Anti-Cathepsin K antibody (ab188604)
Key features and details
- Rabbit polyclonal to Cathepsin K
- Suitable for: IP, WB, IHC-P
- Reacts with: Mouse, Rat, Rabbit, Chicken, Human, Pig
- Isotype: IgG
Overview
-
Product name
Anti-Cathepsin K antibody
See all Cathepsin K primary antibodies -
Description
Rabbit polyclonal to Cathepsin K -
Host species
Rabbit -
Specificity
ab188604 detects proform (43 kDa) and the mature form (29 kDa) of Cathepsin K. -
Tested applications
Suitable for: IP, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Rabbit, Chicken, Human, Pig -
Immunogen
Synthetic peptide corresponding to Rat Cathepsin K. (Surrounding amino acid 321).
Database link: O35186 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 69% PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab188604 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
Use at an assay dependent concentration.
|
|
| WB |
Use at an assay dependent concentration.
|
|
| IHC-P |
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
IP
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. |
|
IHC-P
Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation. -
Tissue specificity
Predominantly expressed in osteclasts (bones). -
Involvement in disease
Defects in CTSK are the cause of pycnodysostosis (PKND) [MIM:265800]. PKND is an autosomal recessive osteochondrodysplasia characterized by osteosclerosis and short stature. -
Sequence similarities
Belongs to the peptidase C1 family. -
Cellular localization
Lysosome. - Information by UniProt
-
Database links
- Entrez Gene: 1513 Human
- Entrez Gene: 13038 Mouse
- Entrez Gene: 397569 Pig
- Entrez Gene: 100009334 Rabbit
- Entrez Gene: 29175 Rat
- Omim: 601105 Human
- SwissProt: P43235 Human
- SwissProt: P55097 Mouse
see all -
Alternative names
- Cathepsin K antibody
- Cathepsin O antibody
- Cathepsin O1 antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody (ab188604)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human thyroid tissue labeling Cathepsin K with ab188604 at 5 μg/ml. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Datasheets and documents
-
SDS download
-
Datasheet download
References (2)
ab188604 has been referenced in 2 publications.
- Sarkar J et al. Epigallocatechin-3-gallate inhibits osteoclastic differentiation by modulating mitophagy and mitochondrial functions. Cell Death Dis 13:908 (2022). PubMed: 36307395
- Kanji S et al. Dental pulp-derived stem cells inhibit osteoclast differentiation by secreting osteoprotegerin and deactivating AKT signalling in myeloid cells. J Cell Mol Med 25:2390-2403 (2021). PubMed: 33511706