Recombinant Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
![ELISA - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)](https://www.abcam.com/ps/products/133/ab133641/Images/ab133641-256560-anti-cathepsin-l-v-k-h-antibody-epr8011-elisa.jpg "ELISA - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8011] to Cathepsin L/V/K/H
- Suitable for: ICC/IF, WB, IHC-P, Flow Cyt (Intra), ELISA
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Cathepsin L/V/K/H antibody [EPR8011]
See all Cathepsin L/V/K/H primary antibodies -
Description
Rabbit monoclonal [EPR8011] to Cathepsin L/V/K/H -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-P, Flow Cyt (Intra), ELISAmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Cathepsin L/V/K/H aa 100-200. The exact sequence is proprietary.
Database link: P07711 -
Positive control
- WB: A549, HCT-116, NIH/3T3, PC-12 and HepG2 whole cell lysate (ab7900). IHC-P: Human kidney tissue. ICC/IF: HepG2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 4.40 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8011 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133641 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (2) |
1/100.
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| WB | (3) |
1/10000. Predicted molecular weight: 38 kDa.
For unpurified use at 1/1000 - 1/10000. |
| IHC-P | (1) |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
For unpurified use at 1/50 - 1/100. |
| Flow Cyt (Intra) |
Use at an assay dependent concentration.
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| ELISA |
Use at an assay dependent concentration.
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| Notes |
|---|
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ICC/IF
1/100. |
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WB
1/10000. Predicted molecular weight: 38 kDa. For unpurified use at 1/1000 - 1/10000. |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. For unpurified use at 1/50 - 1/100. |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
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ELISA
Use at an assay dependent concentration. |
Images
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ELISA - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
Antigen: CATL_HUMAN, CATV_HUMAN, CATS_HUMAN, CATK_HUMAN, CATH_HUMAN
Antigen concentration: 1000ng/ml
Primary antibody concentration range: 0~1000ng/ml
Secondary antibody: Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Secondary antibody concentration: 1/2500
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![Flow Cytometry (Intracellular) - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)](https://www.abcam.com/ps/products/133/ab133641/Images/ab133641-268979-anti-cathepsin-l-v-k-h-antibody-epr8011-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)ab133641 staining Cathepsin Lin the human cell line HepG2 (human hepatocellular carcinoma) by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/20. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black)
Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
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![Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)](https://www.abcam.com/ps/products/133/ab133641/Images/ab133641-256344-anti-cathepsin-l-v-k-h-antibody-epr8011-western-blot.jpg)
Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)Lane 1 : Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641) at 1/20 dilution
Lane 2 : Anti-Cathepsin L + V antibody [33/2] (ab6314) at 1/100 dilution
Lane 3 : Anti-His tag at 1/10000 dilution
All lanes : Human Cathepsin L recombinant protein fraction
Secondary
Lanes 1 & 3 : Peroxidase conjugated Goat Anti-Rabbit IgG, (H+L) at 1/1000 dilution
Lane 2 : Rabbit anti-Mouse IgG at 1/2000 dilution
Predicted band size: 38 kDaBlocking buffer and concentration:5% NFDM/TBST
Diluting buffer and concentration:5% NFDM/TBST
Observed MW:30
Exposure time:3 minutes
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![Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)](https://www.abcam.com/ps/products/133/ab133641/Images/ab133641-256346-anti-cathepsin-l-v-k-h-antibody-epr8011-western-blot.jpg)
Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)Lane 1 : Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641) at 1/20 dilution
Lane 2 : Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641) at 1/100 dilution
Lane 3 : Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641) at 1/500 dilution
Lane 4 : Anti-Cathepsin L + V antibody [33/2] (ab6314) at 1/100 dilution
Lane 5 : Anti-Cathepsin L + V antibody [33/2] (ab6314) at 1/1000 dilution
Lane 6 : Anti-His tag at 1/10000 dilution
All lanes : Human Cathepsin V recombinant protein fraction
Secondary
Lanes 1-3 : Peroxidase conjugated Goat Anti-Rabbit IgG, (H+L) at 1/1000 dilution
Lanes 4-5 : Rabbit Anti-Mouse secondary ab at 1/2000 dilution
Lane 6 : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 38 kDaBlocking buffer and concentration:5% NFDM/TBST
Diluting buffer and concentration:5% NFDM/TBST
Observed MW:30
Exposure time:3 minutes
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![ELISA - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)](https://www.abcam.com/ps/products/133/ab133641/Images/ab133641-256348-anti-cathepsin-l-v-k-h-antibody-epr8011-elisa.jpg)
ELISA - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)Direct ELISA antigen dose-response curve using purified ab133641.Antigen concentration of 1000ng/mL.Alkaline Phosphatase conjugated AffiniPure goat anti-rabbit IgG(H+L)(1/2500) was used as the secondary antibody.
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![Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)](https://www.abcam.com/ps/products/133/ab133641/Images/ab133641-250235-anti-cathepsin-l-v-k-h-antibody-epr8011-western-blot.jpg)
Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641) at 1/20000 dilution (purified) + HepG2 cell lysate at 20 µg
Secondary
Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDaBlocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)](https://www.abcam.com/ps/products/133/ab133641/Images/ab133641-250236-anti-cathepsin-l-v-k-h-antibody-epr8011-western-blot.jpg)
Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)All lanes : Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641) at 1/10000 dilution (purified)
Lane 1 : HCT-116 cell lysate
Lane 2 : A549 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDaBlocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)](https://www.abcam.com/ps/products/133/ab133641/Images/ab133641-250237-anti-cathepsin-l-v-k-h-antibody-epr8011-western-blot.jpg)
Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)All lanes : Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641) at 1/20000 dilution (purified)
Lane 1 : NIH/3T3 cell lysate
Lane 2 : PC-12 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDaBlocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)](https://www.abcam.com/ps/products/133/ab133641/Images/ab133641-147566-anti-cathepsin-l-v-k-h-antibody-epr8011-western-blot.jpg)
Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)All lanes : Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641) at 1/1000 dilution (unpurified)
Lane 1 : A549 cell lysate
Lane 2 : HCT-116 cell lysate
Lane 3 : NIH/3T3 cell lysate
Lane 4 : PC-12 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 38 kDa -
![Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)](https://www.abcam.com/ps/products/133/ab133641/Images/ab133641-147565-anti-cathepsin-l-v-k-h-antibody-epr8011-western-blot.jpg)
Western blot - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641) at 1/1000 dilution (unpurified) + HepG2 cell lysate at 10 µg
Secondary
HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 38 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)](https://www.abcam.com/ps/products/133/ab133641/Images/ab133641-250258-anti-cathepsin-l-v-k-h-antibody-epr8011-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Cathepsin L with purified ab133641 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)](https://www.abcam.com/ps/products/133/ab133641/Images/ab133641-147564-anti-cathepsin-l-v-k-h-antibody-epr8011-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Cathepsin L with unpurified ab133641 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)](https://www.abcam.com/ps/products/133/ab133641/Images/ab133641-250268-anti-cathepsin-l-v-k-h-antibody-epr8011-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)Immunocytochemistry/Immunofluorescence analysis of HT-29 cells labelling Cathepsin L with purified ab133641 at 1/100. Cells were fixed with 100% methanol and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/500) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.
Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).
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![Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)](https://www.abcam.com/ps/products/133/ab133641/Images/ab133641-194855-anti-cathepsin-l-v-k-h-antibody-epr8011-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)This image is courtesy of an Abreview submitted by Alina MacoveiUnpurified ab133641 staining Cathepsin L in Human HepaRG cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% milk for 30 minutes at room temperature. Samples were incubated with primary antibody (1/1000) for 30 minutes. An Alexa Fluor® 488-conjugated Donkey anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.
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![OI-RD Scanning - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)](https://www.abcam.com/ps/products/133/ab133641/Images/ab133641-147563-anti-cathepsin-l-v-k-h-antibody-epr8011.jpg)
OI-RD Scanning - Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641) -
![Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)](https://www.abcam.com/ps/products/133/ab133641/Images/ab133641-16-benefits-of-recombinant-antibodies.png)
Anti-Cathepsin L/V/K/H antibody [EPR8011] (ab133641)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab133641 has been referenced in 15 publications.
- Brun S et al. GNS561, a clinical-stage PPT1 inhibitor, is efficient against hepatocellular carcinoma via modulation of lysosomal functions. Autophagy 18:678-694 (2022). PubMed: 34740311
- Hirunsai M & Srikuea R Autophagy-lysosomal signaling responses to heat stress in tenotomy-induced rat skeletal muscle atrophy. Life Sci 275:119352 (2021). PubMed: 33771521
- Kim HJ et al. Crosstalk between HSPA5 arginylation and sequential ubiquitination leads to AKT degradation through autophagy flux. Autophagy 17:961-979 (2021). PubMed: 32164484
- Zhong B et al. Caspase-8 Induces Lysosome-Associated Cell Death in Cancer Cells. Mol Ther 28:1078-1091 (2020). PubMed: 32053770
- Kwon I Protective effects of endurance exercise on skeletal muscle remodeling against doxorubicin-induced myotoxicity in mice. Phys Act Nutr 24:11-21 (2020). PubMed: 32698257