Recombinant Anti-Cathepsin K antibody [EP9403] (ab187647)
![Western blot - Anti-Cathepsin K antibody [EP9403] (ab187647)](https://www.abcam.com/ps/products/187/ab187647/Images/ab187647-344767-Anti-cathepsin-k-antibody-EP9403-westernblot-mouse-rat.jpg "Western blot - Anti-Cathepsin K antibody [EP9403] (ab187647)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP9403] to Cathepsin K
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Cathepsin K antibody [EP9403]
See all Cathepsin K primary antibodies -
Description
Rabbit monoclonal [EP9403] to Cathepsin K -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human and rat cartilage lysate, mouse and rat muscle lysates, human skeletal muscle lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP9403 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187647 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 39 kDa (predicted molecular weight: 37 kDa).
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 39 kDa (predicted molecular weight: 37 kDa). |
Target
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Function
Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation. -
Tissue specificity
Predominantly expressed in osteclasts (bones). -
Involvement in disease
Defects in CTSK are the cause of pycnodysostosis (PKND) [MIM:265800]. PKND is an autosomal recessive osteochondrodysplasia characterized by osteosclerosis and short stature. -
Sequence similarities
Belongs to the peptidase C1 family. -
Cellular localization
Lysosome. - Information by UniProt
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Database links
- Entrez Gene: 1513 Human
- Entrez Gene: 13038 Mouse
- Entrez Gene: 29175 Rat
- Omim: 601105 Human
- SwissProt: P43235 Human
- SwissProt: P55097 Mouse
- SwissProt: O35186 Rat
- Unigene: 632466 Human
see all -
Alternative names
- Cathepsin K antibody
- Cathepsin O antibody
- Cathepsin O1 antibody
see all
Images
-
Western blot - Anti-Cathepsin K antibody [EP9403] (ab187647)All lanes : Anti-Cathepsin K antibody [EP9403] (ab187647) at 1/1000 dilution (purified)
Lane 1 : Mouse cartilage lysates
Lane 2 : Rat muscle lysates
Lane 3 : Mouse muscle lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted? -
![Western blot - Anti-Cathepsin K antibody [EP9403] (ab187647)](https://www.abcam.com/ps/products/187/ab187647/Images/ab187647-344771-Anti-cathepsin-k-antibody-EP9403-westernblot-human.jpg)
Western blot - Anti-Cathepsin K antibody [EP9403] (ab187647)All lanes : Anti-Cathepsin K antibody [EP9403] (ab187647) at 1/1000 dilution (Purified)
Lane 1 : Human cartilage lysates
Lane 2 : Human skeletal muscle lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 37 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted? -
![Western blot - Anti-Cathepsin K antibody [EP9403] (ab187647)](https://www.abcam.com/ps/products/187/ab187647/Images/ab187647-220681-anti-cathepsin-k-antibody-ep9403-western-blot.jpg)
Western blot - Anti-Cathepsin K antibody [EP9403] (ab187647)Anti-Cathepsin K antibody [EP9403] (ab187647) at 1/5000 dilution (unpurified) + Rat cartilage lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 37 kDa
Additional bands at: 39 kDa. We are unsure as to the identity of these extra bands. -
![Western blot - Anti-Cathepsin K antibody [EP9403] (ab187647)](https://www.abcam.com/ps/products/187/ab187647/Images/ab187647-220679-anti-cathepsin-k-antibody-ep9403-western-blot.jpg)
Western blot - Anti-Cathepsin K antibody [EP9403] (ab187647)Anti-Cathepsin K antibody [EP9403] (ab187647) at 1/1000 dilution (unpurified) + Mouse muscle lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 37 kDa
Additional bands at: 39 kDa. We are unsure as to the identity of these extra bands.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (8)
ab187647 has been referenced in 8 publications.
- Yang B et al. γ-Secretase inhibitors suppress IL-20-mediated osteoclastogenesis via Notch signalling and are affected by Notch2 in vitro. Scand J Immunol 96:e13169 (2022). PubMed: 35384009
- Cai P et al. Carnosol inhibits osteoclastogenesis in vivo and in vitro by blocking the RANKL‑induced NF‑κB signaling pathway. Mol Med Rep 26:N/A (2022). PubMed: 35593299
- Rossi F et al. Alteration of osteoclast activity in childhood cancer survivors: Role of iron and of CB2/TRPV1 receptors. PLoS One 17:e0271730 (2022). PubMed: 35862357
- Jiang C et al. Oxymatrine Attenuates Osteoclastogenesis via Modulation of ROS-Mediated SREBP2 Signaling and Counteracts Ovariectomy-Induced Osteoporosis. Front Cell Dev Biol 9:684007 (2021). PubMed: 34136493
- Yajun W et al. Betaine Attenuates Osteoarthritis by Inhibiting Osteoclastogenesis and Angiogenesis in Subchondral Bone. Front Pharmacol 12:723988 (2021). PubMed: 34658862
- Yan K et al. A20 inhibits osteoclastogenesis via TRAF6-dependent autophagy in human periodontal ligament cells under hypoxia. Cell Prolif 53:e12778 (2020). PubMed: 32027437
- Wang R et al. Synergistic effects of miR-708-5p and miR-708-3p accelerate the progression of osteoporosis. J Int Med Res 48:300060520978015 (2020). PubMed: 33322976
- Liu X et al. Upregulation of interleukin-8 and activin A induces osteoclastogenesis in ameloblastoma. Int J Mol Med 43:2329-2340 (2019). PubMed: 31017256