Recombinant Anti-Cathepsin K antibody [EPR24829-101] (BSA and Azide free) (ab300570)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (BSA and Azide free) (AB300570)](https://www.abcam.com/ps/products/300/ab300570/Images/AB300570-6-anti-cathepsin-k-antibody-epr24829101-immunohistochemistry-mouse-kidney.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (BSA and Azide free) (AB300570)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24829-101] to Cathepsin K - BSA and Azide free
- Suitable for: IHC-P, IHC-Fr
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-Cathepsin K antibody [EPR24829-101] (BSA and Azide free)
See all Cathepsin K primary antibodies -
Description
Rabbit monoclonal [EPR24829-101] to Cathepsin K - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IHC-Frmore details
Unsuitable for: Flow Cyt (Intra),ICC/IF,IP or WB -
Species reactivity
Reacts with: Mouse, Rat
Does not react with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Mouse bone, mouse kidney, rat bone, FFPE tissue sections. IHCFr: Rat P0 bone, aat liver fresh frozen tissues.
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General notes
ab300570 is the carrier-free version of ab300569.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24829-101 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab300570 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
| IHC-Fr |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
IHC-Fr
Use at an assay dependent concentration. |
Target
-
Function
Closely involved in osteoclastic bone resorption and may participate partially in the disorder of bone remodeling. Displays potent endoprotease activity against fibrinogen at acid pH. May play an important role in extracellular matrix degradation. -
Tissue specificity
Predominantly expressed in osteclasts (bones). -
Involvement in disease
Defects in CTSK are the cause of pycnodysostosis (PKND) [MIM:265800]. PKND is an autosomal recessive osteochondrodysplasia characterized by osteosclerosis and short stature. -
Sequence similarities
Belongs to the peptidase C1 family. -
Cellular localization
Lysosome. - Information by UniProt
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Database links
- Entrez Gene: 13038 Mouse
- Entrez Gene: 29175 Rat
- SwissProt: P55097 Mouse
- SwissProt: O35186 Rat
- Unigene: 272085 Mouse
- Unigene: 5598 Rat
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Alternative names
- Cathepsin K antibody
- Cathepsin O antibody
- Cathepsin O1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (BSA and Azide free) (AB300570)
This data was developed using ab300569, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Cathepsin K with ab300569 at 1/1000 dilution (0.529 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Negative control: no staining on mouse kidney (PMID:8647859). The section was incubated with ab300569 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (BSA and Azide free) (AB300570)](https://www.abcam.com/ps/products/300/ab300570/Images/AB300570-5-anti-cathepsin-k-antibody-epr24829101-immunohistochemistry-rat-bone.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (BSA and Azide free) (AB300570)This data was developed using ab300569, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded rat bone tissue labeling Cathepsin K with ab300569 at 1/1000 dilution (0.529 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Positive staining on osteoclasts in rat bone (PMID:29322325). The section was incubated with ab300569 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (BSA and Azide free) (AB300570)](https://www.abcam.com/ps/products/300/ab300570/Images/AB300570-4-anti-cathepsin-k-antibody-epr24829101-immunohistochemistry-mouse-bone.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin K antibody [EPR24829-101] (BSA and Azide free) (AB300570)This data was developed using ab300569, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse bone tissue labeling Cathepsin K with ab300569 at 1/1000 dilution (0.529 µg/mL) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection kit). Positive staining on osteoclasts in mouse bone (PMID:29322325). The section was incubated with ab300569 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: PBS was used instead of primary antibody, followed by a ready to use secondary antibody LeicaDS9800 (Bond™ Polymer Refine Detection kit).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
-
![Immunohistochemistry (Frozen sections) - Anti-Cathepsin K antibody [EPR24829-101] (BSA and Azide free) (AB300570)](https://www.abcam.com/ps/products/300/ab300570/Images/AB300570-3-anti-cathepsin-k-antibody-epr24829101-immunohistochemistry-frozen-rat-liver.jpg)
Immunohistochemistry (Frozen sections) - Anti-Cathepsin K antibody [EPR24829-101] (BSA and Azide free) (AB300570)This data was developed using ab300569, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver (fresh) tissue labeling Cathepsin K with ab300569 at 1/100 (5.29 µg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/ml) dilution (Green). Negative control: no staining on rat liver (PMID:25356585). is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 µg/ml) dilution.
-
![Immunohistochemistry (Frozen sections) - Anti-Cathepsin K antibody [EPR24829-101] (BSA and Azide free) (AB300570)](https://www.abcam.com/ps/products/300/ab300570/Images/AB300570-2-anti-cathepsin-k-antibody-epr24829101-immunohistochemistry-frozen-rat-p0-bone.jpg)
Immunohistochemistry (Frozen sections) - Anti-Cathepsin K antibody [EPR24829-101] (BSA and Azide free) (AB300570)This data was developed using ab300569, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat P0 bone (fresh) tissue labeling Cathepsin K with ab300569 at 1/100 (5.29 µg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/ml) dilution (Green). Positive staining on osteoclasts of rat P0 bone is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 µg/ml) dilution.
-
![Anti-Cathepsin K antibody [EPR24829-101] (BSA and Azide free) (AB300570)](https://www.abcam.com/ps/products/300/ab300570/Images/AB300570-1-Benefits-of-Recombinant-antibodies.jpg)
Anti-Cathepsin K antibody [EPR24829-101] (BSA and Azide free) (AB300570)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab300570 has not yet been referenced specifically in any publications.
