Recombinant Anti-Caveolin-3 antibody [RM1042] (ab289544)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit recombinant multiclonal [RM1042] to Caveolin-3
- Suitable for: IHC-P, IHC-Fr, WB, IP, Flow Cyt (Intra), ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Caveolin-3 antibody [RM1042]
See all Caveolin-3 primary antibodies -
Description
Rabbit recombinant multiclonal [RM1042] to Caveolin-3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IHC-Fr, WB, IP, Flow Cyt (Intra), ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
This product was produced with the following immunogens:
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers. -
Positive control
- WB: Human, mouse and rat heart lysate. C2C12 differentiated to muscle for 7 days whole cell lysate. RD whole cell lysate. Human skeletal muscle lysate. IHC-P: Human, mouse and rat cardiac muscle tissue. IHC-Fr: Mouse and rat skeletal muscle tissue (fresh). ICC: RD cells. Differentiated C2C12 cells. Flow Cyt (Intra): RD cells. Differentiated C2C12 cells. IP: Human, mouse and rat heart lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Recombinant Multiclonal -
Clone number
RM1042 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab289544 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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IHC-Fr |
1/1000.
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WB |
1/1000. Predicted molecular weight: 17 kDa.
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IP |
1/30.
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|
Flow Cyt (Intra) |
1/50.
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|
ICC/IF |
1/100.
|
Notes |
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IHC-P
1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
IHC-Fr
1/1000. |
WB
1/1000. Predicted molecular weight: 17 kDa. |
IP
1/30. |
Flow Cyt (Intra)
1/50. |
ICC/IF
1/100. |
Target
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Function
May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. May also regulate voltage-gated potassium channels. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress. -
Tissue specificity
Expressed predominantly in muscle. -
Involvement in disease
Defects in CAV3 are the cause of limb-girdle muscular dystrophy type 1C (LGMD1C) [MIM:607801]. LGMD1C is a myopathy characterized by calf hypertrophy and mild to moderate proximal muscle weakness. LGMD1C inheritance can be autosomal dominant or recessive.
Defects in CAV3 are a cause of hyperCKmia (HYPCK) [MIM:123320]. It is a disease characterized by persistent elevated levels of serum creatine kinase without muscle weakness.
Defects in CAV3 are a cause of rippling muscle disease (RMD) [MIM:606072]. RMD is a rare disorder characterized by mechanically triggered contractions of skeletal muscle. In RMD, mechanical stimulation leads to electrically silent muscle contractions that spread to neighboring fibers that cause visible ripples to move over the muscle.
Defects in CAV3 are a cause of cardiomyopathy familial hypertrophic (CMH) [MIM:192600]; also designated FHC or HCM. Familial hypertrophic cardiomyopathy is a hereditary heart disorder characterized by ventricular hypertrophy, which is usually asymmetric and often involves the interventricular septum. The symptoms include dyspnea, syncope, collapse, palpitations, and chest pain. They can be readily provoked by exercise. The disorder has inter- and intrafamilial variability ranging from benign to malignant forms with high risk of cardiac failure and sudden cardiac death.
Defects in CAV3 are the cause of long QT syndrome type 9 (LQT9) [MIM:611818]. Long QT syndromes are heart disorders characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to excercise or emotional stress. They can present with a sentinel event of sudden cardiac death in infancy.
Defects in CAV3 can be a cause of sudden infant death syndrome (SIDS) [MIM:272120]. SIDS is the sudden death of an infant younger than 1 year that remains unexplained after a thorough case investigation, including performance of a complete autopsy, examination of the death scene, and review of clinical history. Pathophysiologic mechanisms for SIDS may include respiratory dysfunction, cardiac dysrhythmias, cardiorespiratory instability, and inborn errors of metabolism, but definitive pathogenic mechanisms precipitating an infant sudden death remain elusive. Long QT syndromes-associated mutations can be responsible for some SIDS cases. -
Sequence similarities
Belongs to the caveolin family. -
Cellular localization
Golgi apparatus membrane. Cell membrane. Membrane > caveola. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. - Information by UniProt
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Database links
- Entrez Gene: 859 Human
- Entrez Gene: 12391 Mouse
- Entrez Gene: 29161 Rat
- Omim: 601253 Human
- SwissProt: P56539 Human
- SwissProt: P51637 Mouse
- SwissProt: P51638 Rat
- Unigene: 98303 Human
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Alternative names
- CAV3 antibody
- CAV3_HUMAN antibody
- Caveolin 3 antibody
see all
Images
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All lanes : Anti-Caveolin-3 antibody [RM1042] (ab289544) at 1/1000 dilution
Lane 1 : C2C12 (Mouse myoblasts myoblast) whole cell lysate
Lane 2 : C2C12 differentiated to muscle for 7 days whole cell lysate
Lane 3 : Human heart lysate
Lane 4 : Human kidney lysate
Lane 5 : Mouse heart lysate
Lane 6 : Mouse kidney lysate
Lane 7 : Rat heart lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/5000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Negative control: Kidney (PMID: 8567687).
Higher molecular bands could be sumoylated Caveolin-3. (PMID: 21362625)
Exposure time: 15 seconds.
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RD cells labelling Caveolin-3 with ab289544 at 1/100 (4.77 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 µg/mL) dilution (Green). Confocal image showing cytoplasmic staining in RD cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 µg/ml) dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/mL) dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [RM1042] (ab289544)
Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labelling Caveolin-3 with ab289544 at 1/5000 (0.095 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on human cardiac muscle is observed. The section was incubated with ab289544 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat skeletal muscle (fresh) tissue labeling Caveolin-3 with ab289544 at 1/1000 (0.956 µg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/mL) dilution (Green). Positive staining on rat skeletal muscle is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 (2 µg/mL) dilution.
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Flow cytometric analysis of 4% paraformaldehyde fixed 0.1% Tween-20 permeabilized RD (Human muscle spindle cells and large multinucleated cells) cells labelling Caveolin-3 with ab289544 at 1/50 dilution (1 µg) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
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Caveolin-3 was immunoprecipitated from 0.35 mg mouse heart lysate 10 µg with ab289544 at 1/30 dilution (2 µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab289544 at 1/2000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/10000 dilution.
Lane 1: Mouse heart lysate 10 µg
Lane 2: ab289544 IP in mouse heart lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab289544 in mouse heart lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 8 seconds
Dimerized Caveolin-3 was observed at around 34kDa. (PMID: 21362625)
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All lanes : Anti-Caveolin-3 antibody [RM1042] (ab289544) at 1/1000 dilution
Lane 1 : RD (Human muscle spindle cells and large multinucleated cells) whole cell lysate
Lane 2 : Human skeletal muscle lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/10000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST
Dimerized Caveolin-3 was observed at around 34kDa. (PMID: 21362625)
Exposure time: 100 seconds
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized C2C12 cells labelling Caveolin-3 with ab289544 at 1/100 (4.77 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 µg/mL) dilution (Green). Confocal image showing cytoplasmic and membranous staining in differentiated C2C12 cells. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 µg/ml) dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/mL) dilution.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [RM1042] (ab289544)
Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labelling Caveolin-3 with ab289544 at 1/5000 (0.095 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on mouse cardiac muscle is observed. The section was incubated with ab289544 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [RM1042] (ab289544)
Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labelling Caveolin-3 with ab289544 at 1/5000 (0.095 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Membranous staining on rat cardiac muscle is observed. The section was incubated with ab289544 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [RM1042] (ab289544)
Immunohistochemical analysis of paraffin-embedded human kidney tissue labelling Caveolin-3 with ab289544 at 1/5000 (0.095 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab289544 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Negative control: no staining on human kidney.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [RM1042] (ab289544)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labelling Caveolin-3 with ab289544 at 1/5000 (0.095 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab289544 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Negative control: no staining on mouse kidney.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caveolin-3 antibody [RM1042] (ab289544)
Immunohistochemical analysis of paraffin-embedded rat kidney tissue labelling Caveolin-3 with ab289544 at 1/5000 (0.095 µg/ml) followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab289544 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.
Negative control: no staining on rat kidney.
-
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse skeletal muscle (fresh) tissue labeling Caveolin-3 with ab289544 at 1/1000 (0.956 µg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/mL) dilution (Green). Positive staining on mouse skeletal muscle is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/mL) dilution.
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Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized C2C12 (Mouse myoblasts myoblast) differentiated to muscle for 7 days (Right) / Undifferentiated C2C12 cells (Left). Cells labelling Caveolin-3 with ab289544 at 1/500 dilution (0.1 µg)/ Left and Right. Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.
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Caveolin-3 was immunoprecipitated from 0.35 mg human heart lysate 10 µg with ab289544 at 1/30 dilution (2 µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab289544 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Human heart lysate 10 µg
Lane 2: ab289544 IP in human heart lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab289544 in human heart lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds
-
Caveolin-3 was immunoprecipitated from 0.35 mg rat heart lysate 10 µg with ab289544 at 1/30 dilution (2 µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab289544 at 1/2000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/10000 dilution.
Lane 1: Rat heart lysate 10 µg
Lane 2: ab289544 IP in rat heart lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab289544 in rat heart lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 8 seconds
Dimerized Caveolin-3 was observed at around 34kDa. (PMID: 21362625)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab289544 has not yet been referenced specifically in any publications.