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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211)

Reviews (3) Submit a question References (19)
Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211)
  • Immunocytochemistry/ Immunofluorescence - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211)
  • Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211)
  • Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211)
  • Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211)
  • Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211)
  • Immunocytochemistry/ Immunofluorescence - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211)
  • Flow Cytometry (Intracellular) - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211)
  • Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20198] to Catalase - Peroxisome Marker
  • Suitable for: WB, ICC/IF, Flow Cyt (Intra)
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Catalase antibody [EPR20198] - Peroxisome Marker
    See all Catalase primary antibodies

  • Description

    Rabbit monoclonal [EPR20198] to Catalase - Peroxisome Marker

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cyt (Intra)more details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse brain, heart, spleen and kidney lysates; rat heart and brain lysates; C6, PC-12, NIH/3T3, HepG2, 293 and C2C12 whole cell lysates; Human fetal brain, fetal heart and fetal kidney lysates. ICC/IF: C2C12 and NIH/3T3 cells. Flow Cyt (intra): C2C12 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab209211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
1/2000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
ICC/IF
1/100.
Flow Cyt (Intra)
1/60.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Notes
WB
1/2000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
ICC/IF
1/100.
Flow Cyt (Intra)
1/60.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.

  • Involvement in disease

    Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions.

  • Sequence similarities

    Belongs to the catalase family.

  • Post-translational
    modifications

    The N-terminus is blocked.

  • Cellular localization

    Peroxisome.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Cas1 antibody
    • CAT antibody
    • CATA_HUMAN antibody
    • Catalase antibody
    • Cs1 antibody
    • MGC138422 antibody
    • MGC138424 antibody
    see all

Images

  • Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211)
    Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211)
    All lanes : Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211) at 1/2000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : CAT knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 60 kDa
    Observed band size: 60 kDa



    Lanes 1-2: Merged signal (red and green). Green - ab209211 observed at 60 kDa. Red - loading control ab8245 observed at 37 kDa.

    ab209211 Anti-Catalase antibody [EPR20198] - Peroxisome Marker was shown to specifically react with Catalase in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265250 (knockout cell lysate ab256859) was used. Wild-type and Catalase knockout samples were subjected to SDS-PAGE. ab209211 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 2000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Immunocytochemistry/ Immunofluorescence - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211)
    Immunocytochemistry/ Immunofluorescence - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C2C12 (Mouse myoblast cell line) cells labeling Catalase with ab209211 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on C2C12 cell line. The nuclear counterstain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.

  • Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211)
    Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211)
    All lanes : Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211) at 1/2000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : CAT knockout HAP1 whole cell lysate
    Lane 3 : Hek293 whole cell lysate
    Lane 4 : HepG2 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 60 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab209211 observed at 60 kDa. Red - loading control, ab8245, observed at 37 kDa.


    ab209211 was shown to specifically react with CAT in wild-type HAP1 cells. No band was observed when CAT knockout samples were examined. Wild-type and CAT knockout samples were subjected to SDS-PAGE. Ab209211 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/2000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211)
    Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211)
    All lanes : Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211) at 1/2000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Mouse spleen lysate
    Lane 4 : Mouse kidney lysate
    Lane 5 : Rat heart lysate
    Lane 6 : Rat brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 60 kDa
    Observed band size: 60 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1-3: 5 seconds; Lane 4/5: 1 second; Lane 6: 3 seconds.

  • Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211)
    Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211)
    All lanes : Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211) at 1/2000 dilution

    Lane 1 : C6 (Rat glial tumor cell line) whole cell lysate
    Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 3 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
    Lane 5 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 6 : C2C12 (Mouse myoblast cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 60 kDa
    Observed band size: 60 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 1 second; Lane 2/3/6: 3 seconds; Lane 4/5: 30 seconds.

  • Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211)
    Western blot - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211)
    All lanes : Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211) at 1/2000 dilution

    Lane 1 : Human fetal brain lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : Human fetal kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 60 kDa
    Observed band size: 60 kDa


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211)
    Immunocytochemistry/ Immunofluorescence - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211)

    ImmunoFluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryonic fibroblast cell line) cells labeling Catalase with ab209211 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on NIH/3T3 cell line. The nuclear counterstain is DAPI (blue). 

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red). 

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution. 

     

     

  • Flow Cytometry (Intracellular) - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211)
    Flow Cytometry (Intracellular) - Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed C2C12 (Mouse myoblast cell line) cells labeling Catalase with ab209211 at 1/60 dilution (red) compared withRabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. 

     

     

  • Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211)
    Anti-Catalase antibody [EPR20198] - Peroxisome Marker (ab209211)

References (19)

Publishing research using ab209211? Please let us know so that we can cite the reference in this datasheet.

ab209211 has been referenced in 19 publications.

  • Zhou X  et al. Melatonin Prevents Cartilage Degradation in Early-Stage Osteoarthritis Through Activation of miR-146a/NRF2/HO-1 Axis. J Bone Miner Res 37:1056-1072 (2022). PubMed: 35147250
  • Li J & Xu S Tilianin attenuates MPP+-induced oxidative stress and apoptosis of dopaminergic neurons in a cellular model of Parkinson's disease. Exp Ther Med 23:293 (2022). PubMed: 35340873
  • Wang G  et al. Uncovering the Neuroprotective Effect of Hedysarum multijugum Maxim-Chuanxiong Rhizoma Compound on Cerebral Infarction through Quantitative Proteomics. Evid Based Complement Alternat Med 2022:5241902 (2022). PubMed: 35378910
  • Kumar R  et al. Induction of reactive oxygen species by mechanical stretch drives endothelin production in neonatal pig renal epithelial cells. Redox Biol 55:102394 (2022). PubMed: 35841629
  • Deng J  et al. Cerium oxide-based nanozyme suppresses kidney calcium oxalate crystal depositions via reversing hyperoxaluria-induced oxidative stress damage. J Nanobiotechnology 20:516 (2022). PubMed: 36482378
View all Publications for this product

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1-3 of 3 Abreviews

Western blot abreview for Anti-Catalase antibody [EPR20198] - Peroxisome Marker

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Balb/C mice brain)
Gel Running Conditions
Reduced Denaturing (4-20% tris-glycin gel)
Loading amount
40 µg
Specification
Balb/C mice brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 11 2021

Western blot abreview for Anti-Catalase antibody [EPR20198] - Peroxisome Marker

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (SHSY5Y cells)
Gel Running Conditions
Reduced Denaturing (4-20% tris-glycin gel)
Loading amount
40 µg
Specification
SHSY5Y cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 11 2021

Immunohistochemistry (Frozen sections) abreview for Anti-Catalase antibody [EPR20198] - Peroxisome Marker

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (skin)
Permeabilization
No
Specification
skin
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Methanol
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. ALAIN BOUCAUD

Verified customer

Submitted Dec 19 2019

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