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RecombinantRabMAb

Recombinant Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)

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Reviews (6) Submit a question References (14)

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ELISA - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
  • Flow Cytometry (Intracellular) - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
  • Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
  • Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
  • ELISA - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
  • Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
  • Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
  • Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
  • Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
  • Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
  • OI-RD Scanning - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
  • Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8011] to Cathepsin L/V/K/H
  • Suitable for: ICC/IF, WB, IHC-P, Flow Cyt (Intra), ELISA
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 594 APC Carrier Free HRP PE

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View more associated products

Overview

  • Product name

    Anti-Cathepsin L/V/K/H antibody [EPR8011]
    See all Cathepsin L/V/K/H primary antibodies
  • Description

    Rabbit monoclonal [EPR8011] to Cathepsin L/V/K/H
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, Flow Cyt (Intra), ELISAmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Cathepsin L/V/K/H aa 100-200. The exact sequence is proprietary.
    Database link: P07711

  • Positive control

    • WB: A549, HCT-116, NIH/3T3, PC-12 and HepG2 whole cell lysate (ab7900). IHC-P: Human kidney tissue. ICC/IF: HepG2 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 4.40 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR8011
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Adhesion Proteins
    • Membrane Proteins
    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Amyloid
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Enzymes
    • Other Enzymes
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Alexa Fluor® 594 Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab208073)
    • Anti-Cathepsin L/V/K/H antibody [EPR8011] - BSA and Azide free (ab248589)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • NIH/3T3 whole cell lysate (ab7179)
    • A549 whole cell lysate (ab7910)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab133641 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (2)
1/100.
WB (3)
1/10000. Predicted molecular weight: 38 kDa.

For unpurified use at 1/1000 - 1/10000.

IHC-P (1)
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified use at 1/50 - 1/100.

Flow Cyt (Intra)
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
Notes
ICC/IF
1/100.
WB
1/10000. Predicted molecular weight: 38 kDa.

For unpurified use at 1/1000 - 1/10000.

IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

For unpurified use at 1/50 - 1/100.

Flow Cyt (Intra)
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.

Images

  • ELISA - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    ELISA - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)

    Antigen: CATL_HUMAN, CATV_HUMAN, CATS_HUMAN, CATK_HUMAN, CATH_HUMAN

    Antigen concentration: 1000ng/ml

    Primary antibody concentration range: 0~1000ng/ml

    Secondary antibody: Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

    Secondary antibody concentration: 1/2500

  • Flow Cytometry (Intracellular) - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    Flow Cytometry (Intracellular) - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)

    ab133641 staining Cathepsin Lin the human cell line HepG2 (human hepatocellular carcinoma) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/20. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody. 

    Isoytype control: Rabbit monoclonal IgG (Black) 

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue) 

     

     

  • Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    Lane 1 : Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641) at 1/20 dilution
    Lane 2 : Anti-Cathepsin L + V antibody [33/2] (ab6314) at 1/100 dilution
    Lane 3 : Anti-His tag at 1/10000 dilution

    All lanes : Human Cathepsin L recombinant protein fraction

    Secondary
    Lanes 1 & 3 : Peroxidase conjugated Goat Anti-Rabbit IgG, (H+L) at 1/1000 dilution
    Lane 2 : Rabbit anti-Mouse IgG at 1/2000 dilution

    Predicted band size: 38 kDa



    Blocking buffer and concentration:5% NFDM/TBST

    Diluting buffer and concentration:5% NFDM/TBST

    Observed MW:30

    Exposure time:3 minutes

  • Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    Lane 1 : Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641) at 1/20 dilution
    Lane 2 : Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641) at 1/100 dilution
    Lane 3 : Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641) at 1/500 dilution
    Lane 4 : Anti-Cathepsin L + V antibody [33/2] (ab6314) at 1/100 dilution
    Lane 5 : Anti-Cathepsin L + V antibody [33/2] (ab6314) at 1/1000 dilution
    Lane 6 : Anti-His tag at 1/10000 dilution

    All lanes : Human Cathepsin V recombinant protein fraction

    Secondary
    Lanes 1-3 : Peroxidase conjugated Goat Anti-Rabbit IgG, (H+L) at 1/1000 dilution
    Lanes 4-5 : Rabbit Anti-Mouse secondary ab at 1/2000 dilution
    Lane 6 : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 38 kDa



    Blocking buffer and concentration:5% NFDM/TBST

    Diluting buffer and concentration:5% NFDM/TBST

    Observed MW:30

    Exposure time:3 minutes

  • ELISA - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    ELISA - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)

    Direct ELISA antigen dose-response curve using purified ab133641.Antigen concentration of 1000ng/mL.Alkaline Phosphatase conjugated AffiniPure goat anti-rabbit IgG(H+L)(1/2500) was used as the secondary antibody.

  • Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641) at 1/20000 dilution (purified) + HepG2 cell lysate at 20 µg

    Secondary
    Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 38 kDa
    Observed band size: 38 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    All lanes : Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641) at 1/10000 dilution (purified)

    Lane 1 : HCT-116 cell lysate
    Lane 2 : A549 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 38 kDa
    Observed band size: 38 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    All lanes : Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641) at 1/20000 dilution (purified)

    Lane 1 : NIH/3T3 cell lysate
    Lane 2 : PC-12 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 38 kDa
    Observed band size: 38 kDa



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    All lanes : Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641) at 1/1000 dilution (unpurified)

    Lane 1 : A549 cell lysate
    Lane 2 : HCT-116 cell lysate
    Lane 3 : NIH/3T3 cell lysate
    Lane 4 : PC-12 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 38 kDa

  • Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641) at 1/1000 dilution (unpurified) + HepG2 cell lysate at 10 µg

    Secondary
    HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 38 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Cathepsin L with purified ab133641 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Cathepsin L with unpurified ab133641 at a 1/50 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)

    Immunocytochemistry/Immunofluorescence analysis of HT-29 cells labelling Cathepsin L with purified ab133641 at 1/100. Cells were fixed with 100% methanol and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/500) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.

    Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

  • Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)This image is courtesy of an Abreview submitted by Alina Macovei

    Unpurified ab133641 staining Cathepsin L in Human HepaRG cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% milk for 30 minutes at room temperature. Samples were incubated with primary antibody (1/1000) for 30 minutes. An Alexa Fluor® 488-conjugated Donkey anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.

    See Abreview

  • OI-RD Scanning - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    OI-RD Scanning - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
    Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (14)

Publishing research using ab133641? Please let us know so that we can cite the reference in this datasheet.

ab133641 has been referenced in 14 publications.

  • Hirunsai M & Srikuea R Autophagy-lysosomal signaling responses to heat stress in tenotomy-induced rat skeletal muscle atrophy. Life Sci 275:119352 (2021). PubMed: 33771521
  • Kim HJ  et al. Crosstalk between HSPA5 arginylation and sequential ubiquitination leads to AKT degradation through autophagy flux. Autophagy 17:961-979 (2021). PubMed: 32164484
  • Zhong B  et al. Caspase-8 Induces Lysosome-Associated Cell Death in Cancer Cells. Mol Ther 28:1078-1091 (2020). PubMed: 32053770
  • Kwon I Protective effects of endurance exercise on skeletal muscle remodeling against doxorubicin-induced myotoxicity in mice. Phys Act Nutr 24:11-21 (2020). PubMed: 32698257
  • Kim SY  et al. Non-Thermal Plasma Induces Antileukemic Effect Through mTOR Ubiquitination. Cells 9:N/A (2020). PubMed: 32131492
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-6 of 6 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Cathepsin L/V/K/H antibody [EPR8011]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (salivary gland)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Leica ER2 high pH buffer #AR9640
Permeabilization
Yes - Tween-20
Specification
salivary gland
Blocking step
Leica PowerVision IHC/ISH Super Blocking solution (PV6122) as blocking agent for 40 minute(s) · Concentration: 100% · Temperature: 22°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jul 04 2022

Western blot abreview for Anti-Cathepsin L antibody [EPR8011]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
10 µg
Sample
Trypanosoma brucei
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 09 2013

Western blot abreview for Anti-Cathepsin L antibody [EPR8011]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - whole cell (HepaRG)
Specification
HepaRG
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 25 2013

Immunocytochemistry/ Immunofluorescence abreview for Anti-Cathepsin L antibody [EPR8011]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 1µg/mL · Temperature: RT°C
Sample
Human Cell (HepG2)
Specification
HepG2
Permeabilization
Yes - 0.1% Triton X-100
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 25 2013

Immunocytochemistry/ Immunofluorescence

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
Sample
Human Cell (HepaRG)
Specification
HepaRG
Permeabilization
Yes - 0.1% Triton x-100
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MRS. Alina Macovei

Verified customer

Submitted May 24 2013

Western blot abreview for Anti-Cathepsin L antibody [EPR8011]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HepaRG)
Loading amount
20 µg
Specification
HepaRG
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MRS. Alina Macovei

Verified customer

Submitted May 15 2013

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