Anti-Caveolin-1 antibody [7C8] (ab17052)
![Western blot - Anti-Caveolin-1 antibody [7C8] (ab17052)](https://www.abcam.com/ps/products/17/ab17052/Images/ab17052-395835-anti-caveolin-1-antibody-7c8-western-blot-a431-wt-a431-cav1-knockout-a549-hela.jpg "Western blot - Anti-Caveolin-1 antibody [7C8] (ab17052)")
Key features and details
- Mouse monoclonal [7C8] to Caveolin-1
- Suitable for: WB, ICC
- Knockout validated
- Reacts with: Human
- Isotype: IgG2b
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Caveolin-1 antibody [7C8]
See all Caveolin-1 primary antibodies -
Description
Mouse monoclonal [7C8] to Caveolin-1 -
Host species
Mouse -
Specificity
The monoclonal antibody 7C8 recognizes caveolin-1a as well as caveolin-1ß, which are present in many tissues, like aorta, heart, muscle, lung, adipose white, brown and epidydimal fat. -
Tested applications
Suitable for: WB, ICCmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Rat Caveolin-1. GLUT4-containing vesicles immunoadsorbed from low density microsomes of rat adipocytes (Sprague Dawley). The antibody recognises epitope between residue 32 and the C-terminus.
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Positive control
- WB: A431, A549 and HUVEC cell lysates. ICC: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
0.2 µm filtered -
Clonality
Monoclonal -
Clone number
7C8 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab17052 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (3) |
Use at an assay dependent concentration. Detects a band of approximately 21, 19 kDa.
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| ICC |
Use at an assay dependent concentration.
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| Notes |
|---|
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WB
Use at an assay dependent concentration. Detects a band of approximately 21, 19 kDa. |
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ICC
Use at an assay dependent concentration. |
Target
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Function
May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway. -
Tissue specificity
Expressed in muscle and lung, less so in liver, brain and kidney. -
Involvement in disease
Defects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes. -
Sequence similarities
Belongs to the caveolin family. -
Post-translational
modificationsThe initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated. -
Cellular localization
Golgi apparatus membrane. Cell membrane. Membrane > caveola. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. - Information by UniProt
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Database links
- Entrez Gene: 857 Human
- Omim: 601047 Human
- SwissProt: Q03135 Human
- Unigene: 74034 Human
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Alternative names
- BSCL3 antibody
- CAV antibody
- CAV1 antibody
see all
Images
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Western blot - Anti-Caveolin-1 antibody [7C8] (ab17052)All lanes : Anti-Caveolin-1 antibody [7C8] (ab17052) at 1 µg/ml
Lane 1 : Wild-type A-431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 2 : CAV1 knockout A-431 (Human epidermoid carcinoma cell line) whole cell lysate
Lane 3 : A549 (Human lung carcinoma cell line) whole cell lysate
Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Observed band size: 21-24 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab17052 observed at 21-24 kDa. Red - loading control, ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55kDa.
ab17052 was shown to react with Caveolin-1 in wild-type A431 cells in western blot. Loss of signal was observed when CAV1 knockout sample was used. Wild-type and CAV1 knockout A431 cell lysates were subjected to SDS-PAGE. Membranes were blocked in non-mammalian (TBS-based) blocking solution before incubation with ab17052 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at 1 µg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry - Anti-Caveolin-1 antibody [7C8] (ab17052)](https://www.abcam.com/ps/products/17/ab17052/Images/ab17052-7-anti-caveolin-1-antibody-7c8-immunocytochemistry-hela-human.jpg)
Immunocytochemistry - Anti-Caveolin-1 antibody [7C8] (ab17052)ab17052 staining Caveolin-1 in wild-type HeLa cells (top panel) and CAV1 knockout HeLa cells (ab255371) (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab17052 at 1/500 dilution and ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8). -
![Immunocytochemistry - Anti-Caveolin-1 antibody [7C8] (ab17052)](https://www.abcam.com/ps/products/17/ab17052/Images/ab17052-25892-anti-caveolin-1-antibody-7c8-immunofluorescence.jpg)
Immunocytochemistry - Anti-Caveolin-1 antibody [7C8] (ab17052)This image is courtesy of an anonymous abreview.ab17052 staining Caveolin-1 in human Hacat keratinocyte cells by Immunocytochemistry/ Immunofluorescence. The cells were formaldehyde fixed, permeabilised in 0.1% Triton X-100 and then blocked using 1% serum for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/75 for 24 hours at 4°C. The secondary antibody used was conjugated to Alexa Fluor® 488 (green) used at a 1/500 dilution.
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![Western blot - Anti-Caveolin-1 antibody [7C8] (ab17052)](https://www.abcam.com/ps/products/17/ab17052/Images/ab17052-276490-anti-caveolin-1-antibody-7c8-western-blot.jpg)
Western blot - Anti-Caveolin-1 antibody [7C8] (ab17052)This image is courtesy of an anonymous Abreview.Anti-Caveolin-1 antibody [7C8] (ab17052) at 1/500 dilution + HUVEC whole cell lysate at 20 µg
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 10 seconds
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![Immunocytochemistry - Anti-Caveolin-1 antibody [7C8] (ab17052)](https://www.abcam.com/ps/products/17/ab17052/Images/ab17052-276492-anti-caveolin-1-antibody-7c8-immunofluorescence.jpg)
Immunocytochemistry - Anti-Caveolin-1 antibody [7C8] (ab17052)This image is courtesy of an anonymous Abreview.Immunocytochemical analysis of Mouse Bend.3 cells, labeling Caveolin-1 with ab17052. Cells were paraformaldehyde fixed, permeabilized with PBS + 0.1% Triton (PBT), and blocked with 10% serum for 1 hour at 22°C. Staining with ab17052 (diluted 1/200) was for 16 hours at 4°C.
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![Immunocytochemistry - Anti-Caveolin-1 antibody [7C8] (ab17052)](https://www.abcam.com/ps/products/17/ab17052/Images/ab17052-25893-anti-caveolin-1-antibody-7c8-immunofluorescence.jpg)
Immunocytochemistry - Anti-Caveolin-1 antibody [7C8] (ab17052)This image is courtesy of an anonymous Abreviewab17052 at a 1/500 dilution staining hamster CHO cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with 10% serum prior to incubation with the antibody. Bound antibody was detected using a FITC conjugated goat anti-mouse antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (35)
ab17052 has been referenced in 35 publications.
- Tian Y et al. Role of Caveolae in the Development of Microvascular Dysfunction and Hyperglycemia in Type 2 Diabetes. Front Physiol 13:825018 (2022). PubMed: 35250626
- Yeung CC et al. Circadian regulation of protein cargo in extracellular vesicles. Sci Adv 8:eabc9061 (2022). PubMed: 35394844
- Li A et al. Mechanical properties of tunneling nanotube and its mechanical stability in human embryonic kidney cells. Front Cell Dev Biol 10:955676 (2022). PubMed: 36238686
- Kearney AL et al. Akt phosphorylates insulin receptor substrate to limit PI3K-mediated PIP3 synthesis. Elife 10:N/A (2021). PubMed: 34253290
- van der Horst J et al. Dynein regulates Kv7.4 channel trafficking from the cell membrane. J Gen Physiol 153:N/A (2021). PubMed: 33533890