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    products/primary-antibodies/caveolin-1-antibody-7c8-ab17052.pdf

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Validated using a knockout cell line

Anti-Caveolin-1 antibody [7C8] (ab17052)

  • Datasheet
  • SDS
Reviews (13)Q&A (4)References (31)

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Western blot - Anti-Caveolin-1 antibody [7C8] (ab17052)
  • Immunocytochemistry - Anti-Caveolin-1 antibody [7C8] (ab17052)
  • Immunocytochemistry - Anti-Caveolin-1 antibody [7C8] (ab17052)
  • Western blot - Anti-Caveolin-1 antibody [7C8] (ab17052)
  • Immunocytochemistry - Anti-Caveolin-1 antibody [7C8] (ab17052)
  • Immunocytochemistry - Anti-Caveolin-1 antibody [7C8] (ab17052)

Key features and details

  • Mouse monoclonal [7C8] to Caveolin-1
  • Suitable for: WB, ICC
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG2b

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Caveolin-1 antibody [E249] - Caveolae Marker (ab32577)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Caveolin-1 antibody [7C8]
    See all Caveolin-1 primary antibodies
  • Description

    Mouse monoclonal [7C8] to Caveolin-1
  • Host species

    Mouse
  • Specificity

    The monoclonal antibody 7C8 recognizes caveolin-1a as well as caveolin-1ß, which are present in many tissues, like aorta, heart, muscle, lung, adipose white, brown and epidydimal fat.
  • Tested applications

    Suitable for: WB, ICCmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Rat Caveolin-1. GLUT4-containing vesicles immunoadsorbed from low density microsomes of rat adipocytes (Sprague Dawley). The antibody recognises epitope between residue 32 and the C-terminus.

  • Positive control

    • WB: A431, A549 and HUVEC cell lysates. ICC: HeLa cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: PBS, 0.1% BSA
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    0.2 µm filtered
  • Clonality

    Monoclonal
  • Clone number

    7C8
  • Isotype

    IgG2b
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cholesterol Metabolism
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Coat Proteins
    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Caveolae and Clathrin
    • Cancer
    • Tumor biomarkers
    • Other
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Cholesterol Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer
    • Neuroscience
    • Processes

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Isotype control

    • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)
  • KO cell lines

    • Human CAV1 (Caveolin-1) knockout HeLa cell line (ab255371)
    • Human CAV1 (Caveolin-1) knockout A-431 cell line (ab269583)
  • KO cell lysates

    • Human CAV1 (Caveolin-1) knockout A-431 cell lysate (ab270706)
  • Recombinant Protein

    • Recombinant Human Caveolin-1 protein (ab114170)
  • Related Products

    • Anti-beta Tubulin antibody - Loading Control (ab6046)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab17052 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (3)
Use at an assay dependent concentration. Detects a band of approximately 21, 19 kDa.
ICC
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent concentration. Detects a band of approximately 21, 19 kDa.
ICC
Use at an assay dependent concentration.
Application notes
Is unsuitable for Flow Cyt.

Target

  • Function

    May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway.
  • Tissue specificity

    Expressed in muscle and lung, less so in liver, brain and kidney.
  • Involvement in disease

    Defects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes.
  • Sequence similarities

    Belongs to the caveolin family.
  • Post-translational
    modifications

    The initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated.
  • Cellular localization

    Golgi apparatus membrane. Cell membrane. Membrane > caveola. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae.
  • Target information above from: UniProt accession Q03135 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 857 Human
    • Omim: 601047 Human
    • SwissProt: Q03135 Human
    • Unigene: 74034 Human
    • Alternative names

      • BSCL3 antibody
      • CAV antibody
      • CAV1 antibody
      • CAV1_HUMAN antibody
      • caveolae protein, 22 kD antibody
      • caveolin 1 alpha isoform antibody
      • caveolin 1 beta isoform antibody
      • Caveolin 1 caveolae protein 22kDa antibody
      • Caveolin-1 antibody
      • Caveolin1 antibody
      • cell growth-inhibiting protein 32 antibody
      • CGL3 antibody
      • LCCNS antibody
      • MSTP085 antibody
      • OTTHUMP00000025031 antibody
      • PPH3 antibody
      • VIP 21 antibody
      • VIP21 antibody
      see all

    Images

    • Western blot - Anti-Caveolin-1 antibody [7C8] (ab17052)
      Western blot - Anti-Caveolin-1 antibody [7C8] (ab17052)
      All lanes : Anti-Caveolin-1 antibody [7C8] (ab17052) at 1 µg/ml

      Lane 1 : Wild-type A-431 (Human epidermoid carcinoma cell line) whole cell lysate
      Lane 2 : CAV1 knockout A-431 (Human epidermoid carcinoma cell line) whole cell lysate
      Lane 3 : A549 (Human lung carcinoma cell line) whole cell lysate
      Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Observed band size: 21-24 kDa why is the actual band size different from the predicted?



      Lanes 1 - 4: Merged signal (red and green). Green - ab17052 observed at 21-24 kDa. Red - loading control, ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55kDa.

      ab17052 was shown to react with Caveolin-1 in wild-type A431 cells in western blot. Loss of signal was observed when CAV1 knockout sample was used. Wild-type and CAV1 knockout A431 cell lysates were subjected to SDS-PAGE. Membranes were blocked in non-mammalian (TBS-based) blocking solution before incubation with ab17052 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at 1 µg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Immunocytochemistry - Anti-Caveolin-1 antibody [7C8] (ab17052)
      Immunocytochemistry - Anti-Caveolin-1 antibody [7C8] (ab17052)

      ab17052 staining Caveolin-1 in wild-type HeLa cells (top panel) and CAV1 knockout HeLa cells (ab255371) (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab17052 at 1/500 dilution and ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
      Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

    • Immunocytochemistry - Anti-Caveolin-1 antibody [7C8] (ab17052)
      Immunocytochemistry - Anti-Caveolin-1 antibody [7C8] (ab17052)This image is courtesy of an anonymous abreview.

       ab17052 staining Caveolin-1 in human Hacat keratinocyte cells by Immunocytochemistry/ Immunofluorescence. The cells were formaldehyde fixed, permeabilised in 0.1% Triton X-100 and then blocked using 1% serum for 1 hour at 25°C. Samples were then incubated with primary antibody at 1/75 for 24 hours at 4°C. The secondary antibody used was conjugated to Alexa Fluor® 488 (green) used at a 1/500 dilution.

      See Abreview

    • Western blot - Anti-Caveolin-1 antibody [7C8] (ab17052)
      Western blot - Anti-Caveolin-1 antibody [7C8] (ab17052)This image is courtesy of an anonymous Abreview.
      Anti-Caveolin-1 antibody [7C8] (ab17052) at 1/500 dilution + HUVEC whole cell lysate at 20 µg

      Developed using the ECL technique.

      Performed under reducing conditions.

      Exposure time: 10 seconds

      See Abreview

    • Immunocytochemistry - Anti-Caveolin-1 antibody [7C8] (ab17052)
      Immunocytochemistry - Anti-Caveolin-1 antibody [7C8] (ab17052)This image is courtesy of an anonymous Abreview.

      Immunocytochemical analysis of Mouse Bend.3 cells, labeling Caveolin-1 with ab17052. Cells were paraformaldehyde fixed, permeabilized with PBS + 0.1% Triton (PBT), and blocked with 10% serum for 1 hour at 22°C. Staining with ab17052 (diluted 1/200) was for 16 hours at 4°C.

      See Abreview

    • Immunocytochemistry - Anti-Caveolin-1 antibody [7C8] (ab17052)
      Immunocytochemistry - Anti-Caveolin-1 antibody [7C8] (ab17052)This image is courtesy of an anonymous Abreview

      ab17052 at a 1/500 dilution staining hamster CHO cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with 10% serum prior to incubation with the antibody. Bound antibody was detected using a FITC conjugated goat anti-mouse antibody.

      See Abreview

    Protocols

    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (31)

    Publishing research using ab17052? Please let us know so that we can cite the reference in this datasheet.

    ab17052 has been referenced in 31 publications.

    • Li Y  et al. Dynamic Dissection of the Endocytosis of Porcine Epidemic Diarrhea Coronavirus Cooperatively Mediated by Clathrin and Caveolae as Visualized by Single-Virus Tracking. mBio 12:N/A (2021). PubMed: 33785615
    • Zhang W  et al. Moderate mechanical stimulation rescues degenerative annulus fibrosus by suppressing caveolin-1 mediated pro-inflammatory signaling pathway. Int J Biol Sci 17:1395-1412 (2021). PubMed: 33867854
    • Kearney AL  et al. Akt phosphorylates insulin receptor substrate to limit PI3K-mediated PIP3 synthesis. Elife 10:N/A (2021). PubMed: 34253290
    • Wang Q  et al. Caveolin-1 modulates hypertensive vascular remodeling via regulation of the Notch pathway. Mol Med Rep 22:4320-4328 (2020). PubMed: 33000233
    • Wang Y  et al. 27-Hydroxycholesterol Promotes the Transfer of Astrocyte-Derived Cholesterol to Neurons in Co-cultured SH-SY5Y Cells and C6 Cells. Front Cell Dev Biol 8:580599 (2020). PubMed: 33330456
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 17 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Caveolin-1 antibody [7C8]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Cardiac myocyte)
    Permeabilization
    Yes - Triton 0.1%
    Specification
    Cardiac myocyte
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 31 2017

    Western blot abreview for Anti-Caveolin-1 antibody [7C8]

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Bos taurus Cell lysate - other (HEK293T cells and BoMAC (bovine macrophages) Lyse)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    15 µg
    Specification
    HEK293T cells and BoMAC (bovine macrophages) Lyse
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 08 2016

    Western blot abreview for Anti-Caveolin-1 antibody [7C8]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (pericytes)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    20 µg
    Specification
    pericytes
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Feb 19 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Caveolin-1 antibody [7C8]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (pericytes)
    Permeabilization
    Yes - 0.1% Triton in PBS
    Specification
    pericytes
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 18 2015

    Western blot abreview for Anti-Caveolin-1 antibody [7C8]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HUVEC)
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Loading amount
    20 µg
    Specification
    HUVEC
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 07 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Caveolin-1 antibody [7C8]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HRMEC)
    Permeabilization
    Yes - 0.1% triton in PBS
    Specification
    HRMEC
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Nov 23 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Caveolin-1 antibody [7C8]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Pig Cell (PAEC)
    Specification
    PAEC
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Aug 27 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Caveolin-1 [7C8] antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
    Sample
    Mouse Cell (bEnd.3)
    Specification
    bEnd.3
    Permeabilization
    Yes - PBT (PBS+0.1%Triton)
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jul 17 2014

    Question

    Customer kindly contacted us as ab2910 was not giving specific signal in ICC/IF.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 21 2012

    Answer

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number xxxx. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

    Read More

    Abcam Scientific Support

    Answered on Nov 21 2012

    Question

    HeLa cells are stained uniformly instead of showing the correct localization.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 17 2012

    Answer

    Thank you very much for your call today and for letting us know about the trouble with ab17052.

    Please keep me updated about the results after trying the protocol alterations that we discussed. If the antibody still does not show the correct staining, I'll be happy to send a different antibody to try or alternatively issue a credit or refund.

    I look forward to hearing from you. If you have any questions or if there is anything else that we can do for you, please let me know and I'll be happy to help.

    Read More

    Abcam Scientific Support

    Answered on Oct 17 2012

    1-10 of 17 Abreviews or Q&A

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