Recombinant Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)
![Western blot - Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)](https://www.abcam.com/ps/products/76/ab76024/Images/ab76024-388712-anti-catalase-antibody-ep1929y-peroxisome-marker-western-blot-hela-lysates.jpg "Western blot - Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1929Y] to Catalase - Peroxisome Marker
- Suitable for: ICC/IF, WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Catalase antibody [EP1929Y] - Peroxisome Marker
See all Catalase primary antibodies -
Description
Rabbit monoclonal [EP1929Y] to Catalase - Peroxisome Marker -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human -
Immunogen
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Positive control
- WB: HeLa cell lysate. IHC-P: human brain tissue, human bladder cancer tissue. ICC/IF: HeLa cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP1929Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76024 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/100 - 1/250.
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| WB | (1) |
1/10000 - 1/20000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| Notes |
|---|
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ICC/IF
1/100 - 1/250. |
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WB
1/10000 - 1/20000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa). |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. -
Involvement in disease
Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions. -
Sequence similarities
Belongs to the catalase family. -
Post-translational
modificationsThe N-terminus is blocked. -
Cellular localization
Peroxisome. - Information by UniProt
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Database links
- Entrez Gene: 847 Human
- Omim: 115500 Human
- SwissProt: P04040 Human
- Unigene: 502302 Human
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Alternative names
- Cas1 antibody
- CAT antibody
- CATA_HUMAN antibody
see all
Images
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Western blot - Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)All lanes : Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024) at 1/10000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : CAT knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 60 kDa
Observed band size: 60 kDaLanes 1-2: Merged signal (red and green). Green - ab76024 observed at 60 kDa. Red - loading control ab8245 observed at 37 kDa.
ab76024 Anti-Catalase antibody [EP1929Y] - Peroxisome Marker was shown to specifically react with Catalase in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265250 (knockout cell lysate ab256859) was used. Wild-type and Catalase knockout samples were subjected to SDS-PAGE. ab76024 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 10000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)](https://www.abcam.com/ps/products/76/ab76024/Images/ab76024-354840-recombinant-anti-catalase-antibody-ep1929y-peroxisome-marker-immunohistochemistry-human-bladder-cancer.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)Immunohistochemical analysis of Paraffin-embedded human bladder cancer tissue sections labeling Catalase with ab76024 at 1/1000. Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using ab93684 (Tris/EDTA buffer, pH 9.0).
Granular cytoplasmic staining on human bladder cancer.
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![Western blot - Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)](https://www.abcam.com/ps/products/76/ab76024/Images/ab76024-282492-anti-catalase-antibody-ep1929y-peroxisome-marker-western-blot.jpg)
Western blot - Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)All lanes : Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : CAT knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 60 kDaLanes 1 - 3: Merged signal (red and green). Green - ab76024 observed at 60 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab76024 was shown to specifically react with CAT when CAT knockout samples were used. Wild-type and CAT knockout samples were subjected to SDS-PAGE. Ab76024 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)](https://www.abcam.com/ps/products/76/ab76024/Images/ab76024-354839-recombinant-anti-catalase-antibody-ep1929y-peroxisome-marker-immunocytochemistry-immunofluorescence-human-hela.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) labeling Catalase ab76024 at 1/100. Cells were fixed with 100% Methanol. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.
ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/100 was used as counterstain antibody.
Confocal image showing membranous staining in HeLa cells.
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![Western blot - Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)](https://www.abcam.com/ps/products/76/ab76024/Images/ab76024-91326-anti-catalase-antibody-ep1929y-peroxisome-marker-western-blot.jpg)
Western blot - Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024) at 1/20000 dilution + HeLa cell lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)](https://www.abcam.com/ps/products/76/ab76024/Images/ab76024-91325-anti-catalase-antibody-ep1929y-peroxisome-marker-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)Immunohistochemical staining of Catalase in paraffin embedded human normal brain tissue using ab76024 at a 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)](https://www.abcam.com/ps/products/76/ab76024/Images/ab76024-8-benefits-of-recombinant-antibodies.png)
Anti-Catalase antibody [EP1929Y] - Peroxisome Marker (ab76024)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (18)
ab76024 has been referenced in 18 publications.
- Flory S et al. Uptake and time-dependent subcellular localization of native and micellar curcumin in intestinal cells. Biofactors 48:897-907 (2022). PubMed: 35170815
- Tripp TR et al. Time course and fibre type-dependent nature of calcium-handling protein responses to sprint interval exercise in human skeletal muscle. J Physiol 600:2897-2917 (2022). PubMed: 35556249
- Costanzo-Garvey DL et al. Prostate cancer addiction to oxidative stress defines sensitivity to anti-tumor neutrophils. Clin Exp Metastasis 39:641-659 (2022). PubMed: 35604506
- Yang C et al. TEOA Promotes Autophagic Cell Death via ROS-Mediated Inhibition of mTOR/p70S6k Signaling Pathway in Pancreatic Cancer Cells. Front Cell Dev Biol 9:734818 (2021). PubMed: 34692691
- Nørgård MØ et al. Phenformin Attenuates Renal Injury in Unilateral Ureteral Obstructed Mice without Affecting Immune Cell Infiltration. Pharmaceutics 12:N/A (2020). PubMed: 32224876