Recombinant Anti-Cathepsin B antibody [EPR21033] (ab214428)
![Western blot - Anti-Cathepsin B antibody [EPR21033] (ab214428)](https://www.abcam.com/ps/products/214/ab214428/Images/ab214428-290953-anti-cathepsin-b-antibody-epr21033-western-blot.jpg "Western blot - Anti-Cathepsin B antibody [EPR21033] (ab214428)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21033] to Cathepsin B
- Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-Cathepsin B antibody [EPR21033]
See all Cathepsin B primary antibodies -
Description
Rabbit monoclonal [EPR21033] to Cathepsin B -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse kidney tissue lysate; Rat thymus and kidney tissue lysates; RAW 264.7 and PC-12 whole cell lysates. IHC-P: Mouse and rat kidney tissues. ICC/IF: RAW 264.7 and PC-12 cells. Flow Cyt (intra): RAW 264.7 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21033 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab214428 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/500.
|
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| IHC-P | (2) |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| WB | (2) |
1/1000. Predicted molecular weight: 37 kDa.
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| ICC/IF | (1) |
1/250.
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| Notes |
|---|
|
Flow Cyt (Intra)
1/500. |
|
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
WB
1/1000. Predicted molecular weight: 37 kDa. |
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ICC/IF
1/250. |
Target
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Function
Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis. -
Sequence similarities
Belongs to the peptidase C1 family. -
Cellular localization
Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 13030 Mouse
- Entrez Gene: 64529 Rat
- SwissProt: P10605 Mouse
- SwissProt: P00787 Rat
- Unigene: 236553 Mouse
- Unigene: 472420 Mouse
- Unigene: 100909 Rat
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Alternative names
- Amyloid precursor protein secretase antibody
- APP secretase antibody
- APPS antibody
see all
Images
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Western blot - Anti-Cathepsin B antibody [EPR21033] (ab214428)All lanes : Anti-Cathepsin B antibody [EPR21033] (ab214428) at 1/1000 dilution
Lane 1 : Mouse kidney tissue lysate
Lane 2 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : Rat thymus tissue lysate
Lane 4 : Rat kidney tissue lysate
Lane 5 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 37 kDa
Observed band size: 25,31,43 kDa why is the actual band size different from the predicted?Exposure times: Lanes 1,2: 23 seconds; Lane 3: 50 seconds; Lane 4: 15 seconds; Lane 5: 2 minutes.
Blocking/Dilution buffer: 5% NFDM/TBST.
The expression profile and molecular mass is consistent with what has been described in the literature (PMID:20536394; PMID: 10447678).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR21033] (ab214428)](https://www.abcam.com/ps/products/214/ab214428/Images/ab214428-290955-anti-cathepsin-b-antibody-epr21033-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR21033] (ab214428)Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Cathepsin B with ab214428 at 1/1000 dilution, followed by secondary Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Punctate cytoplasmic staining on mouse kidney (PMID: 20668705; PMID: 26831567) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR21033] (ab214428)](https://www.abcam.com/ps/products/214/ab214428/Images/ab214428-290956-anti-cathepsin-b-antibody-epr21033-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin B antibody [EPR21033] (ab214428)Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Cathepsin B with ab214428 at 1/1000 dilution, followed by secondary Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Punctate cytoplasmic staining on rat kidney (PMID: 20668705; PMID: 26831567) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin B antibody [EPR21033] (ab214428)](https://www.abcam.com/ps/products/214/ab214428/Images/ab214428-290957-anti-cathepsin-b-antibody-epr21033-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin B antibody [EPR21033] (ab214428)Immunofluorescent analysis of 100% methanol-fixed RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labeling Cathepsin B with ab214428 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in RAW 264.7 cells.
The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin B antibody [EPR21033] (ab214428)](https://www.abcam.com/ps/products/214/ab214428/Images/ab214428-290958-anti-cathepsin-b-antibody-epr21033-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin B antibody [EPR21033] (ab214428)Immunofluorescent analysis of 100% methanol-fixed PC-12 (rat adrenal gland pheochromocytoma cell line) cells labeling Cathepsin B with ab214428 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on PC-12 cells.
The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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![Flow Cytometry (Intracellular) - Anti-Cathepsin B antibody [EPR21033] (ab214428)](https://www.abcam.com/ps/products/214/ab214428/Images/ab214428-290959-anti-cathepsin-b-antibody-epr21033-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Cathepsin B antibody [EPR21033] (ab214428)Intracellular flow cytometric analysis of4% paraformaldehyde-fixed, 90% methanol-permeabilized RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) cell line labeling Cathepsin B with ab214428 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.
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![Anti-Cathepsin B antibody [EPR21033] (ab214428)](https://www.abcam.com/ps/products/214/ab214428/Images/ab214428-7-benefits-of-recombinant-antibodies.png)
Anti-Cathepsin B antibody [EPR21033] (ab214428)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (6)
ab214428 has been referenced in 6 publications.
- Zhang L et al. Ethionine Suppresses Mitochondria Autophagy and Induces Apoptosis via Activation of Reactive Oxygen Species in Neural Tube Defects. Front Neurol 11:242 (2020). PubMed: 32318018
- Tan CF et al. Exploring Extracellular Vesicles Biogenesis in Hypothalamic Cells through a Heavy Isotope Pulse/Trace Proteomic Approach. Cells 9:N/A (2020). PubMed: 32466345
- Tao Y et al. Autophagic-CTSB-inflammasome axis modulates hepatic stellate cells activation in arsenic-induced liver fibrosis. Chemosphere 242:124959 (2020). PubMed: 31669990
- Song Z et al. Comprehensive Proteomic Profiling of Urinary Exosomes and Identification of Potential Non-invasive Early Biomarkers of Alzheimer's Disease in 5XFAD Mouse Model. Front Genet 11:565479 (2020). PubMed: 33250918
- Sudhakar JN et al. Lumenal Galectin-9-Lamp2 interaction regulates lysosome and autophagy to prevent pathogenesis in the intestine and pancreas. Nat Commun 11:4286 (2020). PubMed: 32855403
- Zhang X et al. Ischemia-induced upregulation of autophagy preludes dysfunctional lysosomal storage and associated synaptic impairments in neurons. Autophagy N/A:1-24 (2020). PubMed: 33111641