Recombinant Anti-CBR1 antibody [EPR9660] (ab156590)
![Western blot - Anti-CBR1 antibody [EPR9660] (ab156590)](https://www.abcam.com/ps/products/156/ab156590/Images/ab156590-397645-anti-cbr1-antibody-epr9660-western-blot-human-knockout-lysates.jpg "Western blot - Anti-CBR1 antibody [EPR9660] (ab156590)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9660] to CBR1
- Suitable for: WB, IHC-P, ICC/IF
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CBR1 antibody [EPR9660]
See all CBR1 primary antibodies -
Description
Rabbit monoclonal [EPR9660] to CBR1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, 293T, SH-SY5Y and Human fetal kidney lysates; Human kidney tissue; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9660 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab156590 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 30 kDa.
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| ICC/IF |
1/50 - 1/100.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 30 kDa. |
|
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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ICC/IF
1/50 - 1/100. |
Target
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Function
NADPH-dependent reductase with broad substrate specificity. Catalyzes the reduction of a wide variety of carbonyl compounds including quinones, prostaglandins, menadione, plus various xenobiotics. Catalyzes the reduction of the antitumor anthracyclines doxorubicin and daunorubicin to the cardiotoxic compounds doxorubicinol and daunorubicinol. Can convert prostaglandin E2 to prostaglandin F2-alpha. Can bind glutathione, which explains its higher affinity for glutathione-conjugated substrates. Catalyzes the reduction of S-nitrosoglutathione. -
Sequence similarities
Belongs to the short-chain dehydrogenases/reductases (SDR) family. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 873 Human
- Omim: 114830 Human
- SwissProt: P16152 Human
- Unigene: 606200 Human
- Unigene: 88778 Human
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Alternative names
- 15 hydroxyprostaglandin dehydrogenase [NADP+] antibody
- 15-hydroxyprostaglandin dehydrogenase [NADP+] antibody
- Carbonyl reductase [NADPH] 1 antibody
see all
Images
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Western blot - Anti-CBR1 antibody [EPR9660] (ab156590)All lanes : Anti-CBR1 antibody [EPR9660] (ab156590) at 1/1000 dilution
Lane 1 : Wild-type A549 cell lysate
Lane 2 : CBR1 knockout A549 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDaLanes 1-3: Merged signal (red and green). Green - ab156590 observed at 30 kDa. Red - loading control ab8245 observed at 36 kDa.
ab156590 Anti-CBR1 antibody [EPR9660] was shown to specifically react with CBR1 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab266954 (knockout cell lysate ab257874) was used. Wild-type and CBR1 knockout samples were subjected to SDS-PAGE. ab156590 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBR1 antibody [EPR9660] (ab156590)](https://www.abcam.com/ps/products/156/ab156590/Images/ab156590-168880-anti-cbr1-antibody-epr9660-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBR1 antibody [EPR9660] (ab156590)ab156590 showing +ve staining in Human thyroid gland carcinoma.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-CBR1 antibody [EPR9660] (ab156590)](https://www.abcam.com/ps/products/156/ab156590/Images/ab156590-163735-anti-cbr1-antibody-epr9660-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CBR1 antibody [EPR9660] (ab156590)Immunofluorescent analysis of HeLa cells labeling CBR1 with ab156590 at 1/50 dilution. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBR1 antibody [EPR9660] (ab156590)](https://www.abcam.com/ps/products/156/ab156590/Images/ab156590-168879-anti-cbr1-antibody-epr9660-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBR1 antibody [EPR9660] (ab156590)ab156590 showing +ve staining in Human skeletal muscle.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBR1 antibody [EPR9660] (ab156590)](https://www.abcam.com/ps/products/156/ab156590/Images/ab156590-168878-anti-cbr1-antibody-epr9660-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBR1 antibody [EPR9660] (ab156590)ab156590 showing +ve staining in Human gastric carcinoma.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBR1 antibody [EPR9660] (ab156590)](https://www.abcam.com/ps/products/156/ab156590/Images/ab156590-163736-anti-cbr1-antibody-epr9660-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CBR1 antibody [EPR9660] (ab156590)Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling CBR1 with ab156590 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Western blot - Anti-CBR1 antibody [EPR9660] (ab156590)](https://www.abcam.com/ps/products/156/ab156590/Images/ab156590-163737-anti-cbr1-antibody-epr9660-western-blot.jpg)
Western blot - Anti-CBR1 antibody [EPR9660] (ab156590)All lanes : Anti-CBR1 antibody [EPR9660] (ab156590) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : SH-SY5Y cell lysate
Lane 4 : Human fetal kidney lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 30 kDa -
![Anti-CBR1 antibody [EPR9660] (ab156590)](https://www.abcam.com/ps/products/156/ab156590/Images/ab156590-8-benefits-of-recombinant-antibodies.png)
Anti-CBR1 antibody [EPR9660] (ab156590)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab156590 has been referenced in 2 publications.
- Kwon JH et al. Upregulation of Carbonyl Reductase 1 by Nrf2 as a Potential Therapeutic Intervention for Ischemia/ Reperfusion Injury during Liver Transplantation. Mol Cells 42:672-685 (2019). PubMed: 31486328
- Yamanouchi R et al. Low carbonyl reductase 1 expression is associated with poor prognosis in patients with oral squamous cell carcinoma. Mol Clin Oncol 8:400-406 (2018). PubMed: 29456845