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    products/primary-antibodies/caveolin-1-antibody-epr15554-n-terminal-ab192869.pdf

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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab192869)

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  • Certificate of Compliance
Reviews (2) Submit a question References (7)

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Western blot - Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab192869)
  • Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab192869)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab192869)
  • Flow Cytometry (Intracellular) - Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab192869)
  • Western blot - Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab192869)
  • Western blot - Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab192869)
  • Immunoprecipitation - Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab192869)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab192869)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab192869)
  • Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab192869)
  • Flow Cytometry (Intracellular) - Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab192869)
  • Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab192869)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15554] to Caveolin-1 - N-terminal
  • Suitable for: ICC/IF, Flow Cyt (Intra), WB, IHC-P, IP
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free PE

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Overview

  • Product name

    Anti-Caveolin-1 antibody [EPR15554] - N-terminal
    See all Caveolin-1 primary antibodies
  • Description

    Rabbit monoclonal [EPR15554] to Caveolin-1 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, Flow Cyt (Intra), WB, IHC-P, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: PC-3, A549, A431 and HeLa cell lysates. IHC-P: Human liver and squamous cell carcinoma of cervix tissues; mouse lung tissue. ICC/IF: A673 and HeLa cells. Flow Cyt (intra): NIH3T3 and HeLa cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15554
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cholesterol Metabolism
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Coat Proteins
    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Caveolae and Clathrin
    • Cancer
    • Tumor biomarkers
    • Other
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Cholesterol Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • PE Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab212007)
    • Alexa Fluor® 488 Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab225380)
    • Alexa Fluor® 647 Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab225381)
    • Anti-Caveolin-1 antibody [EPR15554] - BSA and Azide free (ab240332)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human CAV1 (Caveolin-1) knockout HeLa cell line (ab255371)
    • Human CAV1 (Caveolin-1) knockout A-431 cell line (ab269583)
  • KO cell lysates

    • Human CAV1 (Caveolin-1) knockout HeLa cell lysate (ab263806)
    • Human CAV1 (Caveolin-1) knockout A-431 cell lysate (ab270706)
  • Positive Controls

    • A-431 whole cell lysate (ab30132)
    • A-431 whole cell lysate (ab7909)
    • A549 whole cell lysate (ab7910)
  • Recombinant Protein

    • Recombinant Human Caveolin-1 protein (ab114170)
  • Related Products

    • Recombinant Human Caveolin-1 protein (ab114170)
    • Alexa Fluor® 488 Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab225380)
    • Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab192869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/300.
Flow Cyt (Intra)
1/120.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/10000 - 1/50000. Detects a band of approximately 17, 20 kDa (predicted molecular weight: 17, 20 kDa).
IHC-P (2)
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/30.
Notes
ICC/IF
1/300.
Flow Cyt (Intra)
1/120.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/10000 - 1/50000. Detects a band of approximately 17, 20 kDa (predicted molecular weight: 17, 20 kDa).
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
1/30.

Target

  • Function

    May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway.
  • Tissue specificity

    Expressed in muscle and lung, less so in liver, brain and kidney.
  • Involvement in disease

    Defects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes.
  • Sequence similarities

    Belongs to the caveolin family.
  • Post-translational
    modifications

    The initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated.
  • Cellular localization

    Golgi apparatus membrane. Cell membrane. Membrane > caveola. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae.
  • Target information above from: UniProt accession Q03135 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 857 Human
    • Entrez Gene: 12389 Mouse
    • Omim: 601047 Human
    • SwissProt: Q03135 Human
    • SwissProt: P49817 Mouse
    • Unigene: 74034 Human
    • Unigene: 28278 Mouse
    • Unigene: 470537 Mouse
    • Alternative names

      • BSCL3 antibody
      • CAV antibody
      • CAV1 antibody
      • CAV1_HUMAN antibody
      • caveolae protein, 22 kD antibody
      • caveolin 1 alpha isoform antibody
      • caveolin 1 beta isoform antibody
      • Caveolin 1 caveolae protein 22kDa antibody
      • Caveolin-1 antibody
      • Caveolin1 antibody
      • cell growth-inhibiting protein 32 antibody
      • CGL3 antibody
      • LCCNS antibody
      • MSTP085 antibody
      • OTTHUMP00000025031 antibody
      • PPH3 antibody
      • VIP 21 antibody
      • VIP21 antibody
      see all

    Images

    • Western blot - Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab192869)
      Western blot - Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab192869)
      All lanes : Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab192869) at 1/1000 dilution

      Lane 1 : Wild-type A-431 (Human epidermoid carcinoma cell line) whole cell lysate
      Lane 2 : CAV1 knockout A-431 (Human epidermoid carcinoma cell line) whole cell lysate
      Lane 3 : A549 (Human lung carcinoma cell line) whole cell lysate
      Lane 4 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 17, 20 kDa
      Observed band size: 21-24 kDa why is the actual band size different from the predicted?



      Lanes 1 - 4: Merged signal (red and green). Green - ab192869 observed at 21-24 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.

      ab192869 was shown to react with Caveolin-1 in wild-type A431 cells in western blot. Loss of signal was observed when CAV1 knockout sample was used. Wild-type and CAV1 knockout A431 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBS-T (0.1% Tween®) before incubation with ab192869 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab192869)
      Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab192869)

      ab192869 staining Caveolin-1 in wild-type HeLa cells (top panel) and CAV1 knockout HeLa cells (ab255371) (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab192869 at 1/500 dilution and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
      Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab192869)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab192869)

      Immunohistochemical analysis of paraffin-embedded Human squamous cell carcinoma of cervix tissue labeling Caveolin-1 using ab192869 at 1/4000 dilution (0.15 μg/ml). A prediluted HRP Polymer for Rabbit IgG was used as the secondary. Counterstain: Hematoxylin. Negative control also shown.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Flow Cytometry (Intracellular) - Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab192869)
      Flow Cytometry (Intracellular) - Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab192869)

      Intracellular Intracellular Flow Cytometry overlay histogram showing wild-type HeLa (green line) and CAV1 knockout HeLa cells (ab255371) stained with ab192869 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab192869) (1x106 in 100µl at 0.04 µg/ml) for 30 min at 22°C. 

      The secondary antibody Goat anti-rabbit IgG H&L (Alexa Fluorr® 488, pre-adsorbed) (ab150081) was used at 1/2000 for 30 min at 22°C. 

      Isotype control antibody was Rabbit IgG (monoclonal) (ab172730) used at the same concentration and conditions as the primary antibody (wild-type HeLa - black line CAV1 knockout HeLa - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity). 

      Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. 

       

       

    • Western blot - Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab192869)
      Western blot - Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab192869)
      All lanes : Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab192869) at 1/10000 dilution

      Lane 1 : A431 cell lysate
      Lane 2 : A549 cell lysate
      Lane 3 : Wild-type HeLa cell lysate
      Lane 4 : CAV1 knockout HeLa cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 17, 20 kDa



      Lanes 1 - 4: Merged signal (red and green). Green - ab192869 observed at 20 kDa. Red - loading control, ab8245 observed at 37 kDa.  

       ab192869 was shown to react with Caveolin-1 in wild-type HeLa. Loss of signal was observed when knockout cell line ab255371 (knockout cell lysate ab263806) was used. Wild-type and Caveolin-1 knockout samples were subjected to SDS-PAGE. ab192869 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 10000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

       

    • Western blot - Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab192869)
      Western blot - Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab192869)
      All lanes : Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab192869) at 1/10000 dilution

      Lane 1 : PC-3 cell lysate
      Lane 2 : A549 cell lysate
      Lane 3 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 17, 20 kDa

    • Immunoprecipitation - Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab192869)
      Immunoprecipitation - Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab192869)

      Immunoprecipitation analysis of A431 cell lysate labeling Caveolin-1 using ab192869 at 1/30 dilution (Lane 1). PBS negative control (Lane 2). Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as the secondary antibody.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab192869)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab192869)

      Immunohistochemical analysis of paraffin-embeddedMouse lung tissue labeling Caveolin-1 using ab192869 at 1/4000 dilution (0.15 μg/ml). A prediluted HRP Polymer for Rabbit IgG was used as the secondary. Counterstain: Hematoxylin. Negative control also shown.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab192869)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab192869)

      Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Caveolin-1 using ab192869 at 1/4000 dilution (0.15 μg/ml). A prediluted HRP Polymer for Rabbit IgG was used as the secondary. Counterstain: Hematoxylin. Negative control also shown.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab192869)
      Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab192869)

      Immunocytochemistry/Immunofluorescence analysis of A-673 cells labelling Caveolin-1 with ab192869 at a dilution of 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. The cells were co-stained with ab195889, Alexa Fluor® 594-conjugated anti-Tubulin [DM1A] at a dilution of 1/200. Nuclei counterstained with DAPI (blue).

    • Flow Cytometry (Intracellular) - Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab192869)
      Flow Cytometry (Intracellular) - Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab192869)

      Intracellular Flow Cytometry analysis of NIH3T3 cells labeling Caveolin-1 using ab192869 at a 1/120 dilution (Red). A Goat anti rabbit IgG (FITC) at 1/150 dilution was used as secondary antibody. Cells were fixed with 2% paraformaldehyde. Cells without incubation with primary antibody and secondary antibody Blue. Rabbit monoclonal IgG was used as isotype control (Black). 

       

       

    • Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab192869)
      Anti-Caveolin-1 antibody [EPR15554] - N-terminal (ab192869)

    Protocols

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (7)

    Publishing research using ab192869? Please let us know so that we can cite the reference in this datasheet.

    ab192869 has been referenced in 7 publications.

    • Streleckiene G  et al. miR-20b and miR-451a Are Involved in Gastric Carcinogenesis through the PI3K/AKT/mTOR Signaling Pathway: Data from Gastric Cancer Patients, Cell Lines and Ins-Gas Mouse Model. Int J Mol Sci 21:N/A (2020). PubMed: 32013265
    • Jäger M  et al. Inducible Knockdown of Endothelial Protein Tyrosine Phosphatase-1B Promotes Neointima Formation in Obese Mice by Enhancing Endothelial Senescence. Antioxid Redox Signal 30:927-944 (2019). PubMed: 29390191
    • Chen W  et al. Clinical implications of hypoxia-inducible factor-1a and caveolin-1 overexpression in isocitrate dehydrogenase-wild type glioblastoma multiforme. Oncol Lett 17:2867-2873 (2019). PubMed: 30854062
    • Bunaciu RP  et al. Retinoic acid and 6-formylindolo(3,2-b)carbazole (FICZ) combination therapy reveals putative targets for enhancing response in non-APL AML. Leuk Lymphoma 60:1697-1708 (2019). PubMed: 30570341
    • Camolotto SA  et al. FoxA1 and FoxA2 drive gastric differentiation and suppress squamous identity in NKX2-1-negative lung cancer. Elife 7:N/A (2018). PubMed: 30475207
    • Costa Verdera H  et al. Cellular uptake of extracellular vesicles is mediated by clathrin-independent endocytosis and macropinocytosis. J Control Release 266:100-108 (2017). WB . PubMed: 28919558
    • Chung KJ  et al. A self-sustained loop of inflammation-driven inhibition of beige adipogenesis in obesity. Nat Immunol 18:654-664 (2017). PubMed: 28414311

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Caveolin-1 antibody [EPR15554] - N-terminal

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (pancreatic cancer PDX)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
    Permeabilization
    No
    Specification
    pancreatic cancer PDX
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
    Fixative
    formalin
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Soledad Camolotto

    Verified customer

    Submitted Mar 30 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Caveolin-1 antibody [EPR15554] - N-terminal

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (lung)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH 6.0
    Permeabilization
    No
    Specification
    lung
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
    Fixative
    formalin
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Soledad Camolotto

    Verified customer

    Submitted Mar 18 2021

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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