Recombinant Anti-Caveolin-3 antibody [RM1042] (ab289544)

Product name

Anti-Caveolin-3 antibody [RM1042]
See all Caveolin-3 primary antibodies
Description

Rabbit recombinant multiclonal [RM1042] to Caveolin-3
Host species

Rabbit
Tested applications

Suitable for: IHC-P, IHC-Fr, WB, IP, Flow Cyt (Intra), ICC/IFmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

This product was produced with the following immunogens:
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Human, mouse and rat heart lysate. C2C12 differentiated to muscle for 7 days whole cell lysate. RD whole cell lysate. Human skeletal muscle lysate. IHC-P: Human, mouse and rat cardiac muscle tissue. IHC-Fr: Mouse and rat skeletal muscle tissue (fresh). ICC: RD cells. Differentiated C2C12 cells. Flow Cyt (Intra): RD cells. Differentiated C2C12 cells. IP: Human, mouse and rat heart lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Recombinant Multiclonal
Clone number

RM1042
Isotype

IgG
Research areas
- Neuroscience
- Processes

Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Related Products
- VeriBlot for IP Detection Reagent (HRP) (ab131366)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab289544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IHC-P		1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr		1/1000.
WB		1/1000. Predicted molecular weight: 17 kDa.
IP		1/30.
Flow Cyt (Intra)		1/50.
ICC/IF		1/100.

Notes
IHC-P 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr 1/1000.
WB 1/1000. Predicted molecular weight: 17 kDa.
IP 1/30.
Flow Cyt (Intra) 1/50.
ICC/IF 1/100.

Function

May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. May also regulate voltage-gated potassium channels. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress.
Tissue specificity

Expressed predominantly in muscle.
Involvement in disease

Defects in CAV3 are the cause of limb-girdle muscular dystrophy type 1C (LGMD1C) [MIM:607801]. LGMD1C is a myopathy characterized by calf hypertrophy and mild to moderate proximal muscle weakness. LGMD1C inheritance can be autosomal dominant or recessive.
Defects in CAV3 are a cause of hyperCKmia (HYPCK) [MIM:123320]. It is a disease characterized by persistent elevated levels of serum creatine kinase without muscle weakness.
Defects in CAV3 are a cause of rippling muscle disease (RMD) [MIM:606072]. RMD is a rare disorder characterized by mechanically triggered contractions of skeletal muscle. In RMD, mechanical stimulation leads to electrically silent muscle contractions that spread to neighboring fibers that cause visible ripples to move over the muscle.
Defects in CAV3 are a cause of cardiomyopathy familial hypertrophic (CMH) [MIM:192600]; also designated FHC or HCM. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
Defects in CAV3 are the cause of long QT syndrome type 9 (LQT9) [MIM:611818]. Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to excercise or emotional stress. They can present with a sentinel event of sudden cardiac death in infancy.
Defects in CAV3 can be a cause of sudden infant death syndrome (SIDS) [MIM:272120]. SIDS is the sudden death of an infant younger than 1 year that remains unexplained after a thorough case investigation, including performance of a complete autopsy, examination of the death scene, and review of clinical history. Pathophysiologic mechanisms for SIDS may include respiratory dysfunction, cardiac dysrhythmias, cardiorespiratory instability, and inborn errors of metabolism, but definitive pathogenic mechanisms precipitating an infant sudden death remain elusive. Long QT syndromes-associated mutations can be responsible for some SIDS cases.
Sequence similarities

Belongs to the caveolin family.
Cellular localization

Golgi apparatus membrane. Cell membrane. Membrane > caveola. Potential hairpin-like structure in the membrane. Membrane protein of caveolae.
Target information above from: UniProt accession P56539 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 859 Human
- Entrez Gene: 12391 Mouse
- Entrez Gene: 29161 Rat
- Omim: 601253 Human
- SwissProt: P56539 Human
- SwissProt: P51637 Mouse
- SwissProt: P51638 Rat
- Unigene: 98303 Human
Alternative names
- CAV3 antibody
- CAV3_HUMAN antibody
- Caveolin 3 antibody
- Caveolin-3 antibody
- LGMD1C antibody
- LQT9 antibody
- M-caveolin antibody
- MGC126100 antibody
- MGC126101 antibody
- MGC126129 antibody
- OTTHUMP00000115603 antibody
- OTTHUMP00000207105 antibody
- VIP 21 antibody
- VIP21 antibody
see all

Western blot - Anti-Caveolin-3 antibody [RM1042] (ab289544)

All lanes : Anti-Caveolin-3 antibody [RM1042] (ab289544) at 1/1000 dilution

Lane 1 : C2C12 (Mouse myoblasts myoblast) whole cell lysate
Lane 2 : C2C12 differentiated to muscle for 7 days whole cell lysate
Lane 3 : Human heart lysate
Lane 4 : Human kidney lysate
Lane 5 : Mouse heart lysate
Lane 6 : Mouse kidney lysate
Lane 7 : Rat heart lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/5000 dilution

Predicted band size: 17 kDa
Observed band size: 17 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST

Negative control: Kidney (PMID: 8567687).

Higher molecular bands could be sumoylated Caveolin-3. (PMID: 21362625)

Exposure time: 15 seconds.
Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-3 antibody [RM1042] (ab289544)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RD cells labelling Caveolin-3 with ab289544 at 1/100 (4.77 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 (2 µg/mL) dilution (Green). Confocal image showing cytoplasmic staining in RD cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 (2.5 µg/ml) dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 µg/mL) dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [RM1042] (ab289544)

Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labelling Caveolin-3 with ab289544 at 1/5000 (0.095 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on human cardiac muscle is observed. The section was incubated with ab289544 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Immunohistochemistry (Frozen sections) - Anti-Caveolin-3 antibody [RM1042] (ab289544)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat skeletal muscle (fresh) tissue labeling Caveolin-3 with ab289544 at 1/1000 (0.956 µg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 µg/mL) dilution (Green). Positive staining on rat skeletal muscle is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbedat 1/1000 (2 µg/mL) dilution.
Flow Cytometry (Intracellular) - Anti-Caveolin-3 antibody [RM1042] (ab289544)

Flow cytometric analysis of 4% paraformaldehyde fixed 0.1% Tween-20 permeabilized RD (Human muscle spindle cells and large multinucleated cells) cells labelling Caveolin-3 with ab289544 at 1/50 dilution (1 µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-Caveolin-3 antibody [RM1042] (ab289544)

Caveolin-3 was immunoprecipitated from 0.35 mg mouse heart lysate 10 µg with ab289544 at 1/30 dilution (2 µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab289544 at 1/2000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/10000 dilution.

Lane 1: Mouse heart lysate 10 µg

Lane 2: ab289544 IP in mouse heart lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab289544 in mouse heart lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 8 seconds

Dimerized Caveolin-3 was observed at around 34kDa. (PMID: 21362625)
Western blot - Anti-Caveolin-3 antibody [RM1042] (ab289544)

All lanes : Anti-Caveolin-3 antibody [RM1042] (ab289544) at 1/1000 dilution

Lane 1 : RD (Human muscle spindle cells and large multinucleated cells) whole cell lysate
Lane 2 : Human skeletal muscle lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/10000 dilution

Predicted band size: 17 kDa
Observed band size: 17 kDa

Blocking and diluting buffer and concentration: 5% NFDM/TBST

Dimerized Caveolin-3 was observed at around 34kDa. (PMID: 21362625)

Exposure time: 100 seconds
Immunocytochemistry/ Immunofluorescence - Anti-Caveolin-3 antibody [RM1042] (ab289544)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized C2C12 cells labelling Caveolin-3 with ab289544 at 1/100 (4.77 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 (2 µg/mL) dilution (Green). Confocal image showing cytoplasmic and membranous staining in differentiated C2C12 cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 (2.5 µg/ml) dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 µg/mL) dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [RM1042] (ab289544)

Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labelling Caveolin-3 with ab289544 at 1/5000 (0.095 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on mouse cardiac muscle is observed. The section was incubated with ab289544 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [RM1042] (ab289544)

Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labelling Caveolin-3 with ab289544 at 1/5000 (0.095 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on rat cardiac muscle is observed. The section was incubated with ab289544 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [RM1042] (ab289544)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labelling Caveolin-3 with ab289544 at 1/5000 (0.095 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab289544 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Negative control: no staining on human kidney.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [RM1042] (ab289544)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labelling Caveolin-3 with ab289544 at 1/5000 (0.095 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab289544 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Negative control: no staining on mouse kidney.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [RM1042] (ab289544)

Immunohistochemical analysis of paraffin-embedded rat kidney tissue labelling Caveolin-3 with ab289544 at 1/5000 (0.095 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab289544 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Negative control: no staining on rat kidney.
Immunohistochemistry (Frozen sections) - Anti-Caveolin-3 antibody [RM1042] (ab289544)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse skeletal muscle (fresh) tissue labeling Caveolin-3 with ab289544 at 1/1000 (0.956 µg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 µg/mL) dilution (Green). Positive staining on mouse skeletal muscle is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 µg/mL) dilution.
Flow Cytometry (Intracellular) - Anti-Caveolin-3 antibody [RM1042] (ab289544)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized C2C12 (Mouse myoblasts myoblast) differentiated to muscle for 7 days (Right) / Undifferentiated C2C12 cells (Left). Cells labelling Caveolin-3 with ab289544 at 1/500 dilution (0.1 µg)/ Left and Right. Goat Anti-Rabbit IgG (Alexa Fluor^® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-Caveolin-3 antibody [RM1042] (ab289544)

Caveolin-3 was immunoprecipitated from 0.35 mg human heart lysate 10 µg with ab289544 at 1/30 dilution (2 µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab289544 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: Human heart lysate 10 µg

Lane 2: ab289544 IP in human heart lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab289544 in human heart lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 seconds
Immunoprecipitation - Anti-Caveolin-3 antibody [RM1042] (ab289544)

Caveolin-3 was immunoprecipitated from 0.35 mg rat heart lysate 10 µg with ab289544 at 1/30 dilution (2 µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab289544 at 1/2000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/10000 dilution.

Lane 1: Rat heart lysate 10 µg

Lane 2: ab289544 IP in rat heart lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab289544 in rat heart lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 8 seconds

Dimerized Caveolin-3 was observed at around 34kDa. (PMID: 21362625)
Anti-Caveolin-3 antibody [RM1042] (ab289544)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

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Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Compatible Secondaries

Isotype control

Related Products

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Involvement in disease

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

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