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RabMAb

Anti-CBL (phospho Y700) antibody [EP2225Y] (ab76002)

Submit a review Submit a question References (1)
Western blot - Anti-CBL (phospho Y700) antibody [EP2225Y] (ab76002)
  • Flow Cytometry (Intracellular) - Anti-CBL (phospho Y700) antibody [EP2225Y] (ab76002)

Key features and details

  • Rabbit monoclonal [EP2225Y] to CBL (phospho Y700)
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Human
  • Isotype: IgG

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-CBL (phospho Y700) antibody [EP2225Y]
    See all CBL primary antibodies

  • Description

    Rabbit monoclonal [EP2225Y] to CBL (phospho Y700)

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), WBmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Jurkat cell lysate, treated with pervanadate Flow Cyt: Permeabilized Jurkat cells, treated with pervanadate.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab76002 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/5000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 99 kDa).
Notes
Flow Cyt (Intra)
1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/5000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 99 kDa).
Application notes
Is unsuitable for IHC-P.

Target

  • Function

    Participates in signal transduction in hematopoietic cells. Adapter protein that functions as a negative regulator of many signaling pathways that start from receptors at the cell surface. Acts as an E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Recognizes activated receptor tyrosine kinases, including PDGFA, EGF and CSF1, and terminates signaling.

  • Pathway

    Protein modification; protein ubiquitination.

  • Involvement in disease

    Defects in CBL are the cause of Noonan syndrome-like disorder (NSL) [MIM:613563]. NSL is a syndrome characterized by a phenotype reminiscent of Noonan syndrome. Clinical features are highly variable, including facial dysmorphism, short neck, developmental delay, hyperextensible joints and thorax abnormalities with widely spaced nipples. The facial features consist of triangular face with hypertelorism, large low-set ears, ptosis, and flat nasal bridge. Some patients manifest cardiac defects.

  • Sequence similarities

    Contains 1 Cbl-PTB (Cbl-type phosphotyrosine-binding) domain.
    Contains 1 RING-type zinc finger.
    Contains 1 UBA domain.

  • Domain

    The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.
    The N-terminus is composed of the phosphotyrosine binding (PTB) domain, a short linker region and the RING-type zinc finger. The PTB domain, which is also called TKB (tyrosine kinase binding) domain, is composed of three different subdomains: a four-helix bundle (4H), a calcium-binding EF hand and a divergent SH2 domain.

  • Post-translational
    modifications

    Phosphorylated on tyrosine residues by EGFR, SYK, FYN and ZAP70 (By similarity). Phosphorylated on tyrosine residues by INSR.

  • Cellular localization

    Cytoplasm.
  • Information by UniProt

  • Database links

    • Alternative names

      • 4732447J05Rik antibody
      • C CBL antibody
      • Cas Br M (murine) ecotropic retroviral transforming sequence antibody
      • Casitas B lineage lymphoma proto oncogene antibody
      • Casitas B-lineage lymphoma proto-oncogene antibody
      • CBL 2 antibody
      • cbl antibody
      • CBL_HUMAN antibody
      • CBL2 antibody
      • E3 ubiquitin protein ligase CBL antibody
      • E3 ubiquitin-protein ligase CBL antibody
      • Oncogene CBL2 antibody
      • Proto oncogene c CBL antibody
      • Proto-oncogene c-CBL antibody
      • RGD1561386 antibody
      • RING finger protein 55 antibody
      • RNF55 antibody
      • Signal transduction protein CBL antibody
      see all

    Images

    • Western blot - Anti-CBL (phospho Y700) antibody [EP2225Y] (ab76002)
      Western blot - Anti-CBL (phospho Y700) antibody [EP2225Y] (ab76002)
      All lanes : Anti-CBL (phospho Y700) antibody [EP2225Y] (ab76002) at 1/10000 dilution

      Lane 1 : Jurkat cell lysate, untreated
      Lane 2 : Jurkat cell lysate, treated with pervanadate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 99 kDa
      Observed band size: 120 kDa why is the actual band size different from the predicted?

    • Flow Cytometry (Intracellular) - Anti-CBL (phospho Y700) antibody [EP2225Y] (ab76002)
      Flow Cytometry (Intracellular) - Anti-CBL (phospho Y700) antibody [EP2225Y] (ab76002)

      Intracellular flow cytometric analysis of ab76002 at 1/50 dilution staining permeabilized Jurkat cells, untreated (green) or pervanadate-treated (red) and pervanadate-treated Jurkat cells preincubated with immunising phospho-peptide (blue) or non-phospho peptide (orange).

       

    References (1)

    Publishing research using ab76002? Please let us know so that we can cite the reference in this datasheet.

    ab76002 has been referenced in 1 publication.

    • Greene W  et al. The ubiquitin/proteasome system mediates entry and endosomal trafficking of Kaposi's sarcoma-associated herpesvirus in endothelial cells. PLoS Pathog 8:e1002703 (2012). ICC/IF . PubMed: 22615563

    Customer reviews and Q&As

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