Anti-Cathepsin K antibody (ab231590)
Key features and details
- Rabbit polyclonal to Cathepsin K
- Suitable for: IHC-P, WB
- Reacts with: Human, Pig
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Cathepsin K antibody
See all Cathepsin K primary antibodies -
Description
Rabbit polyclonal to Cathepsin K -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human, Pig
Predicted to work with: Cynomolgus monkey, Rhesus monkey -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human Cathepsin K aa 50 to the C-terminus. Expressed in E.coli.
Database link: P43235 -
Positive control
- WB: Recombinant human CTSK protein; pig heart tissue. IHC-P: Human breast cancer, prostate cancer, liver and lung cancer tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231590 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 37 kDa.
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Notes |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 37 kDa. |
Target
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Function
Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation. -
Tissue specificity
Predominantly expressed in osteclasts (bones). -
Involvement in disease
Defects in CTSK are the cause of pycnodysostosis (PKND) [MIM:265800]. PKND is an autosomal recessive osteochondrodysplasia characterized by osteosclerosis and short stature. -
Sequence similarities
Belongs to the peptidase C1 family. -
Cellular localization
Lysosome. - Information by UniProt
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Database links
- Entrez Gene: 102120416 Cynomolgus monkey
- Entrez Gene: 1513 Human
- Entrez Gene: 397569 Pig
- Entrez Gene: 574112 Rhesus monkey
- Omim: 601105 Human
- SwissProt: P61276 Cynomolgus monkey
- SwissProt: P43235 Human
- SwissProt: Q9GLE3 Pig
see all -
Alternative names
- Cathepsin K antibody
- Cathepsin O antibody
- Cathepsin O1 antibody
see all
Images
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Anti-Cathepsin K antibody (ab231590) at 3 µg/ml + Recombinant human Cathepsin K protein
Predicted band size: 37 kDa -
Anti-Cathepsin K antibody (ab231590) at 3 µg/ml + Pig heart tissue
Secondary
HRP-linked guinea pig anti-rabbit at 1/2000 dilution
Predicted band size: 37 kDa -
Formalin-fixed, paraffin-embedded human breast cancer tissue stained for Cathepsin K using ab231590 at 20 µg/mL in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human prostate cancer tissue stained for Cathepsin K using ab231590 at 20 µg/mL in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human liver tissue stained for Cathepsin K using ab231590 at 20 µg/mL in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human lung cancer tissue stained for Cathepsin K using ab231590 at 20 µg/mL in immunohistochemical analysis. DAB staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab231590 has not yet been referenced specifically in any publications.