Anti-Catalase antibody - Peroxisome Marker (ab52477)
Key features and details
- Rabbit polyclonal to Catalase - Peroxisome Marker
- Suitable for: IP, ICC, WB, IHC-P
- Knockout validated
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-Catalase antibody - Peroxisome Marker
See all Catalase primary antibodies -
Description
Rabbit polyclonal to Catalase - Peroxisome Marker -
Host species
Rabbit -
Specificity
This antibody reacts with Catalase.
-
Tested applications
Suitable for: IP, ICC, WB, IHC-Pmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human Catalase aa 50-150 (N terminal).
(Peptide available asab215573) -
Positive control
- WB: TF1, Rat liver tissue, WT HAP1, HeLa, Jurkat, U251, U2OS, 9L, PC12 and RIE-1 whole cell lysates. IHC-P: Human liver tissue section.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab52477 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at an assay dependent concentration.
|
|
| ICC |
Use at an assay dependent concentration.
|
|
| WB | (8) |
1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
|
| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| Notes |
|---|
|
IP
Use at an assay dependent concentration. |
|
ICC
Use at an assay dependent concentration. |
|
WB
1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa). |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
-
Function
Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells. -
Involvement in disease
Defects in CAT are the cause of acatalasia (ACATLAS) [MIM:115500]; also known as acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions. -
Sequence similarities
Belongs to the catalase family. -
Post-translational
modificationsThe N-terminus is blocked. -
Cellular localization
Peroxisome. - Information by UniProt
-
Database links
- Entrez Gene: 847 Human
- Entrez Gene: 12359 Mouse
- Entrez Gene: 24248 Rat
- Omim: 115500 Human
- SwissProt: P04040 Human
- SwissProt: P24270 Mouse
- SwissProt: P04762 Rat
- Unigene: 502302 Human
see all -
Alternative names
- Cas1 antibody
- CAT antibody
- CATA_HUMAN antibody
see all
Images
-
Western blot - Anti-Catalase antibody - Peroxisome Marker (ab52477)Anti-Catalase antibody - Peroxisome Marker (ab52477) at 1/1000 dilution + TF1 lysate at 10 µg
Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa -
Western blot - Anti-Catalase antibody - Peroxisome Marker (ab52477)Anti-Catalase antibody - Peroxisome Marker (ab52477) at 1 µg/ml + Rat liver tissue lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa
Exposure time: 30 secondsGel type: MOPS
Blocking buffer: 2% BSA
-
Western blot - Anti-Catalase antibody - Peroxisome Marker (ab52477)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Catalase knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab52477 observed at 60 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab52477 was shown to specifically react with Catalase when Catalase knockout samples were used. Wild-type and Catalase knockout samples were subjected to SDS-PAGE. ab52477 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) andGoat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Catalase antibody - Peroxisome Marker (ab52477)IHC image of Catalasestaining in a formalin fixed, paraffin embedded normal human liver tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab52477, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
-
Western blot - Anti-Catalase antibody - Peroxisome Marker (ab52477)Image courtesy of an anonymous Abreview.All lanes : Anti-Catalase antibody - Peroxisome Marker (ab52477) at 1 µg/ml
Lane 1 : Whole cell lysate prepared from 293T cells
Lane 2 : Whole cell lysate prepared from HeLa cells
Lane 3 : Whole cell lysate prepared from U251 cells
Lane 4 : Whole cell lysate prepared from Jurkat cells
Lane 5 : Whole cell lysate prepared from U2OS cells
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP conjugated pig anti-rabbit polyclonal at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 60 kDa
Observed band size: 60 kDa
Exposure time: 5 minutes
-
Western blot - Anti-Catalase antibody - Peroxisome Marker (ab52477)Image courtesy of an anonymous Abreview.All lanes : Anti-Catalase antibody - Peroxisome Marker (ab52477) at 1 µg/ml
Lane 1 : Whole cell lysate prepared from 9L cells
Lane 2 : Whole cell lysate prepared from PC12 cells
Lane 3 : Whole cell lysate prepared from RIE-1 cells
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP conjugated pig anti-rabbit polyclonal at 1/5000 dilution
Developed using the ECL technique.
Predicted band size: 60 kDa
Observed band size: 60 kDa
Exposure time: 5 minutes
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (36)
ab52477 has been referenced in 36 publications.
- Matthies M et al. Nitric oxide (NO) synthase but not NO, HNO or H2 O2 mediates endothelium-dependent relaxation of resistance arteries from patients with cardiovascular disease. Br J Pharmacol 179:1049-1064 (2022). PubMed: 34664280
- Zhou Q et al. Ubiquitin-specific protease 3 attenuates interleukin-1β-mediated chondrocyte senescence by deacetylating forkhead box O-3 via sirtuin-3. Bioengineered 13:2017-2027 (2022). PubMed: 34847835
- Joseph LC et al. Combined metabolomic and transcriptomic profiling approaches reveal the cardiac response to high-fat diet. iScience 25:104184 (2022). PubMed: 35494220
- Deprez A et al. Transient neonatal exposure to hyperoxia, an experimental model of preterm birth, leads to skeletal muscle atrophy and fiber type switching. Clin Sci (Lond) 135:2589-2605 (2021). PubMed: 34750633
- Santiago Santana JM et al. Oxidative stress and neuroinflammation in a rat model of co-morbid obesity and psychogenic stress. Behav Brain Res 400:112995 (2021). PubMed: 33301815