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    products/primary-antibodies/catalase-antibody-epr1928y-ab76110.pdf

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Tags & Cell Markers Subcellular Markers Organelles Peroxisome
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Catalase antibody [EPR1928Y] (ab76110)

  • Datasheet
  • SDS
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Western blot - Anti-Catalase antibody [EPR1928Y] (ab76110)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Catalase antibody [EPR1928Y] (ab76110)
  • Western blot - Anti-Catalase antibody [EPR1928Y] (ab76110)
  • Anti-Catalase antibody [EPR1928Y] (ab76110)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1928Y] to Catalase
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

You may also be interested in

Protein
Product image
Recombinant Catalase protein (Active) (ab219092)
Knockout
Product image
Human CAT (Catalase) knockout HeLa cell line (ab261816)
Assay
Product image
Catalase Activity Assay Kit (Colorimetric/Fluorometric) (ab83464)

View more associated products

Overview

  • Product name

    Anti-Catalase antibody [EPR1928Y]
    See all Catalase primary antibodies
  • Description

    Rabbit monoclonal [EPR1928Y] to Catalase
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Catalase (N terminal). The exact sequence is proprietary.

  • Positive control

    • TF1, HeLa, Jurkat and HepG2 whole cell lysate (ab7900); human liver tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR1928Y
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Peroxisome
    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Heat Shock Proteins
    • Cardiovascular
    • Heart
    • Cardiac metabolism
    • Cancer
    • Cancer Metabolism
    • Cellular metabolic process
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Antioxidants
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Alternative Versions

    • Anti-Catalase antibody [EPR1928Y] - BSA and Azide free (ab247440)
  • Compatible Secondaries

    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Catalase protein (Active) (ab219092)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab76110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
WB
1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IP.

Target

  • Function

    Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.
  • Involvement in disease

    Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.
  • Sequence similarities

    Belongs to the catalase family.
  • Post-translational
    modifications

    The N-terminus is blocked.
  • Cellular localization

    Peroxisome.
  • Target information above from: UniProt accession P04040 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 847 Human
    • Entrez Gene: 12359 Mouse
    • Entrez Gene: 24248 Rat
    • Omim: 115500 Human
    • SwissProt: P04040 Human
    • SwissProt: P24270 Mouse
    • SwissProt: P04762 Rat
    • Unigene: 502302 Human
    • Unigene: 4215 Mouse
    • Unigene: 3001 Rat
    see all
  • Alternative names

    • Cas1 antibody
    • CAT antibody
    • CATA_HUMAN antibody
    • Catalase antibody
    • Cs1 antibody
    • MGC138422 antibody
    • MGC138424 antibody
    see all

Images

  • Western blot - Anti-Catalase antibody [EPR1928Y] (ab76110)
    Western blot - Anti-Catalase antibody [EPR1928Y] (ab76110)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Catalase knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: Jurkat cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab76110 observed at 60 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab76110 was shown to specifically react with Catalase when Catalase knockout samples were used. Wild-type and Catalase knockout samples were subjected to SDS-PAGE. ab76110 and ab8245 (loading control to GAPDH) were diluted at 1/500 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW)  preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD)  preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Catalase antibody [EPR1928Y] (ab76110)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Catalase antibody [EPR1928Y] (ab76110)

    ab76110 at 1/100-1/250 dilution staining Catalase in human liver by Immunohistochemistry, Paraffin-embedded tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-Catalase antibody [EPR1928Y] (ab76110)
    Western blot - Anti-Catalase antibody [EPR1928Y] (ab76110)
    All lanes : Anti-Catalase antibody [EPR1928Y] (ab76110) at 1/1000 dilution

    Lane 1 : TF cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 60 kDa
    Observed band size: 60 kDa

  • Anti-Catalase antibody [EPR1928Y] (ab76110)
    Anti-Catalase antibody [EPR1928Y] (ab76110)

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (14)

Publishing research using ab76110? Please let us know so that we can cite the reference in this datasheet.

ab76110 has been referenced in 14 publications.

  • Li H  et al. Exosomes containing miR-451a is involved in the protective effect of cerebral ischemic preconditioning against cerebral ischemia and reperfusion injury. CNS Neurosci Ther 27:564-576 (2021). PubMed: 33533575
  • Zheng T  et al. Dexmedetomidine suppresses bupivacaine-induced parthanatos in human SH-SY5Y cells via the miR-7-5p/PARP1 axis-mediated ROS. Naunyn Schmiedebergs Arch Pharmacol 394:783-796 (2021). PubMed: 32989562
  • Monteiro-Alfredo T  et al. Hypoglycaemic and Antioxidant Properties of Acrocomia aculeata (Jacq.) Lodd Ex Mart. Extract Are Associated with Better Vascular Function of Type 2 Diabetic Rats. Nutrients 13:N/A (2021). PubMed: 34445015
  • Xiang L  et al. Curcumin analog A13 alleviates oxidative stress by activating Nrf2/ARE pathway and ameliorates fibrosis in the myocardium of high-fat-diet and streptozotocin-induced diabetic rats. Diabetol Metab Syndr 12:1 (2020). PubMed: 31921358
  • You L  et al. Enterovirus 71 induces neural cell apoptosis and autophagy through promoting ACOX1 downregulation and ROS generation. Virulence 11:537-553 (2020). PubMed: 32434419
View all Publications for this product

Customer reviews and Q&As

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