brain tissue using ab76024 at a 1/100 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76024).

    • Anti-Catalase antibody [EP1929Y] - BSA and Azide free (ab227116)
      Anti-Catalase antibody [EP1929Y] - BSA and Azide free (ab227116)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (2)

    Publishing research using ab227116? Please let us know so that we can cite the reference in this datasheet.

    ab227116 has been referenced in 2 publications.

    • Ma HP Hydrogen peroxide stimulates the epithelial sodium channel through a phosphatidylinositide 3-kinase-dependent pathway. J Biol Chem 286:32444-53 (2011). WB ; Xenopus laevis . PubMed: 21795700
    • Kemp K  et al. Mesenchymal stem cells restore frataxin expression and increase hydrogen peroxide scavenging enzymes in friedreich ataxia fibroblasts. PLoS One 6:e26098 (2011). WB ; Human . PubMed: 22016819

    Customer reviews and Q&As

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