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RecombinantRabMAb

Recombinant Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker (ab79397)

Submit a review Q&A (1)References (2)
Western blot - Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker (ab79397)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker (ab79397)
  • Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker (ab79397)
  • Flow Cytometry (Intracellular) - Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker (ab79397)
  • Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker (ab79397)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2220] to Caveolin-2 - Caveolae Marker
  • Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker
    See all Caveolin-2 primary antibodies

  • Description

    Rabbit monoclonal [EPR2220] to Caveolin-2 - Caveolae Marker

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa cell lysate. IHC-P: Human uterus tissue. ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab79397 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/2000.
WB
1/5000 - 1/10000. Predicted molecular weight: 18 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.
Notes
Flow Cyt (Intra)
1/2000.
WB
1/5000 - 1/10000. Predicted molecular weight: 18 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.

Target

  • Function

    May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression.

  • Tissue specificity

    Expressed in endothelial cells, smooth muscle cells, skeletal myoblasts and fibroblasts.

  • Sequence similarities

    Belongs to the caveolin family.

  • Post-translational
    modifications

    Phosphorylated on serine and tyrosine residues. CAV1 promotes phosphorylation on Ser-23 which then targets the complex to the plasma membrane, lipid rafts and caveolae. Phosphorylation on Ser-36 appears to modulate mitosis in endothelial cells (By similarity). Phosphorylation on both Tyr-19 and Tyr-27 is required for insulin-induced 'Ser-727' phosphorylation of STAT3 and its activation. Phosphorylation on Tyr-19 is required for insulin-induced phosphorylation of MAPK1 and DNA binding of STAT3. Tyrosine phosphorylation is induced by both EGF and insulin.

  • Cellular localization

    Nucleus. Cytoplasm. Golgi apparatus membrane. Cell membrane. Membrane > caveola. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. Tyr-19-phosphorylated form is enriched at sites of cell-cell contact and is translocated to the nucleus in complex with MAPK1 in response to insulin (By similarity). Tyr-27-phosphorylated form is located both in the cytoplasm and plasma membrane. CAV1-mediated Ser-23-phosphorylated form locates to the plasma membrane. Ser-36-phosphorylated form resides in intracellular compartments.
  • Information by UniProt

  • Database links

    • Alternative names

      • CAV antibody
      • CAV2 antibody
      • CAV2_HUMAN antibody
      • Caveolae protein 20 kD antibody
      • Caveolin 2 antibody
      • Caveolin 2 isoform a and b antibody
      • Caveolin-2 antibody
      • MGC12294 antibody
      • OTTHUMP00000025032 antibody
      • OTTHUMP00000195982 antibody
      see all

    Images

    • Western blot - Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker (ab79397)
      Western blot - Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker (ab79397)
      Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker (ab79397) at 1/5000 dilution + HeLa cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP conjugated at 1/2000 dilution

      Predicted band size: 18 kDa
      Observed band size: 20 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker (ab79397)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker (ab79397)

      ab79397 at 1/100 dilution staining Caveolin-2 in human uterus by Immunohistochemistry using paraffin-embedded tissue. Note staining of endothelial cells and smooth muscles.

      Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker (ab79397)
      Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker (ab79397)

      ab79397 at 1/100 dilution staining Caveolin-2 in HeLa cells by Immunofluorescence.

    • Flow Cytometry (Intracellular) - Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker (ab79397)
      Flow Cytometry (Intracellular) - Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker (ab79397)

      Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Caveolin-2 (red) with ab79397 at a 1/2000 dilution. Cells were fixed with 80% methanol and permeabilized with 0.1% Tween-20. A goat anti-rabbit IgG (Alexa Fluorr® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies. 

       

       

    • Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker (ab79397)
      Anti-Caveolin-2 antibody [EPR2220] - Caveolae Marker (ab79397)

    References (2)

    Publishing research using ab79397? Please let us know so that we can cite the reference in this datasheet.

    ab79397 has been referenced in 2 publications.

    • Duivenvoorden HM  et al. High-content siRNA 3D co-cultures to identify myoepithelial cell-derived breast cancer suppressor proteins. Sci Data 8:147 (2021). PubMed: 34050191
    • Hashani M  et al. Widespread expression of perilipin 5 in normal human tissues and in diseases is restricted to distinct lipid droplet subpopulations. Cell Tissue Res N/A:N/A (2018). Human . PubMed: 29752569

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    I have purchased several anti-bodies from abcam:
    ab83015
    ab79397
    ab81127
    ab48139
    ab32577
    ab98975.
    I have used the products WB but would now like to use them for IHC. The website says that they have not been tested in this application. Do you know how likely these Abs will be in succeeding if I attempt to do this
    Any help would be much appreaciated.

    Read More
    Answer

    Thank you for contacting us.

    Regarding antibodies ab79397 and ab32577, they both have been tested in IHC with paraffin embedded sections in the species stated on the datasheet. We don’t have preliminary data using them with frozen sections, but they are very likely to react in such conditions.

    Ab48139 has recently been validated to use in IHC-P in Canine, Feline, Mouse, Rat and Human.

    As for ab81127, ab83015 and ab98975, unfortunately, we have not characterised them by IHC, it doesn’t mean they won’t work in this application, but we don’t have data to support it.

    At Abcam we have an antibody evaluation system, called Abreview, by which customers interested in testing an antibody in new species or applications (not yet tested) can benefit from a free primary antibody in their next purchase if they share the results with us, publishing them in our website.

    To participate in this system, customers should first let us know the antibody they want to test in the new species/ applications. We then issue the discount code valid for one free primary antibody. This code must be issued before purchasing the antibody. The customer then purchases the antibody normally, and tests it in the new species / applications.

    Once we receive the results in our Website as an Abreview, the discount code becomes active, and it can be redeemed for any primary antibody ordered within the next 4 months.

    As mentioned, the discount code might be issued before the purchase of the antibody, but, as an exceptional case, I can still send a discount code if you would like to test any of these antibodies (ab81127, ab83015 and ab98975) with IHC and share your results with us.

    To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: http://it.adminsite.com/admin/crm2/www.abcam.com/collaborationdiscount.

    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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